HSOA of Atmospheric & Earth Sciences Study of Physico-Chemical Characteristics of Water and Soil in Relations to Fish Production in Motia Lake Reservoir

Study of Physico-Chem- ical Characteristics of Water and Soil in Relations to Fish Production in Motia Lake Reservoir. J Atmos Earth Sci 2: 006. Abstract Present studies were carried out for physico-chemical parameters of water and soil of Motia Lake Bhopal, Madhya Pradesh, India. The aim of the study was to establish the safety of water and fishes from the Lake. Different physico-chemical parameters such as pH, Electrical Conductivity (EC), Dissolved Oxygen (DO), Total Alkalinity (TA), Total Hardness (TH), Chloride (Cl), Nitrates (NO 3 ), Phosphate (PO 4 ), Turbidity and Total Dissolved Solids (TDS) of lake water were investigated during pre-monsoon, monsoon and post-monsoon period. The soil parameters such as pH, EC, phosphorus, nitrogen, potassium and organic matter were also studied in different sessions. The value of pH, EC, DO, TH, TA, Ca, Mg, Cl, NO 3 , PO 4 were recorded higher during pre-monsoon period in lake water. In soil sample, pH, EC, nitrogen was found higher in post-monsoon during study period. The results on physico-chemical parameters of water and soil clearly indicate highly productive nature of the Motia Lake. The water quality parameters showed maximum values during pre-mon-soon compared to rainy and winter seasons. It was inferred from the results of water quality and soil analysis that Motia lake was produc- tive in nature.


Introduction
India's inland water bodies are as diverse as they are plentiful. These are important source of food and also provide employment for the people of rural India. India is the second largest producer of Inland fishes. At present, about 80% of inland fish produced in India is derived from aquaculture and 20% from capture fisheries. In India water reservoirs have a rich and varied spectrum exceeding about 400 species. These made ecosystem offer enough scope for stock manipulation through ecological maneuverings paving the way for production hikes at relatively low capital investment. Majority of reservoirs in our country are not being scientifically managed, only some are half heartedly managed or sometime even not managed too.
In limnological study the consideration of physico-chemical factors contribute in making up of the specific ecosystems, which determined the trophic dynamics of the water body. It is necessary to know the physico-chemical properties of water to study the culture practices of the fish in water bodies. The productivity of a water sheets depends primarily on the physico-chemical and biological characteristics of water. These properties again depend on the nature of bottom soil and climatic conditions. In India first ecological study on a reservoir was carried by Ganapathi on Red hill Lake, Madras [1].
Soil is one of the most important ecological factors containing a large number of different kinds of bacteria, plants and animals that compose inter-related biological complex. As a complex physical and biological system, it provides nutrients and oxygen for the organisms. The major physical and chemical features of soil are related to particle size, distribution, types of clay colloids, cation and anion exchange capacity, oxidation and reduction potential, soil reaction, electrical conductivity and nutrient dynamics. A healthy and judicious reduction condition should be maintained at the bottom to facilitate the optimum availability of nutrients as well as prevention of any unhealthy situation. Thus, soil plays an important role in fertility of fish life and water body.
The physico-chemical parameters of water like temperature, pH, electric conductivity, dissolved oxygen, biological oxygen demand, chemical oxygen demand, total alkalinity, carbonates, bicarbonates, calcium, magnesium, hardness, chlorides, sulphates, nutrients like nitrates and phosphates, turbidity and total dissolved solids are important to know the trophic nature of the water body. Water bodies are generally of three types oligotrophic, mesotrophic and eutrophic. Usually mesotrophic water bodies are highly productive in nature.
In this study a perennial small water reservoir known as Motia lake, which is the most disturbing reservoir is used for different

