Data for "Condensin dysfunction and peri-centromere expansion create a reproductive isolating barrier in mice"

Microscopy and Western blot images analyzed in the Figures and Extended Data Figures in El Yakoubi and Akera, "Condensin dysfunction and peri-centromere expansion create a reproductive isolating barrier in mice"

Abstract:

Reproductive isolation occurs when the genomes of two populations accumulate genetic incompatibilities that prevent inter-breeding. Cell biological understanding of such hybrid incompatibility is limited, especially for hybrid female sterility. Here we find that species divergence in condensin regulation and centromere organization between two mouse species, Mus musculus domesticus and Mus spretus, drives chromosome de-condensation and mis-segregation in their F1 hybrid oocytes, reducing female fertility. The chromosome condensation defects in hybrid oocytes were especially prominent at Mus musculus domesticus centromeres due to their highly abundant peri-centromeric major satellite DNA, leading to species-specific chromosome mis-segregation. This study provides the first mechanistic insights into hybrid incompatibility in female meiosis and demonstrates that the combination of condensin mis-regulation and peri-centromere expansion can establish a reproductive isolating barrier in mammals.