Radiochemical Technology for Production of Preparations of Technetium-99m on Extraction Centrifugal Semi-Countercurrent Generator

The results of the development and long-term operation of the technetium-99m extraction generator and use of Radiopharmaceuticals (RP) prepared on its base are presented. The main advantage of such generator is the possibility of separation of the molybdenum and technetium isotopes obtained with the use of any starting materials (both enriched and natural stable isotopes) as well as any irradiators (reactor and accelerators) to obtain the product sodium [99mTc] pertechnetate solution with the necessary radioactive concentration and radiochemical purity ≥ 95%.

The classical semi-countercurrent process and its calculations are well known [1,2]. The semi-counter current extraction process of separation of elements in a dynamic mode was first described in 1951 [3].
The main feature of semi-countercurrent separation method is the fact that, if the initial mixture of the components is in the aqueous phase, than the less extractable component can be obtained with any purity level, but the yield continuously decreases. At the same time, the more extracted component has a maximum of the concentration rate equal to the value of the separation coefficient at the beginning of the extraction process.
In semi-counter current extractors of the mixer-settler types with gravitational phase separation were used [1][2][3]. The main disadvantages of such devices are high retention volume of the mobile phase, low efficiency and productivity, as well as the possibility of entrainment of the emulsion.
Semi-countercurrent processes are used in the separation of similar components in Craig apparatuses, but they are of little use for radiochemical purposes for many reasons: low stratification rate, complex and cumbersome construction, long time needed to achieve equilibrium when the process of mixing is simple wiggle.
One of the first papers describing the use of centrifugal semi-countercurrent extractors for the separation of multi-component mixtures requiring a large number of stages was published in 1974 by GV Korpusov et al., [4]. The centrifugal extractor considered is not intended to be used for obtaining individual radionuclide's, since in this case the requirements for the purity of the product are much more stringent at a high recovery rate [4].
Considering the need to implement the process of isolation of short-lived isotopes in a short period of time, semi-counter flow extractors with forced phase separation in a centrifugal field were developed. The use of such apparatuses makes it possible to shorten the time of the process and increase the purity of the products, since in this case intensive mixing is acceptable (equilibrium in the extraction system is achieved in 2-3 seconds) and phase separation in the centrifugal field excludes the entrainment of the emulsion.
The process of continuous organic solvent feeding into the initial aqueous solution, containing the components to be recovered, is realized in the extractor.
Passing through the mixing chamber and the separator, the solvent sequentially extracts the components of the mixture in accordance with the decrease in their distribution coefficients (D 2 > D 1 ) (Figures 1 and 2).
The organic phase can be washed in the same manner, but in this case the less extractable component of the mixture is washed out first (1/D 1 ; 1/D 2 ). Both processes can be described by the following equations [5]:

Extraction
(1) Washing (2) Where: σ w , σ o -relative concentrations of the extracted or washed out components in the aqueous (in the case of extraction) and organic (in the case of washing) phases; C°, C -initial and final concentrations of the extracted component ĉ°, ĉ -initial and final concentrations of the washed out component D -Distribution coefficient W -Feed rate of extractant W°-Volume of initial extract V°-Volume of initial aqueous phase V -feed rate of washing solution r w , r o -the ratio of phases in the emulsion during extraction and washing processes, respectively ; Depending on the task, there are two types of these devices: with stationary aqueous or organic phase. The calculation methods are the same, and there is only one thing that changes: the inverse values of the distribution coefficients (1/D) are used for calculations in the case of a stationary organic phase.
The semi-countercurrent extraction process is carried out as follows: a stationary phase (aqueous or organic) containing a mixture of elements to be separated is introduced into the apparatus in a certain volume. After that a stream of extractant or aqueous solution, respectively is passed through it. The effective operation of the extraction generators of radioactive isotopes is equally depends on the selected extraction system, the properly organized process of isolation and the design features of the extractor.
