Extracellular vesicles (EV) are thought to mediate the systemic adaptations to physical exercise including running. In this systematic review, we aimed to analyze the kinetics of EV release in peripheral blood in runners. We searched Pubmed, Scopus and Web of Science using the search strategy [‘Extracellular vesicles’ OR ‘exosomes’] AND [‘running’ OR ‘treadmill’ OR ‘marathon’] according to PRISMA guidelines. We selected the studies that assessed the EV numbers or size in response to running in humans. Conference abstracts, reviews, studies published in languages other than English, studies assessing any EV components without EV isolation, and EV studies in experimental animal models were excluded. There were nine studies (#1-9), with a total of 152 runners, including 30 marathon runners. Running activities included treadmill test (#1-7) and high intensity outdoor running (#8, #9). The EV release was observed during running (#1, n=16), immediately after running (#2, n=10; #3, n=12; #5, n=8; #7, n=19; #9, n=26), remained elevated within 90 min (#3, n=12) or decreased overnight (#6, n=10; #8, n=27). There was no effect of running on the EV numbers in #4 (n=9). Size-exclusion chromatography was used for EV isolation (#2; #4), nanoparticle tracking analysis – for EV quantification (#3-7) and Western blotting - for EV cargo analysis (#2-4; #6-7). The optimal timing for EV analysis was within 90 min after running, although a protocol standardization for EV analysis is needed. This work was carried out in support of our ongoing research into the EV release in runners with or without urticaria.

ESDR072 - The release of extracellular vesicles in runners: a systematic review

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