Dolichotetranychus zoysiae sp. nov. is described and illustrated from the lawngrasses, Zoysia tenuifolia Willd. (type host) and Z. matrella (L.) Merr. in Okinawa Island, Japan. D. zoysiae sp. nov. resembles D. summersi Pritchard and Baker, 1952, D. tenellae Mohanasundaram, 1983, and D. bakeri El-Enany and Soliman, 1987 in lacking the ventral pair of rostral setae. However, the new species differs from summersi in having 2 pairs of genital setae, from tenellae in having 2 pairs of anal setae, and from bakeri in the presence of 2 setae on the distal segment of palpus.