A number of specific requirements are presented to extraction generators used for the production of radionuclides: • Complete elimination of phase entrainment • Absence of stagnation zones

• Possibility of phase inversion
The design of the developed extraction centrifugal apparatus must also satisfy certain functional requirements: • Possibility of combining the basic typical operations in one device (extraction, washing, re-extraction and fraction collection) • Functional adjustment of isolated chambers with the possibility of independent filling, emptying and rinsing • Creation of conditions for additional clarification of outflow solutions

Modern Approaches to Overcoming the "Molybdenum Crisis"
Technetium-99m is one of the most widely used radionuclides in diagnostic nuclear medicine during the entire period of formation and development of nuclear medicine. Technetium-99m decays emitting only gamma rays with photon energy of 0.14 MeV. This low energy, as well as the absence of β -radiation and a short half-life (6.02 h) minimizes radiation hazard of technetium-99m for the patient [6].
The parent isotope for the production of technetium-99m is molybdenum-99, which can be obtained by irradiation of natural or enriched molybdenum-98 with neutrons and also can be isolated from the mixture of fission products of uranium-235. Obtaining 99 Mo from irradiated uranium is an independent, expensive, complex problem requiring deep purification of the final product from radionuclide impurities, such as 131 I, 103 Ru, 95 Zr, etc. Practically all survey papers on 99m Tc and 99 Mo contain information on the advantages and disadvantages of both nuclear reactions. In both cases, the target must be   ( ) irradiated in a nuclear reactor with a neutron flux of more than 5×10 13 n/ (cm 2 .s) [7].
Since 2007, in the context of the world-wide "Molybdenum crisis" (which was caused by the almost simultaneous shutdown of reactors in Canada and Holland, that providing more than 60% of the world production of 99 Mo), alternative ways of molybdenum-99 production using accelerators took another development [8][9][10]. 99 Mo can be produced by the reaction 100 Mo (p, pn) 99 Mo, as well as 99m Tc directly, with high yields, using the charged particle accelerators (for example see [9]). Several studies have been published on the determination of the yields of various radionuclides during irradiation of molybdenum targets by protons [11], deuterons [12] and photonuclear reactions were also studied [13,14]. In the review the problems, advantages and disadvantages of all currently available sources and possibilities for obtaining 99m Tc are discussed in detail, since the exceptional value and necessity of this radionuclide in modern nuclear medicine does not lose its significance [15]. Now several research projects are being implemented in this field and leading manufacturers of medium-energy cyclotrons (GE Healthcare, IBA and Advanced Cyclotron Systems Inc.) actively offer to their customers to consider the possibility of technetium-99m production in operating PET-centers.
The most complete studies were carried out by a group of Canadian specialists who has now completed preclinical studies of specific radiopharmaceuticals with cyclotron produced 99m Tc. The results of first clinical trials were published as well. The effect of radionuclide impurities, formed during nuclear reactions on accelerators, on radiation dose received by patients and the quality of SPECT imaging were studied [16][17][18].
When 99m Tc is obtained from 99 Mo produced on the reactor or accelerator, or when 99m Tc is directly obtained as a result of the nuclear reaction, there is a problem of the subsequent separation of technetium and molybdenum isotopes. The use of a semi-countercurrent centrifugal extractor for solving this problem is discussed below.