Materials and Methods
The present study was conducted in a minor reservoir Motia lake in the center of capital city of Bhopal, Madhya Pradesh, India (23° 16' N & 77° 36' E; 550 meters above MSL) spreading over 7 hills (Figures 1 and 2). The water spread area is 10.89 hectares. The age of the reservoir is 90 years. The reservoir is located in Bhopal city with a well built tank bund. The reservoir water is used for many purposes including fishing activities. Bhopal city is called the city of lakes. The two main lakes upper lake and lower lake provide livelihood and add to scenic beauty of Bhopal city. This is endowed with a number of eighteen water bodies developed over a period of 900 years ( Figures  3 and 4).
The studies were conducted on physico-chemical parameters of water and soils in relation to fish production. The following physico-chemical parameters of water were analyzed to know the status of reservoir and its impact on fish production. The parameters were hydrogen ion concentration (pH), Dissolved Oxygen (DO), carbonates, bicarbonates, total alkalinity, calcium, magnesium, nitrates, phosphates, chlorides, sulphates, turbidity and dissolved solids. These parameters were analyzed thrice in a month for two years in selected stations of the reservoir. The average value was obtained for each month. The soil parameter such as pH, Electrical Conductivity (EC), nitrogen, phosphorous, potassium and organic matter were also analyzed.

Sampling Procedure
The water samples were collected in five stations of the reservoir and one liter of water was collected in a wide mouth polythene bottle and tightly stopper for subsequent physico-chemical analysis. Another bottle of the same capacity was similarly filled with surface water for biological analysis. Soil sample was collected from the same stations at a depth of one meter water level into a polythene container for the physico-chemical analysis. The methods adopted for the analysis of physico-chemical parameters are briefly described below.    Hydrogen ion concentration with the help of pH meter; Turbidity at 420 nm by using formazine polymer as turbidity standard for reference and expressed in formazine units; Total Solids (TS) estimated by evaporation of 50 cm 3 aliquot at 105°C and desiccation of the revolve to constant weight, finally calculated as mg/cm 3 ; Dissolved Solids (DS) a part of the sample was filtered through Whatman No 1 filter paper and 50 cm 3 aliquot of filtrate was evaporated at 105°C and desiccated to constant weight and finally calculated as ppm; Dissolved Oxygen (DO) was fixed in the field by winklerization and analyzed by titrimetry and finally expressed as mg/cm 3 at ambient temperature; Carbonates and bicarbonates were estimated in 50 cm 3 aliquot by acidimetry using phenolphthalein and methyl orange as indicators; Nitrate nitrogen estimated by phenol disulphonic acid method; Calcium determined by EDTA titration method using murexide as indicator; Magnesium measured by EDTA titration method using eriochrome/ solochrome black as an indicator; Phosphates measured by EDTA titration method and dissolved chloride content was estimated by argentimetry using potassium chromate as an indicator [2,3].
All the physico-chemical analysis were completed within 48 hours after the collection of the samples and all stipulated precautions were followed during the collection, storage, handling and processing so that the precision and accuracy were within the ranges prescribed by Apha, Golterman and Neeri [2,4,5].

Physico-Chemical parameters of water
Study on various physico-chemical parameters in relation to fish production were analyzed in Motia lake, 2011-2013, which are as follow: Hydrogen ion concentration (pH): The pH of water has significant role in survival of aquatic biota. The pH ranged from 7.8 to 8.  Table 1).
The functional stability of aquatic ecosystem depends upon the buffering capacity of water. This is reflected from the fluctuation of the hydrogen ion concentration brought about by the operational dynamics of CO 2 , bicarbonates and carbonates. Most of the fresh water bodies are alkaline due to the above components. The productivity of inland water is good in a slight alkaline reaction with pH between 7.5 to 8.5 [6][7][8][9].
The alkaline pH provides the growth to natural food organisms and increases the productivity and fish yield. Similar reports were made by Piska et al., [6,7,9,10]. Malu reported that the higher pH can be attribute to higher primary productivity and attributed to high rate of photosynthetic activity [11]. Das and Nandi observed that pH between 6.5 and 9.0 supports a good fishery [12]. It has direct effect on fish growth as well as on the growth and survival of fish food organisms.
pH is the most important factor in determining the reservoir productivity. The water pH is more or less a reflection of bottom soil. It has been revealed that less than 5 and more than 10 pH was lethal to fish. pH of water undergoes a diurnal change alkaline medium. In slight alkaline pH (7-8 pH) water was observed more suitable for fish life [12].

