A HYBRID CAPTURE BAIT SET FOR BEGONIA

Authors

  • T. Michel Institute of Molecular Plant Sciences, University of Edinburgh, The King’s Buildings, Edinburgh EH9 3BF, Scotland, UK./Royal Botanic Garden Edinburgh, 20A Inverleith Row, Edinburgh EH3 5LR, Scotland, UK. https://orcid.org/0000-0001-5717-5643
  • Y.-H. Tseng Research Museum and Herbarium (HAST), Biodiversity Research Center, Academia Sinica, 128 Academia Road, Section 2, Taipei 115201, Taiwan. https://orcid.org/0000-0002-8166-5690
  • H.P. Wilson Royal Botanic Garden Edinburgh, 20A Inverleith Row, Edinburgh EH3 5LR, Scotland, UK./Institute of Biodiversity Animal Health & Comparative Medicine, Glasgow University, Graham Kerr Building, Glasgow G12 8QQ, Scotland, UK. https://orcid.org/0000-0002-5231-3987
  • K.-F. Chung Research Museum and Herbarium (HAST), Biodiversity Research Center, Academia Sinica, 128 Academia Road, Section 2, Taipei 115201, Taiwan. https://orcid.org/0000-0003-3628-2567
  • D.C. Thomas 1. National Parks Board, Singapore Botanic Gardens, Research and Conservation, 1 Cluny Road, Singapore 259569, Singapore. https://orcid.org/0000-0002-1307-6042
  • C.A. Kidner Institute of Molecular Plant Sciences, University of Edinburgh, The King’s Buildings, Edinburgh EH9 3BF, Scotland, UK./Royal Botanic Garden Edinburgh, 20A Inverleith Row, Edinburgh EH3 5LR, Scotland, UK. https://orcid.org/0000-0001-6426-3000

DOI:

https://doi.org/10.24823/ejb.2022.409

Keywords:

Begoniaceae, Enrichment panel, Probe set, Target capture sequencing

Abstract

Hybrid capture with baits has proven to be a rich source of genetic data for many genera. The depth of information provided allows resolution of rapid radiations and of deep phylogenetic patterns. Retrieved data can also be used for population genetic studies and analysis of functional genetic diversity. To gain a better understanding of the evolutionary patterns across this large, diverse and fascinating genus through phylogenetics, population genetics and sequence analysis, we have designed and tested a set of 1239 baits covering low copy number and functionally annotated genes involved in shade adaptation and development and genetically linked to key traits. We demonstrate successful recovery of sequence data from species across Begonia and from fresh, silica-dried and older herbarium material. 

A diagram of capture by target

Downloads

Additional Files

Published

2022-08-18

Issue

Vol. 79 (2022): Begonia Special Issue

Section

Original Research Articles

License

Copyright (c) 2022 T. Michel, Y.-H. Tseng, H.P. Wilson, K.-F. Chung, C.A. Kidner

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Please read our Open Access, Copyright and Permissions policies for more information.