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Determination of tramadol in pharmaceutical forms and urine samples using a boron-doped diamond electrode

Keskin Ertuğrul (Department of Analytical Chemistry, Faculty of Pharmacy Adıyaman University, Adıyaman, Turkey)
Allahverdiyeva Shabnam (Department of Biochemistry, Faculty of Science Van Yuzuncu Yil University, Van, Turkey)
Şeyho Esma (Department of Analytical Chemistry, Faculty of Pharmacy Van Yuzuncu Yil University, Van, Turkey)
Yardim Yavuz (Department of Analytical Chemistry, Faculty of Pharmacy Van Yuzuncu Yil University, Van, Turkey)

The present work describes the electroanalytical investigation and a novel voltammetric method for the cheap, fast and simple quantification of tramadol (TRH) using a boron-doped diamond electrode. TRH displayed one well-defined, irreversible and adsorption-controlled oxidation peak at about 1.58 V (vs. Ag/AgCl) in Britton–Robinson buffer (BR, 0.1 mol L-1, pH 3.0) using the cyclic voltammetry technique. The voltammetric responses of the oxidation peaks are dependent on pH and their sensitivity was significantly enhanced in the presence of surfactant media (sodium dodecyl sulphate, SDS). Under the optimized experiment conditions, employing the square-wave stripping mode, it was found that there was an excellent correlation between oxidation peak current and the TRH concentration in the range 0.25 to 50.0 μg mL-1 (8.34×10-7–1.67×10-4 mol L-1), with a detection limit of 0.072 μg mL-1 (2.40×10-7 mol L-1) in 0.1 mol L-1 BR buffer (pH 3.0) solution comprising 8×10-4 mol L-1 SDS at 1.52 V (after 30 s accumulation at open-circuit conditions). The developed approach could be used for the quantification of TRH in pharmaceutical formulations and spiked human urine samples with acceptable recoveries.

Keywords: tramadol, boron-doped diamond electrode, pharmaceutical formulation, urine samples, sodium dodecyl sulfate