2019 Volume 18 Issue 3 Pages 227-233
Aloe tomentosa Deflers takes approximately 10 years to progress from germination to seed production. In the micropropagation of A. tomentosa, hyperhydricity and somaclonal variation have not yet been successfully controlled. To solve these problems, we used aseptic germination and the axillary branching method in MS medium without plant growth regulators. Using the aseptic germination technique, 47% of the seedlings were normal. Using the axillary branching method, the average number of axillary buds that elongated from explants was 6.4, and addition of 6-benzylaminopurine (BAP) to the medium induced hyperhydrity. These shoots were easily rooted and grown in an unheated greenhouse after acclimation. These results suggest that aseptic seeding germination and the axillary branching method without plant growth regulators were effective propagation methods.