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Genetic diversity analysis in disjunct populations of Rhododendron arboreum from the temperate and tropical forests of Indian subcontinent corroborate Satpura hypothesis of species migration

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Abstract

Rhododendron arboreum of the family Ericaceae represents one of the ancient relict tree species of the genus showing extreme disjunction in the Indian subcontinent. It is represented by two sub-species viz., ssp. arboreum Smith in the forests of north-eastern India (temperate) and spp. nilagiricum (Zenker) Tagg distributed in the southern Western Ghats (tropical) of India with apparently no distribution in the intervening plains. 35 Samples corresponding to different locations in the tropical montane forests of Nilgiris and temperate forests of northeast India were analyzed for distribution of genetic variation using 25 random primers. Relatively high genetic diversity was measured in the temperate populations (Ht = 0.21; Nm = 1.13) than tropical Rhododendrons. The hierarchical analysis of molecular variance showed that among the total variance, 25% is residing within populations while 63% of variance is among regions apparently revealing that disjunct distribution has structured genetic differentiation pattern in this species. Grouping of samples was in conformity with their spatial distribution, which was confirmed by UPGMA cluster analysis and PCA scatter plot. The taxon with its unique distribution pattern and wide cross compatibility between sub-species is however threatened by low genetic variation and gene flow that catalyses it’s shifting towards genetic drift and hence long-term conservation strategies need to be formulated particularly for the tropical Rhododendron sub-species. Among the various hypotheses and theory proposed to explain the Malayan affinity in the flora and fauna of Western Ghats, Satpura hypothesis based on dispersal model of distribution largely explains the discontinues distribution of R. arboreum.

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Abbreviations

NEI:

North-East India

SWG:

outhern Western Ghats

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Author notes

  1. Reji J. Varghese & Soorimuthu Seeni

    Present address: Department of Biotechnology Sathyabama University, Chennai, 600119, India

Authors and Affiliations

  1. Biotechnology and Bioinformatics Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute Palode, Thiruvananthapuram, 695562, Kerala, India

    Manikantan Kuttapetty, Padmesh P. Pillai, Reji J. Varghese & Soorimuthu Seeni

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  1. Manikantan Kuttapetty
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  2. Padmesh P. Pillai
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Correspondence to Padmesh P. Pillai.

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Kuttapetty, M., Pillai, P.P., Varghese, R.J. et al. Genetic diversity analysis in disjunct populations of Rhododendron arboreum from the temperate and tropical forests of Indian subcontinent corroborate Satpura hypothesis of species migration. Biologia 69, 311–322 (2014). https://doi.org/10.2478/s11756-013-0316-x

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