Selection of the Extraction System for the Separation of 99 Mo and 99m Tc
In addition to sorption methods, which are extremely useful in the case of preparing a generator for clinical use, the extraction can also be used to separate molybdenum and technetium. The regularities of the extraction separation of 99 Mo and 99m Tc in various systems have been studied in detail as early as the 1960s [19]. For the extraction separation of this pair of elements, it is possible to use various extractants, such as MEK, methyl isobutyl ketone, as well as organ phosphorus compounds [20,21]. In this case, the distribution coefficients of these elements differ by 2-3 orders of magnitude, which make it possible to achieve high values of Separation Coefficients (K s ). For example, when extracting 99m Tc from 5 M NaOH solution in MEK (100%), the value of K s = 2×10 5 and from 5 M K 2 CO 3 К s = 4×10 5 . Therefore, a semi-countercurrent process can be performed on a limited number of steps (from 1 to 3). It is rational to use a mixture of alkali and carbonate, which plays the role of a salting out agent. One of the first technological schemes on the basis of this system for obtaining 99m Tc of pharmaceutical quality in a countercurrent glass column was published by R Boyd in 1982 [22]. Later this scheme was applied in different countries. During the extraction separation of 99m Tc and 99 Mo using 100% MEK, the extract is evaporated to dryness, and the dry residue containing 99m Tc is dissolved in 0.9% NaCl solution. According to the requirements of US Pharmacopeia a relatively high MEK content (≤ 0.1%) in the product (sodium [ 99m Tc] pertechnetate) is allowed [23].
It should be noted that in the case of use of enriched molybdenum targets for irradiation with necessity of following their regeneration for repeated irradiation, only potassium should be used as the alkali metal. This is necessary to exclude the possibility of sodium traces presence in the target and correspondingly, radionuclide impurities presence in the final product.

Two-Stage Extraction Centrifugal Semi-Countercurrent Generator for 99m Tc Production
To implement the process of 99m Tc production for radiopharmaceuticals, a centrifugal two-stage extractor integrating the extraction and washing stages was created ( Figure 1). The generator works as follows; a certain volume of the initial solution in the form of sodium (or potassium) molybdate is introduced into the mixing chamber (9) of the lower stage through the central tube (10). The second stage is filled with a washing solution (5 M K 2 CO 3 ) through the tube (11).
After filling the apparatus, an electric motor is started using a starting device. Electric motor rotates the generator housing (2). During these operations the tube (11), as well as the central tube (10), does not come into contact with the solutions in the chambers (6) and (9), because they are thrown to the periphery by centrifugal forces. Then, the dosing device is turned on and the extractant (MEK) is fed to the mixing chamber (9) via the central tube (10) with a predetermined flow rate. In the mixing chambers (9) and (6) uniform mixing of the phases occurs with the stirrers (8) and (5) respectively. The resulting emulsion exits from the mixing chamber (9) through the annular gap (15) and enters the stratifying chamber (7) where it is stratified to form light and heavy phases under the action of centrifugal forces.
As the light phase exits the mixing chamber (9) to enter into the stratifying chamber (7), it reaches the level of the water seal (14) and then it rises along the inner surface of the cylinder (4) and enters the second mixing chamber (6). The height and inner diameter of the cylinder (4) are preliminarily determined by calculation. Depleted on the light phase emulsion in the stratifying chamber (7) through the annular gap (15) returns again to the mixing chamber (9), creating recirculation.
In the second (washing) stage, the hydrodynamic processes are repeated and the light phase ( 99m Tc extract) comes from the stratifying chamber (3) into the annular groove (12) from which it is withdrawn from the extractor with the tube (1).
The main structural material of the extractor was stainless steel. Stirring devices which contacting with solutions was made of inert metals: niobium, tantalum and zirconium.
The extractor, with stages located on one rotation axis in the vertical position, is attached to the electric motor shaft. The operating speed is 2000-2500 rpm.
It should be noted that the extractor has high reliability in operation due to the fact that there are no rubbing points or contact points between the rotating drum and fixed parts (tubes, mixers). The operating time of the apparatus is determined by the engine life. The radiation dose from 99 Mo and 99m Tc virtually do not affect the life of the engine. Figure 2 shows the dependence of the 99m Tc extraction degree on the fed extractant volume. As can be seen from the data given, in the chosen mode, 50-60 ml of extractant is sufficient to extract 95% of Tc. At an organic phase feed rate of 600-1000 ml/h, the time of the extraction course will be 5-10 minutes, respectively.

Extraction Apparatus for the Separation of Technetium-99m from the Irradiated Molybdenum Matrix
To obtain the final sodium [ 99m Tc] pertechnetate, it is required to use an installation that includes, apart from the extractor, additional processing equipment. The diagram of such an installation is shown in figure 3.
