Archives of Biological Sciences 2014 Volume 66, Issue 1, Pages: 307-316
https://doi.org/10.2298/ABS1401307A
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Antioxidant activity and total phenolic and flavonoid contents of Salvia amplexicaulis Lam. extracts
Alimpić Ana (Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Belgrade)
Oaldje Mariana (Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Belgrade)
Matevski V. (Institute of Biology, Faculty of Natural Sciences and Mathematics, University “Sts. Cyril and Methodius”, Skopje, Macedonia + Macedonian Academy of Sciences and Arts, Skopje, Macedonia)
Marin P.D. (Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Belgrade)
Duletić-Laušević Sonja (Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Belgrade)
This study was designed to examine the in vitro antioxidant DPPH free
radical-scavenging activity, and the total phenolic and flavonoid content of
ethanol and methanol extracts of Salvia amplexicaulis Lam. in the whole plant
and different parts, leaves, stems and flowers. The largest amounts of
extract yield were obtained from the flowers, 14.14% and 12.00 % (w/w) in the
ethanol of methanol extracts, respectively. The ethanol extract of leaves
(16.07 μg/ml) and methanol extract of the whole plant (21.28 μg/ml) showed
the highest activity against the DPPH radical. The ethanol extract of the
leaves was the richest in phenols (222.40 mg GAE/g) and flavonoids (49.81 mg
QE/g), whereas the methanol extract of the whole plant contained the highest
amount of phenolics (180.89 mg GAE/g) and flavonoids (38.15 mg QE/g). A very
strong linear correlation between antioxidant activity and the phenolic
content of the extracts was established. The obtained results suggest that S.
amplexicaulis could be regarded as an important source of natural
antioxidants.
Keywords: Salvia amplexicaulis, Lamiaceae, DPPH, phenolics, flavonoids, extracts
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