Skip to content
BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access August 3, 2012

Chemical traits and antimicrobial activity of endemic Teucrium arduini L. from Mt Biokovo (Croatia)

  • Dario Kremer EMAIL logo , Ivna Müller , Valerija Dunkić , Dubravka Vitali , Edith Stabentheiner , Andreas Oberländer , Nada Bezić and Ivan Kosalec
From the journal Open Life Sciences

Abstract

Chemical composition of the essential oil (analysed by GC-FID and GC-MS), the content of macroelements and trace elements (analysed by ICP-AES), and antimicrobial activities were investigated in Teucrium arduini L. from Mt Biokovo (Croatia). Additionally, a study on the types and distribution of glandular trichomes which produce essential oils was investigated. The oil was characterized by a high concentration of sesquiterpene hydrocarbons (68.5%) of which β-caryophyllene (32.9%) and germacrene D (16.4%) being the major compounds. Among the macroelements, the content of calcium was the highest (9772 mg/kg), while the content of sodium was the smallest (117.74 mg/kg). Among the micronutrients, the most represented element was iron (72.07 mg/kg). The content of each investigated toxic metal (As, Hg, Pb, Cd and Cr) was below permissible levels. The essential oils showed antimicrobial activity against bacterial species tested, with MIC values ranging from 6.25 mg/mL to 37.50 mg/mL. Fungal species were susceptible with MIC values from 7.81 mg/mL and 25.00 mg/mL.

[1] Wielgorskaya T., Dictionary of generic names of seed plants, Columbia Univ. Press, New York, 1995 Search in Google Scholar

[2] Gharaibeh M.N., Elayan H.H., Salhab A.S., Hypoglycaemic effects of Teucrium polium, J. Ethnopharmacol., 1988, 24, 93–99 http://dx.doi.org/10.1016/0378-8741(88)90139-010.1016/0378-8741(88)90139-0Search in Google Scholar

[3] Gharaibeh M.N., Elayan H.H., Salhab A.S., Anorexic effect of Teucrium polium in rats, InT. J. Crude Drug Res., 1989, 27, 201–210 Search in Google Scholar

[4] Calis I., Bedir E., Wright A. D., Sticher O., Neoclerodane diterpenoids from Teucrium chamaedrys subsp. Syspirense, J. NaT. Prod., 1996, 59, 457–460 http://dx.doi.org/10.1021/np960242610.1021/np9602426Search in Google Scholar

[5] Sundaresan P.R., Slavoff S.A., Grundel E., White K.D., Mazzola E., Koblenz D., et al., Isolation and characterisation of selected germander diterpenoids from authenticated Teucrium chamaedrys and T. canadense by HPLC, HPLC-MS and NMR, Phytochem. Anal., 2006, 17, 243–250 http://dx.doi.org/10.1002/pca.91210.1002/pca.912Search in Google Scholar

[6] Kušan F., Medicinal and other useful plants, Poljoprivredni nakladni zavod, Zagreb, 1956 Search in Google Scholar

[7] Redžić S.S., The ecological aspect of ethnobotany and ethnopharmacology of population in Bosnia and Herzegovina, Coll. Antropol., 2007, 31, 869–890 Search in Google Scholar

[8] Tutin T.G., Wood D., Teucrium L., In: Heywood V.H., Burges N.A., Moore D.M., Valentine D.H., Walters S.M., Webb D.A. (Eds.), Flora Europaea, Vol. 3, Cambridge Univ. Press, Cambridge, 1972 Search in Google Scholar

[9] Kremer D., Stabentheiner E., Jurišić Grubešić R., Oberländer A., Vladimir-Knežević S., Kosalec I., et al., A morphological and chemotaxonomic study of Teucrium arduini L. in Croatia, and Bosnia and Herzegovina, Plant Biosyst., 2011, doi: 10.1080/11263504.2011.557095 10.1080/11263504.2011.557095Search in Google Scholar

[10] Grzybek J., Phytochemical characteristics of the native Teucrium L. species. Part II. The remaining identified compounds, Diss. Pharm. Pharmacol., 21, 1969, 21, 253–260 Search in Google Scholar

