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In vitro propagation of Caralluma tuberculata and evaluation of antioxidant potential

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Abstract

Present study describes rapid in vitro propagation of Caralluma tuberculata, a traditional medicinal plant, and antioxidant potential of calli and plants extracts. The highest callus induction rate (93.3%) with maximum weight of calli 5.2 g was achieved from shoot tip explants on MS medium supplemented with 9.04 μM 2,4-D and 4.44 μM BA. The maximum shoot induction rate (71.1%) with mean number of shoots 3.66 ± 1.53 and 4.6 cm average shoot length was observed on 13.32 μM BA, 4.52 μM 2,4-D and 2.89 μM GA3 appended in MS medium. The developed shoots were best rooted in the presence of 5.07 μM IAA with 3.0 ± 0.15 roots per plantlet. The plants were successfully acclimatized under in vivo conditions. The plants and calli extracts exhibited good antioxidant activities, however, plant extract activities were more pronounced. The phenolic compounds in plant and calli extracts were 0.16% and 0.057%, respectively. While the flavonoids were 0.092% in plant and 0.039% in calli extract. Total Phenolics, flavonoids; DPPH radical scavenging activity and reducing power potential distributed among different fractions depending upon polarity of the solvent. The highest DPPH scavenging activity and reducing power was exhibited by water fractions; 4.95 mg/mL and 0.729 OD at 10 mg/mL, respectively. The micropropagation protocol can be successfully used for large-scale multiplication and conservation of germplasm of this threatened plant. Furthermore, antioxidant value describes importance of this valuable plant as food and medicine.

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Authors and Affiliations

  1. Horticulture and Floriculture Institute, Government of Punjab, Rawalpindi, Pakistan

    Riaz Ur Rehman

  2. Preston Institute of Nanoscience and Technology, Preston University, Islamabad, Pakistan

    Muhammad Fayyaz Chaudhary

  3. Department of Field Crop, Faculty of Agriculture, 06110, Diskapi-Altindag, Ankara, Turkey

    Khalid Mahmood Khawar

  4. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China

    Gang Lu

  5. Department of Pharmaceutical Sciences, COMSATS Institute of Information Technology, Abbottabad, Pakistan

    Abdul Mannan

  6. Department of Biotechnology, Quaid-i-Azam University Islamabad, 45320, Islamabad, Pakistan

    Muhammad Zia

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  1. Riaz Ur Rehman
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  2. Muhammad Fayyaz Chaudhary
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  3. Khalid Mahmood Khawar
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Rehman, R.U., Chaudhary, M.F., Khawar, K.M. et al. In vitro propagation of Caralluma tuberculata and evaluation of antioxidant potential. Biologia 69, 341–349 (2014). https://doi.org/10.2478/s11756-013-0322-z

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