The spider genus Thymoites belonging to the family Theridiidae is recorded for the first time from Japan. Two Japanese species, Dipoena okumae YOSHIDA, 1988 and D. chikunii YOSHIDA, 1988 are transferred from the original genus to Thymoites. A new species, Thymoites yaginumai, is described from Yonaguni-jima Island, Okinawa Prefecture.