[11] Harborne J.B., Tomás-Barberán F.A., Williams C.A., Gil M.I., A chemotaxonomic study of flavonoids from European Teucrium species, Phytochem., 1986, 25, 2811–2816 http://dx.doi.org/10.1016/S0031-9422(00)83747-410.1016/S0031-9422(00)83747-4Search in Google Scholar

[12] Ulubelen A., Topcu G., Kaya U., Steroidal compounds from Teucrium chamaedrys subsp. chamaedrys, Phytochem., 1994, 36, 171–173 http://dx.doi.org/10.1016/S0031-9422(00)97033-X10.1016/S0031-9422(00)97033-XSearch in Google Scholar

[13] Hisham A., Pathare N., Al-Saidi S., The composition and antimicrobial activity of the essential oil of Teucrium stocksianum subsp. stocksianum leaf from Oman, NaT. Prod. Commun., 2006, 1, 195–199 10.1177/1934578X0600100304Search in Google Scholar

[14] Skočibušić M., Bezić N., Dunkić V., Phytochemical composition and antimicrobial activities of the essential oils from Satureja subspicata Vis. growing in Croatia, Food Chem., 2006, 96, 20–28 http://dx.doi.org/10.1016/j.foodchem.2005.01.05110.1016/j.foodchem.2005.01.051Search in Google Scholar

[15] Adams R.P., Identification of essential oil components by gas chromatography/mass spectroscopy, 4th ed., Allured Publishing Corp., Carol Stream, 2007 Search in Google Scholar

[16] Perkin-Elmer, Flow Injection Mercury/Hydride Analyses Recommended Analytical Conditions and General Information, Bodenseewerk Perkin-Elmer GmbH, Überlingen, 1994 Search in Google Scholar

[17] Jarrel Ash Corporation, ’Trace scan, atom scan 16/25 plasma spectrometer user’s guide’, Jarrel Ash Corporation, Franklin, 1995 Search in Google Scholar

[18] National Committee for Laboratory Standards (NCCLSb), Reference method for broth dilution antifungal susceptibility of yeasts, Approved standards M27-A, National Committee for Laboratory Standards, Wayne, USA, 1997 Search in Google Scholar

[19] National Committee for Laboratory Standards (NCCLSa), Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, Approved standard M7-A6, National Committee for Laboratory Standards, Wayne, USA, 2003 Search in Google Scholar

[20] Payne W.W., A glossary of plant hair terminology, Brittonia, 1978, 30, 239–255 http://dx.doi.org/10.2307/280665910.2307/2806659Search in Google Scholar

[21] Punt W, Hoen P.P., Blackmore S., Nilsson S., Le Thomas A., Glossary of pollen and spore terminology, Rev. PalaeoboT. Palyno., 2007, 143, 1–81 http://dx.doi.org/10.1016/j.revpalbo.2006.06.00810.1016/j.revpalbo.2006.06.008Search in Google Scholar

[22] Formisano C., Rigano D., Senatore F., Al-Hillo M.R.Y., Piozzi F., Rosselli S., Analysis of essential oil from Teucrium maghrebinum Greuter et Burdet growing wild in Algeria, NaT. Prod. Commun., 2009, 5, 411–414 10.1177/1934578X0900400319Search in Google Scholar

[23] Vukovic N., Sukdolak S., Solujic S., Mihailovic V., Mladenovic M., Stojanovic J., et al., Chemical composition and antimicrobial activity of Teucrium arduini essential oil and cirsimarin from Montenegro, J. Med. PlanT. Res., 2011, 5, 1244–1250 Search in Google Scholar

[24] Küçük M., Güleç C., Yasar A., Üçüncü O., Yayli N., Coskunçelebi K., et al, Chemical composition and antimicrobial activities of the essential oils Teucrium chamaedrys subsp. chamaedrys, T. orientale var. puberulens and T. chamaedrys subsp. lydium, Pharm. Biol., 2006, 44, 592–599 http://dx.doi.org/10.1080/1388020060089686810.1080/13880200600896868Search in Google Scholar

[25] Morteza-Semnani K., Saeed M., Akbarzade M., Essential oil composition of Teucrium scordium L., Acta Pharm., 2007, 57, 499–504 http://dx.doi.org/10.2478/v10007-007-0040-610.2478/v10007-007-0040-6Search in Google Scholar

