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Microbial community shift with decabromodiphenyl ether (BDE 209) in sediments of the Pearl River estuary, China

  • Section Cellular and Molecular Biology
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Abstract

To compare the effect of decabromodiphenyl ether (BDE 209) on microbial community from the Pearl River estuary, the microbial community at three in situ sites and the responses of microbial community to BDE-209 stressor were investigated. Denaturing gradient gel electrophoresis analysis of 16S rRNA gene showed that microbial community at site A2 has less diversity than sites A1 and A3. Physicochemical parameters (NH4-N, salinity and SiO3-Si) could significantly impact the microbial community composition in this estuary. In laboratory-incubated experiments, results indicated high concentration of BDE 209 (100 mg/kg) could increase the microbial diversity at sites A1 and A2, whereas reduced the microbial diversity at site A3. The unweighted pair group method with arithmetic means cluster analysis and principal component analysis demonstrated that the community structure changes at sites A1 and A2 were driven by the BDE 209 concentration, whereas at site A3 they depended on the incubation time. Thirty-five days after the addition of 100 mg/kg BDE 209, Firmicutes were found to be the dominant bacteria at sites A1 and A2. These data suggest the BDE 209 may have different effects on the microbial community in the Pearl River estuary.

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Abbreviations

BDE 209:

decabromodiphenyl ether

CCA:

canonical correspondence analysis

DCA:

detrended correspondence analysis

DGGE:

denaturing gradient gel electrophoresis

log Kow :

octanol-water partition coefficient

MSM:

mineral salt medium

PAHs:

polycyclic aromatic hydrocarbons

PBDEs:

polybrominated diphenyl ethers

PCA:

principal component analysis

PRD:

the Pearl River Delta

UPGMA:

unweighted pair group method with arithmetic means

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Authors and Affiliations

  1. State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, People’s Republic of China

    Peng Wu, You-Shao Wang, Cui-Ci Sun, Fu-Lin Sun & Yu-Tu Wang

  2. Daya Bay Marine Biology Research Station, Chinese Academy of Sciences, Shenzhen, 518121, People’s Republic of China

    Peng Wu, You-Shao Wang, Cui-Ci Sun, Fu-Lin Sun & Yu-Tu Wang

Authors
  1. Peng Wu
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  2. You-Shao Wang
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  3. Cui-Ci Sun
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  4. Fu-Lin Sun
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  5. Yu-Tu Wang
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Correspondence to You-Shao Wang.

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Electronic supplementary material. The online version of this article (DOI: 10.2478/s11756-013-0227-x) contains supplementary material, which is available to authorized users.

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Wu, P., Wang, YS., Sun, CC. et al. Microbial community shift with decabromodiphenyl ether (BDE 209) in sediments of the Pearl River estuary, China. Biologia 68, 788–796 (2013). https://doi.org/10.2478/s11756-013-0227-x

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  • DOI: https://doi.org/10.2478/s11756-013-0227-x

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