Antimicrobial Effect of Secondary Metabolites Produced by Some Bacillus Strains Developed in Different Energy Sources

Abstract

In this study, it was aimed to determine antimicrobial activity properties of Bacillus sp. isolates in agricultural land in Kahramanmaraş. Bacillus isolation was done from soil samples collected agricultural land. These isolates were described as morphologically and biochemically. The antimicrobial activities in different carbon sources (glucose, fructose and saccharose) of the Bacillus isolates and reference Bacillus species against some bacteria and pathogenic yeast (Candida albicans) were examined by the disk diffusion method. At the result of the research, secondary metabolites of Bacillus isolates used in the study inhibited the growth of tested microorganisms in varying ratios (1-25,2 mm zone of inhibition). Three isolates (Bacillus sp. B6, B13 and B43) showed high antibacterial activity. It was found that Bacillus sp. B6, Bacillus sp. B13 and Bacillus sp. B43 produced antifungal secondary metabolites against Candida albicans at all used carbon sources (except Bacillus sp. B6- saccharose). More importantly, it was observed that Bacillus sp. B13 (1-11,67 mm zone of inhibition) and Bacillus sp. B43 (1,4-19 mm zone of inhibition) has antibacterial and antifungal effect against all pathogenic microorganisms when fructose is used as the carbon source.