The connection of all vessels and pumps is carried out by chemically stable and flexible polymer pipes with an internal diameter of 2-5 mm.
The initial solution of sodium molybdate is introduced into the lower extraction stage of the extractor, and then the process of extraction (and washing of the extract) of technetium-99m is realized as described above. At the end of the process, the extract enters the evaporator and gets evaporated to dryness. The dry residue is dissolved in 0.9% NaCl solution and, after analysis, is dispensed for transfer to consumers. The subsequent cycles of technetium extraction are carried out according to the scheme above, taking into account the halflife of the parent molybdenum-99.
The first facility for the production of 99m Tc radiopharmaceuticals was placed in the Khlopin Radium Institute in a line of two hot cells and one laminar box, arranged in series.
In the first hot cell, the irradiated target was disclosed and the molybdenum trioxide was dissolved. An extractor and an evaporator were set in the second hot cell. The dissolution of the irradiated target is carried out once a week, and the extraction of technetium-99m is carried out daily. In the laminar box, the final product is dispensed in vials, sterilized and after receiving the results of quality control is sent to the clinics.
Evaluation of the cost of radiopharmaceuticals obtained with the extraction generator showed that they are much cheaper (2-5 times) than sodium pertechnetate, obtained from sorption generators. This is primarily because of the huge material costs associated with the irradiation of uranium-235 and the processing of waste generated. In the case of the use of enriched molybdenum-98, it was regenerated and re-used after processing.
To date, more than 20 years of experience have been accumulated in the operation of such a facility for the needs of nuclear medicine laboratories in St. Petersburg. The increase in the demand for technetium-99m in clinics led to the necessity of the increase in production volume and later the parallel work of the two extractors was organized.
Usually, a fresh molybdenum-99 solution is loaded into the extractor on Friday evening. At the same time, the "blank" extraction by MEK is carried out to remove all recoverable chemical and radionuclide impurities from the molybdenum-99 solution. The separation of technetium-99m from the solution of molybdenum-99 for the run-up of a series of preparations is carried out on Monday morning (after 2.5 days after "blank" extraction) and then every other day for 5 days. At the moment of molybdenum dissolution and loading into the extractor, its activity is usually 200-300 GBq (~ 6.0 -8.0 Ci). The yield of technetium-99m into the final prepackaged product is 85 to 90%, taking into account the decay at the beginning of the extraction of technetium-99m from the molybdenum-99 solution. There was no decrease in the yield of technetium-99m during the whole working week.
A study of the possible consequences of MEK radiolysis in the extractor was carried out in [24,25]. Low boiling radiolysis products are removed together with MEK during its evaporation and do not affect the quality of the finished product. The main products of radiolysis with a boiling point higher than those of MEK are its dimmers, the amount of which in the extract (with the above-mentioned 99 Mo loads) are comparable to their content in the initial reagent and practically do not affect the quality of the preparation obtained.
The product is delivered to medical institutions with in no more than 2 hours. The number of such institutions is from 5 to 15 depending on their applications. The amount of the product delivered varies from 40 to 150 GBq (1-4 Ci). According to statistics, unscheduled stops that led to the termination of deliveries throughout the workweek occurred on average 1 time in three years, which was due to various reasons: the problem with the supply of irradiated targets, the failure of auxiliary equipment, the human factor, etc. During the whole operation of the system, no series of products was rejected because of a lack of quality. The quality indices of the five series of technetium-99m preparations at the time of manufacture and at the end of its shelf life (18 hours) are presented in comparison with the chromatography generator eluate quality indicators (Table 1).
