doiSerbia

IRAP variability in Prunus domestica (L.) borkh based on Cassandra retrotransposon polymorphism

Senková Slavomíra (Slovak University of Agriculture in Nitra, Department of Genetics and Plant Breeding, Slovak Republic)
Žiarovská Jana (Slovak University of Agriculture in Nitra, Department of Genetics and Plant Breeding, Slovak Republic)
Fernández Eloy (Czech University of Life Sciences in Prague, Faculty of Tropical AgriSciences, Prague, Czech Republic)
Bošeľová Danka (Slovak University of Agriculture in Nitra, Department of Genetics and Plant Breeding, Slovak Republic)
Bežo Milan (Slovak University of Agriculture in Nitra, Department of Genetics and Plant Breeding, Slovak Republic)

Plum species are reported to possess a wide genomic variability and that is why DNA markers are still actual in the characterization of its germlasm. In this study, twenty-three genotypes of European plums were assessed for the amplified length based polymorphism among the retrotransposon Cassandra insertions in their genomes. The obtained insertional polymorphism caused by the activity of Cassandra showed regional and pedigree differences in the analysed accessions of European plums. Two primers were used in analysis. The first resulted in the amplification in 203 amplicons and the 86.6 % polymorphism. Two unique fragments were obtained for the Torysa and Podolínec varieties using this primer. The second primer resulted in the amplification in 267 amplicons and 74.2 % polymorphism. Four unique fragments were obtained for the plum varieties Švestka domácí, Čačanská ranná and Elena. Hierarchical cluster analysis divided the analysed accessions into the four main clusters. To show the length polymorphism diferences of the analysed genotypes from Germany, Slovakia and former Yugoslavia more pecisely, the scattergram for them was constructed.

Keywords: DNA polymorphism, European plum, retrotransposon marker, variability