The paper is devoted to new taxonomic and faunistic data on five Lyssomanes species from French Guiana. Three new species are diagnosed, figured and described: L. aya sp. nov. (♀), L. courtiali sp. nov. (♂♀), and L. rudis sp. nov. (♀). A previously unknown female is described for L. tapirapensis Galiano 1996. New faunistic records are provided for L. remotus Peckham & Peckham 1896 and L. tapirapensis.