All control tests were carried out in accordance with the methods described in the State Pharmacopoeia of the Russian Federation Comparison of the characteristics of the preparations obtained on different days after molybdenum-99 solution loading shows the absence of the influence of the molybdenum-99 solution lifetime on the quality of the preparations obtained during this period. At the same time, the preparation obtained is significantly (by an order of magnitude) purer in terms of 99 Mo and other radionuclides content. The established limit of MEK content is 20 times lower than that adopted in the United States Pharmacopeia. This technology allows varying the volumetric activity of the product changing the volume of sodium chloride solution used to dissolve technetium-99m. Usually, at the request of consumers, the volumetric activity of the final product is closer to the upper limit of the permissible values. Thus, the long experience of the extraction generator has shown its high efficiency and the promise of the technical solutions contained in it.
The developed apparatus allows obtaining steadily the product of proper quality.

Automation the of Technetium-99m Extraction Process
After successful tests of centrifugal extractors in the facilities for the production of radionuclide's used in nuclear medicine ( 99m Tc [24], 188 Re [25,27,28], 90 Y [5]), extraction technology was commercially develop at Medradiopreparat Plant (FSUE "FederalСenter of Designing and Development of nuclear medicine objects" of FMBA of Russia) for the serial production of sodium [ 99m Tc] pertechnetate and supplies to clinics in Moscow. To master the technology, a semi-automatic installation was developed. The installation included a centrifugal extractor, a preparation module and a remote control panel ( Figure 4). The operations were controlled by electromagnetic valves, peristaltic pumps and a vacuum line. The extractor and the preparation module were placed in a two-section radiochemical box and the control unit was located in the operator zone. Glass elements, fittings and pipes were provided only by industrial manufacturers and allowed for use in pharmaceuticals. A thermo -and cryostat for the evaporation process were built into the system. All operations are carried out in a semi-automatic mode, including washing process and the drug preparation process. Control of the working valves is carried out from the touch screen using a program designed specifically for sodium [ 99m Tc] pertechnetate preparation. Ready solutions of initial reagents are fed     into the chamber just before production starts. The duration of the process is 40 minutes.
There are two modes of equipment operations: Manual mode: The operations in the auxiliary stage of work and the main technological process are carried out by pressing the on-screen display buttons.
Auto mode: The operations in the auxiliary stage of work and the main technological process are carried out according to the algorithm in the control program. The operator monitors the performed process confirming the execution of the main operations.
The module is intended for operation in radiochemical laboratories of medical institutions or in the RP producing facilities. A demonstration version of the installation (the extractor is located behind the frame on which the vessels for the initial reagents, the evaporator, and the receiver of the product are mounted) and the screen of the display panel figure 5.
In working condition, the initial reagents and extractor are placed in a separate section of the hot cell.
The software allows to: • set the operating modes of the module and promptly make changes to the mode from the touch screen display • control the execution of the process occurring in the module according to the mnemonic scheme and the information displayed on the screen in real time • control the operation time of the replaceable module elements, exposed to radiation and aggressive environments • There are the functions of the service mode, which allow to search and localize the malfunction of the module elements Since 2010, the semi-automatic synthesis module has been put into serial operation. 99 Mo, obtained by thermal neutrons irradiation of molybdenum oxide enriched in 98 Mo in a nuclear reactor was used as the raw material. The initial radioactive raw material was 99 MoO 3 , sealed in quartz ampoules. As a rule, the activity on the date of certification was 300-400 GBq, the specific activity was 75-100 GBq/g. Ampoules were opened with a special device, then the contents were dissolved in a mixture of 5 M KOH and K 2 CO 3 solutions with heating (70-90° C, dissolution time is 15 min). In the resulting 99 Mo solution sample (0.2 ml), volume activity was monitored (should be at least 1 GBq/ml on the day of the generator charging) and 99 Mo authenticity. The work of the extractor and evaporator is realized in an automatic mode. The process is finished by dispensing and packing of the radiopharmaceutical in the vials with subsequent sterilization in an autoclave according to the rules for the production of parenteral drugs. The technological yield of the product by activity is not less than 95% at the date and time of production. The product quality indexes fully corresponded to those presented earlier ( Table 1). The requirements to the radiopharmaceutical quality of the sodium pertechnetate solution are presented in the line 1 of table 1. The methods for quality determination are fully described in European, United States or other National pharmacopoeia. For determination of radiochemical purity we proposed to use thin-layer chromatography in acetone as mobile phase except descending paper chromatography in water -methanol (20:80 v/v) with development time for about 2 h. Time of chromatography is about 15 min. In this system pertechnetate ions migrate with solvent front.
