Archives of Biological Sciences 2014 Volume 66, Issue 4, Pages: 1393-1400
https://doi.org/10.2298/ABS1404393P
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A method for the rapid detection and identification of halo blight pathogen on common bean
Popović Tatjana (Institute for Plant Protection and Environment, Belgrade)
Balaž Jelica (Faculty of Agriculture, Novi Sad)
Stanković Slaviša (Faculty of Biology, Belgrade)
A diagnostic method based on nested-PCR, followed by ELISA and conventional
bacteriology tests, for the rapid and reliable detection of halo blight
pathogen Pseudomonas savastanoi pv. phaseolicola (Psp) collected from
infected bean leaves and seeds is described. Psp formed white, small and flat
colonies on nutrient agar medium, creamy white, flat and circular on
Milk-Tween agar medium and light yellow, convex and shiny on modified sucrose
peptone agar medium. Eighteen Gram-negative, catalase-positive and
oxidase-negative strains were subjected to nested PCR with primers P 5.1/P
3.1 and P 5.2/P 3.2, which directed the amplification of the 450 bp target
DNA fragment in all tested strains. According to the results of DAS- and
PTA-ELISA with respect to reactivity to specific antibodies, all analyzed
strains belonged to Psp bacterium. Pathogenicity was tested on bean pods and
cotyledon leaves, on which greasy spots were formed. Psp did not cause
hypersensitive reaction on the leaves of tobacco and geranium. Strains
produced levan, fluorescent pigment, oxidative metabolism of glucose, did not
reduce nitrate, did not produce indole and H2S, did not hydrolyze starch,
gelatin and esculin; they produced acid from glucose, mannose, sucrose and
glycerol, and did not produce acid from maltose, starch, esculin, dulcite,
sorbitol, inositol and erythritol.
This article has been retracted. Link to the retraction 10.2298/ABS150609074E
Keywords: Pseudomonas savastanoi pv. Phaseolicola, halo blight, bean, identification