Electric Conductivity (EC):
The conductivity ranged from 1180 µS/cm to 1470 µS/cm and 1240 µS/cm to 1420 µS/cm was recorded during 2011-2012 and 2012-2013 respectively. The maximum conductivity was noticed in pre-monsoon period and minimum was in monsoon period (Table 1). It has been observed that EC between 20 and 1500 μs/cm is suitable for aquaculture .Higher value of EC occurred due to the decomposition of macrophytes, dead animal and evaporation.
The conductivity of water depends on ions present in the water. The conductivity reflects the nutrient status of the water and distribution of macrophytes. Minimum conductivity may be due to dilution of water, caused by monsoon rains and utilization of ions by the living community of the reservoir. Higher values of conductivity was recorded, may be due to decomposition of macrophytes, dead animals present in the reservoir, evaporation and evapo-transpiration of the reservoir. Electrical conductivity has significant positive correlation with free carbon dioxide, bicarbonate, alkalinity, potassium and phosphate and negative correlation with dissolved oxygen carbonate alkalinity and nitrates.   Unni et al., has reported that seasonal variation of conductivity showed maximum (4949 µS/cm) in summer of Tawa reservoir, Madhya Pradesh. The input of nutrient ions through thermal power effluents and its subsequent distribution and dilution resulted in the longitudinal decline of conductivity in the reservoir [13]. Das has studied the limno-chemistry of some important reservoirs of Andhra Pradesh and observed specific conductivity in the range of 316 to 610 µS/cm. The majority of Indian reservoirs have low values of specific conductivity [14].  (Table 1), with over all mean of 5.96 ppm.
The lower values of dissolved oxygen in monsoon season and higher values in pre-monsoon were due to the surfacial water of the reservoir during monsoon months, subjected to wind generated turbulence and resultant mixing of surface and surface water layers. Thus, in monsoon months, there establish oxygen equilibrium between the water and air. This is not disturbed by the vertical gradient of phytoplankton's or bacterial populations.
The pollution stress is increased in water bodies due to the enrichment of nutrients and decline of dissolved oxygen. The presence of pollutants like organic wastes caused rapid depletion of dissolved oxygen. Substances like NH 3 , nitrates, H 2 S and oxidisable inorganic substances also decrease the dissolved oxygen in water [15].
Das studied the limino-chemistry of important reservoirs of Andhra Pradesh and stated that dissolved oxygen along with turbidity could provide information about the nature of an ecosystem better than any other chemical parameters [16]. It has been observed that dissolved oxygen concentration more than 5 mg/l favors good growth of flora and fauna. Motia lake being a polluted and highly eutrophic reservoir showed higher dissolved oxygen in pre-monsoon due to algal bloom.
Total alkalinity: Present study revealed that the concentrations of total alkalinity were observed higher in the pre-monsoon season  Table 1). The overall mean concentration was found to be 121.3 mg/cm 3 during the study period.
The alkalinity of the water is mainly due to carbonates, bicarbonates and partially hydroxides. Carbonates and bicarbonates along with free CO 2 and carbonic acid form four species of inorganic carbon of the fresh water carbonic system. Their interaction with water molecules results in the displacement of H + and OHions and manifestation of a specific pH at which, the relative concentration of H 2 CO 3 , HCO 3 and CO 3 2were fixed [6,7,17].
Yelavarthy has studied the hydrobiology of Red Hills reservoir, Tamil Nadu and noticed the total alkalinity in the range from 117.3 to 173.3 mg/cm 3 and shows a close correlation with productivity [18]. Rao reported the total alkalinity ranged from 112 to 22 mg/cm 3 in Julur [19].
Total hardness: In the present study, the minimum total hardness was recorded in the monsoon (198 mg/cm 3 Table 1).
The total hardness of water is caused by Ca and Mg ions present in water. Hardness could be temporary due to carbonates and bicarbonates or permanent due to sulphates and chlorides. Biologically temporary hardness plays a key role in buffering capacity, thus neutralizing the pH due to addition of acidic products. It has a great effect on biotic diversity of an ecosystem. Maximum values of the calcium and magnesium were found during pre-monsoon months (94 ppm; 101 ppm and 45.9 mg/cm 3 (Tables 1 and 2).  The relative composition and concentration of the Cations in the aquatic ecosystem determines the water quality for different uses. The hardness is due to calcium and magnesium, which are bivalent ions. Calcium and magnesium cycle through biotic and abiotic components of the ecosystem. As a result ambient concentration is under the influence of the photosynthetic precipitation, biotic utilization and tropholyte release. Higher hardness values were recorded during summer.

