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Molecular analysis of plasmid pAP4 from Acetobacter pasteurianus

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Abstract

The complete nucleotide sequence of plasmid pAP4 isolated from Acetobacter pasteurianus 2374T has been determined. Plasmid pAP4 was analysed and found to be 3,870 bp in size with a G+C content of 50.1%. Computer assisted analysis of sequence data revealed 2 possible ORFs with typical promoter regions. ORF1 codes for a protein responsible for kanamycin resistance similar with Tn5 transposone, ORF2 encodes a resistance to ampicillin identical with Tn3 transposone. Plasmid has in A. pasteurianus five copies and in E. coli DH1 about 30 copies per chromosome and it segregation stability in both strains is very high. Based on the data on replication region, plasmid does not code for a replication protein and origin region is similar with ColE1-like plasmid.

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Authors and Affiliations

  1. Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Mlynská dolina B-2, SK-84215, Bratislava 4, Slovakia

    Jozef Grones

  2. Food Research Institute, Priemyselná 4, P.O. Box 25, SK-82475, Bratislava 26, Slovakia

    Miroslava Kretová

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  1. Jozef Grones
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  2. Miroslava Kretová
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Correspondence to Jozef Grones.

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Grones, J., Kretová, M. Molecular analysis of plasmid pAP4 from Acetobacter pasteurianus . Biologia 61, 651–655 (2006). https://doi.org/10.2478/s11756-006-0136-3

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  • DOI: https://doi.org/10.2478/s11756-006-0136-3

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