Thromboxane A2 is Involved in Itch-associated Responses in Mice with Atopic Dermatitis-like Skin Lesions

Authors

  • Tsugunobu Andoh
  • Ai Yamamoto
  • Satomi Haza
  • Koh-ichi Yuhki
  • Fumitaka Ushikubi
  • Shu Narumiya
  • Yasushi Kuraishi

DOI:

https://doi.org/10.2340/00015555-2437

Abstract

To investigate the mechanisms underlying itching in atopic dermatitis, we examined whether thromboxane (TX) A2, an arachidonic acid metabolite, is involved in spontaneous scratching, an itch-related response, in NC mice with atopic dermatitis-like skin lesions. The TXA2 receptor (TP) antagonist ONO-3708 inhibited the spontaneous scratching. The mRNA expression of TX synthase (TXSyn) distributed mainly in epidermis and the concentration of TXB2, a metabolite of TXA2, were increased in lesional skin. Scratching caused by the PAR2 agonist SLIGRL-NH2 was suppressed by ONO-3708. SLIGRL-NH2-induced scratching decreased approximately 75% in TP-deficient mice, compared to wild-type mice. In primary cultures of mouse keratinocytes, SLIGRL-NH2 induced the production of TXA2, as evidenced by the increased TXB2, which was inhibited by the TXSyn inhibitor sodium ozagrel and a PAR2-neutralizing antibody. Taken together, these results suggest that epidermal TXA2, which may be produced via PAR2 activation, is involved in itching in atopic dermatitis.

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Published

2016-04-27

How to Cite

Andoh, T., Yamamoto, A., Haza, S., Yuhki, K.- ichi, Ushikubi, F., Narumiya, S., & Kuraishi, Y. (2016). Thromboxane A2 is Involved in Itch-associated Responses in Mice with Atopic Dermatitis-like Skin Lesions. Acta Dermato-Venereologica, 96(7), 899–904. https://doi.org/10.2340/00015555-2437

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Articles