S No Season year 2011-2012 Turbidity (NTU) Cl (mg/cm 3 ) NO 3 (mg/cm 3 ) PO 4 (mg/cm 3 )
The lower values of calcium were found during winter and magnesium in monsoon months [20]. Swingle has suggested that total hardness of 50 mg/cm 3 CaCO 3 equivalent was the dividing line between soft and hard water [21]. He reported that the pond water having a hardness of 15 mg/cm 3 or above were satisfactory for growth of fish and do not require additional of lime. Similar observations were made by Piska et al., [9].

Chlorides:
The chloride content was found high during pre-monsoon months and low during monsoon months. The ranges of chlorides were found to be 228 mg/cm 3 to 345 mg/cm 3 and 241 mg/cm 3 to 371 mg/cm 3 during the study period (Table 2).
Natural water normally has a low chloride content compared to bicarbonates and sulphates. High chlorides indicate pollution from domestic sewage and industrial effluents. Chloride content above 250 mg/cm 3 makes water salty [22].
Das has studied the limno-chemistry of Andhra Pradesh reservoirs and reported that Krishna reservoirs showed significantly higher values of chloride content as compared to Godavari and Penna basin reservoirs due to inherent characteristics of its catchment [23].
Pulle and Khan reported that chloride content was found to be 32.2 to 48.7 mg/cm 3 in Isapur dam water of Maharashtra and the presence of chloride indicates the availability of organic matter, presumably of animal origin and increased the amount of ammoniacal nitrogen and organic matter [22]. The concentration of chloride increases with the degree of eutrophication. Yelavarthy has reported the low chloride levels of Red Hills reservoir, Tamil Nadu [18]. Rao reported high values of chlorides in Safilguda reservoir [24].
Nitrates: Maximum nitrates were found during pre monsoon months in both cases. The highest value of nitrates were (35.8 mg/cm 3  Nitrate nitrogen in water of Indian reservoirs was found mostly in traces and seldom exceeds 0.5 mg/l [23]. Unni et al., and Mohanty reported the nitrogen levels of various reservoirs of India and discussed its impact on productivity [13,25]. Das has studied the concentration of nitrate nitrogen in nine important reservoirs of Krishna, Godavari and Penna river system and reported that in all the reservoirs the concentration of nitrate is rather poor in traces [23]. However, the level of nitrate was improved during post-monsoon months to the extent of 0.56 mg/cm 3 (Kadam) to 0.86 ppm (Nagarjuna Sagar). The major reservoirs have very low concentration of nitrogen and phosphorous (0.56 ppm) [26]. The highly productive reservoir with nitrate range of 0.75 to 1.65 mg/cm 3 was noticed.

Phosphate:
The values of phosphate were ranged from 5.10 ppm to 8.58 ppm during 2011-2012 and 5.00 mg/cm 3 to 8.27 mg/cm 3 during 2012-2013 (Table 2). High values of phosphates during pre monsoon were due to reduced water level.
During monsoon period high values of phosphate were recorded. It was contributed by the surface run off and mixing with the influent water of the reservoir. Piska in Ibrahimbagh and Shathamraj reservoirs of Ranga Reddy district were also reported similar results [6,7]. The present study also coincides with the findings of Yound et al., and Piska [10,27]. Rao et al., reported an increase in the phosphates concentration in the waters polluted by sewage [28].
In the present observation the concentration ranges of the phosphates were agreeable with the opinion of Jhingran and Sugunan which were 7.235 ppm and indicate that the reservoir is under highly productive in nature [29]. Ecologically phosphorous is often considered as the most critical single element in the maintenance of aquatic productivity. Large number of lakes and ponds gave the phosphorous fertility range as 0.00-0.02 mg/cm 3 (low productivity) 0.02-0.05 mg/ cm 3 (fair productivity) 0.05-0.10 mg/cm 3 (good productivity above 0.2 mg/cm 3 as excessive [18]. Water turbidity is mainly due to suspended inorganic substances like clay, silt, phytoplankton and zooplankton and sand grains [30]. Reservoirs with clay bottom are likely to have high turbidity. Turbidity reduces the sunlight penetration and photosynthesis, hence acts as a limiting actor. High turbidity reduces the dissolved oxygen in water. In less turbidity waters, the aquatic weed growth is more. In high turbid waters, the sand grains accumulate in the gills of fish and prawns, causing suffocation and excessive secretion of mucous.
In turbid waters, more light is scattered and absorbed rather than transmitted in straight lines. The light penetrates only to shallow depths. The lower layer of water, being devoid of photosynthetic plants and also being in close contact with the decaying organic matter, suffers from oxygen depletion, causing the death of fishes [6,7].

