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Diversity and PAH growth abilities of bacterial strains isolated from a contaminated soil in Slovakia

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Abstract

Different abandoned industrial areas contaminated by polycyclic aromatic hydrocarbons (PAHs) are present in Slovakia. These environmental burdens are very dangerous to the health of human and environment. The bioremediation, based on the use of hydrocarbons degrading microorganisms, is a promising strategy to sanitize these polluted sites. The aim of this investigation was to assess the bacterial diversity of a PAHs-contaminated soil and to select the potential hydrocarbonoclastic bacteria which can be used for different bioremediation approaches. The bacterial strains were isolated on minimal medium agar supplemented with a mixture of PAHs. Seventy-three isolated strains were grouped by ribosomal interspacer analysis in 15 different clusters and representatives of each cluster were identified by 16S rRNA sequencing. The PAHs degradation abilities of all bacterial isolates were estimated by the 2,6-dichlorophenol indophenol assay and by their growth on minimal broth amended with a mixture of PAHs. Different kinds of strains, members of the genus Pseudomonas, Enterobacter, Bacillus, Arthrobacter, Acinetobacter and Sphingomonas, were isolated from the contaminated soil. Four isolates (Pseudomonas putida, Arthrobacter oxydans, Sphingomonas sp. and S. paucimobilis) showed promising PAHs-degrading abilities and therefore their possible employing in bioremediation strategies.

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Abbreviations

PAH:

polycyclic aromatic hydrocarbon

RISA:

ribosomal interspacer analysis

2,6-DCPIP:

2,6-dichlorophenol indophenol

MM:

minimal medium

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Authors and Affiliations

  1. Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, SK-84551, Bratislava, Slovakia

    Andrea Puškárová, Mária Bučková, Katarína Chovanová, Jana Harichová, Edita Karelová, Jana Godočíková, Bystrík Polek, Peter Ferianc & Domenico Pangallo

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  1. Andrea Puškárová
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  2. Mária Bučková
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  3. Katarína Chovanová
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  4. Jana Harichová
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  5. Edita Karelová
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  6. Jana Godočíková
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  7. Bystrík Polek
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  8. Peter Ferianc
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  9. Domenico Pangallo
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Correspondence to Domenico Pangallo.

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Puškárová, A., Bučková, M., Chovanová, K. et al. Diversity and PAH growth abilities of bacterial strains isolated from a contaminated soil in Slovakia. Biologia 68, 587–591 (2013). https://doi.org/10.2478/s11756-013-0193-3

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  • DOI: https://doi.org/10.2478/s11756-013-0193-3

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