doiSerbia

Radiotherapeutical chromosomal aberrations in laryngeal cancer patients

Stošić-Divjak Svetlana L. (Klinički centar Srbije, Institut za otorinolaringologiju i maksilofacijalnu hirurgiju, Beograd)
Novaković Ivana V. (Klinički centar Srbije, Centar za kliničku farmakologiju, Beograd)
Kastratović Dragana A. (Medicinski fakultet, Institut za biologiju i humanu genetiku, Beograd)
Pavlović Milan (Institut za medicinu rada i radiološku zaštitu 'Dr Dragomir Karajović', Beograd)
Divjak Isidora D. (Klinički centar Ljubljana, Klinika za otorinolaringologiju i maksilofacijalnu hirurgiju, Ljubljana, Slovenija)
Marković Srđan Z. (Medicinski fakultet, Institut za biologiju i humanu genetiku, Beograd)
Jović Rajko (Klinički centar Vojvodine, Klinika za otorinolaringologiju, Novi Sad)

Introduction. The authors present the results of cytogenetic analysis of 21 patients with laryngeal carcinomas diagnosed and treated in the period 1995-2000 at the Institute of Otorhinolaryngology and Maxillofacial Surgery, Clinical Center of Serbia and Clinical Center of Novi Sad. Material and methods. The patients were specially monitored and the material was analyzed at the Institute of Human Genetics of the School of Medicine in Belgrade as well as in the Laboratory for Radiological Protection of the Institute of Occupational and Radiological Health 'Dr Dragomir Karajovic' in Belgrade. Results. The incidence of chromosomal aberrations and incidence of exchange of material between sister chromatids were observed in the preparation of the metaphasic lymphocyte chromosomes of the peripheral blood obtained in the culture. Structural aberrations were found on the chromosomes in the form of breakups, rings, translocations and dicentrics as early as after a single exposure of patients to tumor radiation dose of 2 Gy in the field sized 5x7. Out of the total number of 35 cultivated blood samples obtained from 13 patients, 21 were successfully cultivated and they were proved to contain chromosomal aberrations. Some of the peripheral blood samples failed to show cell growth in vitro due to the lethal cell damages in vivo. Discussion.. We have consluded that the number of structural aberrations cannot be used as a biological measure of the absorbed ionizing radiation dose. The presence of aberrations per se is indicative of the mutagenic effect of the ionizing radiation, which was also confirmed in our series on the original model by cultivation of the peripheral blood lymphocytes in the culture of the cells of the volunteer donors upon in vitro radiation. Using the method of bromdeoxyuridylreductase, the increased incidence of SCE as a mutagenic effect was registered. Conclusion. It has been concluded that the increase of absorbed radiation dose in vitro leads to prolonged duration of cell cycle in the same conditions, which proves cytostatic effect of radiation. Further fundamental studies are required for clinical implementation of the findings.

Keywords: Laryngeal Neoplasms, Radiotherapy + adverse effects, Chromosome Abberration, Cytogenetic Analysis