Abstract
The effect of different concentrations of sodium chloride on the metabolic activity of halophilic bacteria derived from the Lake Gardno estuary was studied using respirometric methods. The influence of salt was examined in two ways: the bacteria were preincubated in various concentrations of sodium chloride, and the cells used for respirometric measurements were supplied with substrates dissolved in water amended with various concentrations of the salt. Generally, an increase in the concentration of sodium chloride in the medium used for preincubation resulted in an increase in metabolic activity. Conversely, a decrease was noted when solutions of increasing salt content were employed to dissolve the substrate. The decrease in oxygen uptake noted when increased amounts of salt were added is proposed to be the result of the short period allowed for adaptation of cells to the higher salt concentrations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
Baxter, R. N. andN. E. Gibbons. 1956. Effects of sodium and potassium chloride on certain enzymes ofMicrococcus halodenitrificans andPseudomonas salinaria.Canadian Journal of Microbiology 2:599–606.
Boring, J., D. J. Kushner, andN. E. Gibbons. 1963. Specificity of the salt requirement ofHalobacterium cutirubrum.Canadian Journal of Microbiology 9:143–154.
Buchanan, R. E. andN. E. Gibbons. 1974. Bergey’s Manual of Determinative Bacteriology, 8th Edition. The Williams and Wilkins Company. Baltimore, Maryland. 1,246 p.
Buckmire, R. andR. A. MacLeod. 1965. Nutrition and metabolism of marine bacteria. XIV. On the mechanism of lysis of a marine bacterium.Canadian Journal of Microbiology 11:677–691.
Daubner, I. 1967. Mikrobiologia Vody (Water Microbiology). Slovenska Academy of Sciences. Bratislava, Czechoslovakia. 447 p.
Drapeau, G. R., T. I. Matula, andR. A. MacLeod. 1966. Nutrition and metabolism of marine bacteria. XV. Relation of Na+ activated transport to the Na+ requirement of a marine Pseudomonad for growth.Journal of Bacteriology 92:63–71.
Ferrer, E. B., B. N. Stapert, andW. Sokolski. 1963. A medium for improved recovery of bacteria from water.Canadian Journal of Microbiology 9:420–422.
Forsyth, M. P. andD. J. Kushner. 1970. Nutrition and distribution of salt response in populations of moderately halophilic bacteria.Canadian Journal of Microbiology 16:253–261.
Kushner, D. J. 1968. Halophilic bacteria.Advances in Applied Microbiology 10:73–99.
Kushwaha, S. C., M. Kates, andJ. K. Kramer. 1977. Occurrence of indole in cells of extremely halophilic bacteria.Canadian Journal of Microbiology 23:826–828.
Lanayi, J. K. 1974. Salt-dependent properties of proteins from extremely halophilic bacteria.Bacteriological Reviews 38:272–290.
Larsen, H. 1981. The family Halobacteriaceae, p. 985–994.In The Procyotes, Springer-Verlag. Berlin, Heidelberg. New York.
MacLeod, R. A., P. Thurman, andH. Rogers. 1973. Comparative transport activity of intact cell membrane vesides and mesosomes ofBacillus licheneformis.Journal of Bacteriology 113:329–340.
Mathemeier, P. F. andR. Y. Morita. 1977. Thermolability of dehydrogeneses from a psychorophilic marine bacterium.Journal of the Oceanographical Society of Japan 33:90–96.
Murdyk, Z. andW. Donerski. 1989. Distribution and physiological properties of heterotrophic bacteria in the estuary lake Gardno.Acta UNC Torun. Limnology Papers 17:27–36.
Onishi, H. 1972. Salt response of amylase produced in media of different NaCl or KCl concentrations by a moderately halophilic Micrococcus.Canadian Journal of Microbiology 118:1617–1620.
Rhodes, N. E. andW. J. Payne. 1962. Further observations on effects of cation on enzyme induction in marine bacteria.Journal of Microbiology and Serology 28:302–314.
Robinson, J. andN. E. Gibbons. 1952. The effect of salt on the growth ofMicrococcus halodenitrificans.Canadian Journal of Botany 30:147–154.
Robinson, J., N. E. Gibbons, andE. S. Tatcher. 1952. A mechanism of halophilism inMicrococcus halodenitrificans.Journal of Bacteriology 64:69–77.
Seki, H., K. V. Stephans, andT. R. Parson. 1969. The contribution of allochthonus bacteria and organic materials from a small river into a semi-enclosed sea.Archiv für Hydrobiologie 66:37–47.
Shewan, J. N., C. Hobbs, andW. Hodgkiss. 1960. A determinative scheme for the identification of certain genera of Gram-negative bacteria with special reference to the Pseudomonaceae.Journal of Applied Bacteriology 23:379–390.
Skerman, V. B. 1967. A Guide to the Identification of the Genera of Bacteria, 7th Edition. The Williams and Wilkins Company, Baltimore, Maryland.
Sorhong, T. andJ. Ordd. 1974. Cell-bound lipase and esterase ofBrevibacterium linens.Applied Microbiology 27:607–608.
Spratt, G. D., J. P. Drozdowski, E. L. Martin, andR. A. MacLeod. 1975. Kinetic of Na+ dependent amino acid transport using cells and membrane vesicles of a marine Pseudomonad.Canadian Journal of Microbiology 21:43–50.
Takahashi, J. N. andN. E. Gibbons. 1959. Effect of salt concentration on the morphology and composition ofMicrococcus halodenitrificans.Canadian Journal of Microbiology 5:25–35.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mudryk, Z., Donderski, W. Effect of sodium chloride on the metabolic activity of halophilic bacteria isolated from the Lake Gardno estuary. Estuaries 14, 495–498 (1991). https://doi.org/10.2307/1352273
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.2307/1352273