It was established that when using raw materials with the activity of 200 GBq or higher under conditions of irregular operation of the extractor, it is possible to observe the radiolysis of the extractant, which is accompanied by the production of colored solutions of the product and extractant. However, with daily production of the radiopharmaceutical and regular maintenance of the equipment (complete release of traces of the used reagents and washing once a week), it is possible to load up to 350-400 GBq of raw material. Thus, the main criterion influencing the radiochemical purity of sodium [ 99m Tc] pertechnetate is not the total activity of the feed, but the regularity in production and maintenance of the setup.

Extraction Release of 99m Tc from 99 Mo Obtained by the Photonuclear Reaction
Alikhanyan National Science Laboratory (Yerevan Physics Institute) was founded in 1943 for investigation of the high energy physics and cosmic rays. Firstly it was based on two high altitude cosmic ray stations (~2000 and 3000 m s.l.a.). In 1967 the ring synchrotron with 4.5 GeV energy of electrons has been installed. Till 2000 a lot of fundamental investigation has been done using this accelerator. Besides ring accelerator a few small linear accelerators are working for applied research, technology development and other areas. In particular the injector of ring accelerator is powerful enough with the energy up to E e =75 MeV ( Figure 6).
It was used as a source of intensive electron beam for 99 Mo/ 99m Tc and 123 I production technology development.
One of alternative methods of 99m Tc production is a photonuclear reaction. 99m Tc could be obtained in the photonuclear reaction by irradiation of 100 Mo under intensive photon beam ( Figure 6). For this aim the electron beam should be converted to a photon beam via bremsstrahlung. This method cannot provide high specific activity and therefore does not permit the production of 99 Mo/ 99m Tc generators  with standard activity but could cover the demand for regional and city clinics.
To use its electron beam for photon-induced reactions the electron gun had to be upgraded. A new high intensity metallic cathode was installed with slightly modified gun electrodes so that the maximum intensity was increased from 3 µA to ~10 µA. From 3 accelerator sections 2 were used providing E e = 40 MeV electron energy. The electron beam was transported to the target. Magnetic optics provided the beam spot diameter on the target equal to 12 mm. The beam profile was measured by luminofoor frame or vibrating wire scanner. The beam pulse length was ~1.1 µsec, repetition frequency f=50 Hz.
A special experimental setup has been designed and mounted for material irradiation that provides remote controlled motion of the target module across the beam direction adjusting the center of the target to the beam axis (Figure 7).
The target housing module was fabricated of copper. A thick tantalum plate has been installed on the entrance window to convert the electron beam to photons. A Monte-Carlo simulation of an optimal thickness of the converter has been performed. The optimum thickness of the tantalum radiator is about 2 mm (Figure 8).
The oxide of natural molybdenum МоО 3 was used for the irradiation. The abundance of the stable isotope 100 Мо/ nat Mo is 9.63%. The irradiated material was packed in a special aluminum capsule. Two styles of target materials were used -a stack of pressed pellets and full length pressed powder covered by thin copper foil with 0.045 g/cm 2 areal density.
For the low specific activity option only direct extraction of 99m Tc from the irradiated material is a reasonable option. For that, a centrifuge extractor with MEK solvent technology was chosen. The complete semi-automated system commissioned from "Medradiopreparat" was installed in a hot cell. The natural MoO 3 target of 20 g mass and areal density 0.8 g/cm 2 has been irradiated under electron beam with energy E e = 40 MeV and average intensity i.e., ~ 9.5 µA for a duration of T=100 hours. The irradiated material was then processed by the centrifuge extractor and the first trial amount of 99m Tc has been produced. The decay correction to the EOB (End of Bombardment) yielded ~ 80 mCi (~ 3 GBq).The normalized specific activity was 3000 Bq/mg·µA·h, which is close to the maximum value of the previously published results [14].

