Archives of Biological Sciences 2014 Volume 66, Issue 1, Pages: 143-148
https://doi.org/10.2298/ABS1401143M
Full text ( 565 KB)
The effects of estradiol and human chorionic gonadotropin on ACHT cells in peripubertal female rats: A histological and stereological study
Milošević Verica (Institute for Biological Research “Siniša Stanković”, Belgrade)
Todorović Danijela (Faculty of Medicine, Kragujevac)
Šošić-Jurjević Branka (Institute for Biological Research “Siniša Stanković”, Belgrade)
Medigović Ivana (Institute for Biological Research “Siniša Stanković”, Belgrade)
Pantelić Jasmina (Institute for Biological Research “Siniša Stanković”, Belgrade)
Ušćebrka Gordana (Faculty of Veterinary Medicine, Novi Sad)
Ajdžanović V. (Institute for Biological Research “Siniša Stanković”, Belgrade)
The effects of estradiol (EDP) and human chorionic gonadotropin (HCG) on
histological and stereological features of adrenocorticotropic (ACTH) cells
in peripubertal female rats were examined. The first group of females
received five injections of EDP (0.25 mg/kg b.w.), every second day from the
4th to 14th day after birth, and was killed at the peripubertal stage. The
second group of females was given two injections of pregnyl-gonadotrophinum
chorionicum (HCG; 50 IU/kg body weight) on the 36th and 37th days after
birth, and it was killed 24 h after the last treatment. The controls were
injected with an equivalent volume of the corresponding vehicle. ACTH cells
were immunohistochemically labeled and stereologically evaluated.
Stereological analysis showed that the volume of ACTH cells and their volume
density in peripubertal females treated with EDP, were decreased by 15.6% and
53.8% (p<0.05), respectively, compared to the controls. In HCG-treated
animals, the observed parameters were increased by 39.2% and 15.4% (p<0.05),
respectively, in comparison with the control females. These findings suggest
that the application of EDP or HCG exerted opposite effects on the
stereological features of pituitary ACTH cells.
Keywords: estradiol, HCG, ACTH cells, histology, stereology
Projekat Ministarstva nauke
Republike Srbije, br. ON 173009