Total dissolved solids:
The total dissolved solids were maximum during pre-monsoon season during entire study period. These values were lowest during monsoon period. Highest values of total dissolved solids were 860 and 890 mg/cm 3 during 2011-2012 and 2012-2013 respectively (Table 1).
During the present study, minimum amount of dissolved solids have been observed in pre-monsoon when the water level comes down considerably due to evaporation. The increase in the concentration could be directly related to the surface area of the reservoir. Large amounts were due to run off water from attachment area. Aher et al., reported that seasonal change in water quality parameters of Kagdipura swamp near Aurangabad were high due to the sewage [31]. Salve and Hiware studied the water quality parameters of Wamparakalpa reservoir Nagpur and reported alkaline nature of water and useful for drinking [32]. Rajashekar et al., and Rao studied the water quality parameters of Nadergul reservoir and reported mesotrophic nature and water in useful for irrigation and fishing activities [19,33].  (Table 3).

Physico-Chemical Parameters of Soil
According to Jhingran and Sugunan, the soil pH 6.5 to 7.5 was medium productive, more than 7.5 pH was more productive and less than 6.5 pH was low productive in reservoirs of present study [29].
The pH values were around 7.5 hence the present reservoir is high productive in nature. The Ministry of agriculture has reported that a slightly alkaline soil with pH 7.5 was considered optimal for fish production in reservoirs.

Electrical conductivity:
Electrical conductivity values were ranged between 1530 to 1620 µs/cm -1 and 1540 to1610 µs/cm -1 during 2011-2012 and 2012-2013 respectively. The fluctuations were negligible. Decrease in conductivity during the winter may be due to utilization of ionic minerals by the producer community (Table 3).
Das reported that the changes in Electrical Conductivity (EC) were associated with the release or depletion of soluble ions in the soil water system [14]. It definitely might have an indirect role to play in reservoir productivity. Specific conductivity (mmhos/cm) is very low in the reservoir under Cauvery (0.19-0.34) Godavari (0.42-0.72), Krishna (0.56-0.86) and Penna river (0.51-0.60) basins.
Phosphorus: Phosphorus content in soil was ranged from 6.9 to 6.4 mg/100 g and 3.2 to 5.9 mg/100 g during 2011-2012 and 2012-2013 respectively. The fluctuations were negligible and no particular trend was observed in both the years (Table 3).
According to Mahajan and Mandloi available phosphorous in the soil of fish cultured pond of Madhya Pradesh was maximum in rainy season and minimum in summer [34]. Same was reported by Nath in Sardar Sarovar dam [35]. Das stated that soil available phosphorus should be in the range of 4.7 to 6.2 mg/100g for productivity [23]. Soil is highly alkaline under The Krishna and The Penna river systems as also depicted by lesser quantity of available phosphorus in those reservoirs.  Table 3).

Nitrogen
The range of available nitrogen 50-75 mg/100 g of soil is relatively more favorable for reservoir productivity [36]. Das observed that deep reservoir contain lesser amount of available nitrogen (Srisailam, 7 m deep, 27 mg/ 100 g nitrogen) than shallower reservoir (Wyra 5.5 m deep, 63 mg/100 g) [23]. Nitrogen concentration in the soil of Adhartal pond, Madhya Pradesh was high in post monsoon period (10-65 to 10-80 mg/100 g) and low in pre-monsoon (0.78 mg/100 g) due to the rain water might have washed additional nutrients such as nitrate and phosphate from surrounding area [34]. A significant part of utilized nitrogen is also lost in the sediment and alters the soil nutrient status and benthic community structure [37]. The values were high in post monsoon period and low in pre-monsoon period during the two years. In general the potassium values were high in the soil of present reservoir (Table 3). According to Murugesan the low level of K + was reported in the soil and water of Tamil Nadu reservoirs (<3.00 mg/100 g -3.12 mg/ 100 g soil) [38,39].