Тс Radiopharmaceuticals
In accordance with the national requirements for the conditions and procedures for the use of new developments in clinical practice, sodium [ 99m Tc] pertechnetate preparations have been tested for suitability for the manufacturing of other 99m Tc RPs based on domestic kits to the 99 Мо/ 99m Тс generator. This procedure was carried out every time when a new production site was organized and included the following mandatory studies: • Determination of the main quality indices of the RPs produced using eluates from extraction and chromatographic generators -volumetric activity, Radiochemical Purity (RCP) or Radiochemical Impurities (RCI) and pH • A comparative study of the biological distribution of prepared RPs at the most critical time after intravenous administration, which were previously identified in the report on preclinical studies of a radiopharmaceutical approved for clinical use Tested RPs were prepared by dissolving of lyophilized kits in sodium [ 99m Tc] -pertechnetate solutions obtained from extraction generator (Facility "Medradiopreparat", Moscow, Russia) or generator of chromatographic type (GT-4K, FSUE "Karpov Institute of Physical Chemistry", Obninsk, Russia). Quality control of tested RPs (RCP or PCI) was carried out by thin-layer chromatography or descending paper chromatography in different solvents or electrophoreses in agarose gel according to the national monographs.
Since the results obtained in the tests were almost the same, the summary data of such studies are selectively (for the most commonly used RPs) presented in table 2. No difference in labeling efficiency, quality and biological behavior of the radiopharmaceuticals (within the limits of acceptable errors) was observed.

Conclusion
The centrifugal semi-countercurrent extractor has proven itself as effective, reliable, easy-to-use equipment capable of stable production of a product -sodium [ 99m Tc] pertechnetate solution of proper quality.
Currently, the development of a new synthesis module is in progress. The main differences between the future synthesis modules are its mass-dimensional parameters (Figure 9). Due to the use of new design solutions it was possible to abandon the use of cryo -thermostats included in the current set of equipment. By using compact valves and tubes, the dead zones volume is reduced.
The software of the system is implemented on the base of a computer. To ensure greater operational safety, the application interface is organized in such a way that only necessary and meaningful system settings are available to the operator at each process operation. Along with a completely new interface, the software of the complex includes the following functions: • Logging all operator actions • Privileges for different types of users are established (separately for personnel engaged in daily production or maintenance of the installation)   Although the set of equipment is self-contained and is no significantly expand of its capabilities is expected, the platform is built in such a way that adding support for new features at the customer's request will not lead to drastic changes (and will not take much time). Among such features can be, for example, duplication of the log file in the factory database (SQL, Oracle), switching to another hardware complex (transfer of software to another hardware platform), and integration into SCADA -the customer's system. It is possible to transfer the software to other platforms running Windows/Linux. Also, there is no problem with the integration of the modern element base (the control PC can be extended with both analog signals and digital ports).
The developed technology and technical devices should be considered promising for the organization of local technetium-99m     The results of a comparative study of the quality parameters of RPs prepared using domestic-produced kits (Diamed, Moscow, Russia). *) first line for every RP -the results obtained using sodium pertechnetate solution from chromatographic generator (GT-4K, FSUE "Karpov Institute of Physical Chemistry", Obninsk, Russia) and served as a reference source; The second line of every RP -the results obtained using sodium pertechnetate solution from extraction generator (FSUE "Federal center of nuclear medicine projects design and development", Moscow, Russia); **) time after intravenous injection specified in the instruction for medical use of each radiopharmaceutical  production facilities, including specialized radiochemical laboratories for medium-capacity medical cyclotrons. In addition, it is possible to organize the simultaneous production of small batches of ready-touse radiopharmaceuticals with subsequent delivery to radionuclide diagnostic units. This provides an opportunity to guarantee the receipt of high-quality RPs and reduce of the radiation doses obtained by medical personnel.