[26] Mohan L., Pant C.C., Melkani A.B., Dev V., Terpenoid composition of the essential oils of Teucrium royleanum and T. quadrifarium, NaT. Prod. Commun., 2010, 5, 939–942 10.1177/1934578X1000500628Search in Google Scholar

[27] Abdulkhader H., Nirmal P., Al-Saidi S., The composition and antimicrobial activity of the essential oil of Teucrium stocksianum subsp. stocksianum leaf from Oman, NaT. Prod. Commun., 2006, 1, 195–199 10.1177/1934578X0600100304Search in Google Scholar

[28] De Martino L., Formisano C., Mancini E., De Feo V., Piozzi F., Rigano D., et al., Chemical Composition and Phytotoxic Effects of Essential Oils from Four Teucrium Species, NaT. Prod. Commun., 2010, 4, 411–414 Search in Google Scholar

[29] Dunkić V., Bezić N., Vuko E., Antiphytoviral activity of essential oil from endemic species Teucrium arduini L., NaT. Prod. Commun., 2011, 6, 1385–1388 10.1177/1934578X1100600940Search in Google Scholar

[30] Jurišić R., Vladimir-Knežević S., Kaloďera Z., Grgić J., Determination of macroelements in Teucrium species growing in Croatia, Acta Pharm., 2001, 51, 311–315 Search in Google Scholar

[31] Jurišić R., Kaloďera Z., Grgić J., Grgić Z., Ćavar S., Determination of copper, cobalt and nickel in Teucrium species growing in Croatia, Acta Pharm., 2001, 51, 75–80 Search in Google Scholar

[32] Shallari S., Schwartz C., Hasko A., Morel J.L., Heavy metals in soils and plants of serpentine and industrial sites of Albania, Sci Total Environ, 1998, 209, 133–142 10.1016/S0048-9697(98)80104-6Search in Google Scholar

[33] Šamec D., Gruz J., Strnad M., Kremer D., Kosalec I., Grubešić R.J., et al., Lucic A., Piljac-Žegarac J., Antioxidant and antimicrobial properties of Teucrium arduini L. (Lamiaceae) flower and leaf infusions antioxidant capacity, Food Chem. Toxicol., 2010, 48, 113–119 http://dx.doi.org/10.1016/j.fct.2009.09.02610.1016/j.fct.2009.09.026Search in Google Scholar

[34] Fahn A., Structure and function of secretory cells, In: Hallahand D.L., Gray J., Callow J.A. (Eds.), Advances in botanical research incorporating advances in plant pathology, Plant trichomes, Vol. 31, Academic Press, London, 2000 10.1016/S0065-2296(00)31006-0Search in Google Scholar

[35] Antunes T., Sevinato-Pinto I., Glandular trichomes of Teucrium scorodonia L. Morphology and histochemistry, Flora, 1991, 185, 65–70 10.1016/S0367-2530(17)30445-0Search in Google Scholar

[36] Lakušić B., Lakušić D., Slavkovska V., Stevanović V., Stevanović B., Morpho-anatomical differentiation of the Balkan endemic species Teucrium arduini L. (Lamiaceae), Arch Biol Sci, 2007, 59, 369–381 http://dx.doi.org/10.2298/ABS0704369L10.2298/ABS0704369LSearch in Google Scholar

[37] Bini Maleci L., Servettaz O., Morphology and distribution of trichomes in Italian species of Teucrium secT. Chamaedrys (Labiatae) — a taxonomical evaluation, Pl. SysT. Evol. 1991, 174, 83–91 http://dx.doi.org/10.1007/BF0093769610.1007/BF00937696Search in Google Scholar

[38] Cavaleiro C., Salgueiro L.R., Antunes T., Sevinate-Pinto I., Barroso J.G., Composition of the essential oil and micromorphology of trichomes of Teucrium salviastrum, an endemic species from Portugal, Flavour Frag. J., 2002, 17, 287–291 http://dx.doi.org/10.1002/ffj.106810.1002/ffj.1068Search in Google Scholar

[39] Ricciardelli D’Albore G., Textbook of melissopalynology, Apimondia Publishing House, Bucharest, 1997 Search in Google Scholar

Published Online: 2012-8-3
Published in Print: 2012-10-1

© 2012 Versita Warsaw

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Downloaded on 26.5.2024 from https://www.degruyter.com/document/doi/10.2478/s11535-012-0077-5/html
Scroll to top button