Srpski arhiv za celokupno lekarstvo 2023 Volume 151, Issue 1-2, Pages: 21-25
https://doi.org/10.2298/SARH220922003O
Full text (
924 KB)
Low-level laser efficiency in reparation of bone defects
Obradović Radmila (University of Niš, Faculty of Medicine, Niš, Serbia), dr.rada@yahoo.com
Vujović Sanja 
(University of Kragujevac, Faculty of Medical Sciences, Kragujevac, Serbia)
Desnica Jana (University of Kragujevac, Faculty of Medical Sciences, Kragujevac, Serbia)
Stevanović Momir (University of Kragujevac, Faculty of Medical Sciences, Kragujevac, Serbia)
Stanišić Dragana (University of Kragujevac, Faculty of Medical Sciences, Kragujevac, Serbia)
Ognjanović Irena (University of Kragujevac, Faculty of Medical Sciences, Kragujevac, Serbia)
Mikić Mirko (University of Montenegro, Faculty of Medicine, Podgorica, Montenegro)
Bijelić Borivoj (University of Belgrade, School of Dental Medicine, Belgrade, Serbia)
Šubarić Ljiljana (University of Priština - Kosovska Mitrovica, Faculty of Medicine, Kosovska Mitrovica, Serbia)
Đorđević Vladan (University of Travnik, Faculty of Pharmacy and Health, Travnik, Federation of Bosnia and Herzegovina, Bosnia and Herzegovina)
Introduction/Objective. Bone resorption is a common problem in dentistry, and bone reparation cannot be easily achieved. Several techniques of bone grafting and the use of low-level laser treatment (LLLT) as a new therapeutic optional recommended for improving bone repair were applied. The aim of the study was to investigate the influence of LLLT in bone repair of artificially made bone defects in the rat mandible using histomorphometry. Methods. The research was carried out on 60 female rats. Bone defects were made in the mandible, and animals were divided into two groups, each containing 30 animals. In the study group, the implantation site was submitted to GaAlAs laser irradiation 670 nm, 5 mW, 4 minutes per day for 5 days. The control group had no postoperative treatment. The animals were sacrificed after two, six, and eight weeks post LLLT, and preparations were analysed by histomorphometry, determining bone area fraction, bone area, integral density, mean density, and density variation. Results. Histomorphometric analysis revealed statistically higher values of area fraction, area, and integral density in the study group after two and six weeks. However, no beneficial laser effect was noticed after eight weeks. Conclusion. Low-level lasers have a stimulating effect on reparatory mechanisms in the early regeneration stage of artificially made bone defects in the rat mandible and can be used as a useful helping method in bone treatment.
Keywords: bone, low-level laser treatment, osteogenesis
Show references
Prado RF, Ankha MVEA, Griti BDA, Santos ELS, Tini IRP, Ramos CJ, et al. CaP coating and low-level laser therapy to stimulate early bone formation and improve fixation of rough treated implants. Implant Dent. 2018;27(6):660-6. [DOI: 10.1097/ID.0000000000000824] [PMID: 30281536]
Keklicki HB, Yagci A, Yay AH, Goktepe O. Effects of 405-, 532-, 650-, and 940-nm wavelenghts of low-level laser therapies on orthodontic tooth movement in rats. Prog Orthod. 2020;21(43):2- 14. [DOI: 10.1186/s40510-020-00343-3] [PMID: 33258041]
Cunha JLS, de Carvalho FMA, Filho RNP, Ribeiro MAG, de Albuquerque-Junior RLC. Effects of different protocols of low-level laser therapy on collagen deposition in wound healing. Braz Dent J. 2019;30(4):317-74. [DOI: 10.1590/0103-6440201902400] [PMID: 31340220]
Fini MB, Olyaee P, Homayouni A. The effect of low-level laser therapy on the acceleration of orthodontic tooth movement. J Lasers Med Sci. 2020;11(2):204-11. [DOI: 10.34172/jlms.2020.34] [PMID: 32273964]
Al-Shamiri HM, Al-Maweri SA, AlAhmary AW, Aljunayh MS, Aldosari AO, Alqahtani NM, et al. Efficacy of laser therapy for alveolar osteitis: a systematic review of the available evidence. J Evid-Based Dent Pr. 2022;22(2):101711. [DOI: 10.1016/j.jebdp.2022.101711]
Cirak E, Ozyurt A, Peker T, Omeroğlu S, Gungor MN. Comparative evaluation of various low-level laser therapies on bone healing following tooth extraction: An experimental animal study. J CranioMaxill Surg. 2018;46(7):1147-52. [DOI: 10.1016/j.jcms.2018.05.012] [PMID: 29805068]
International Council for Laboratory Animal Science (ICLAS). Available at: www.iclas.org. [Date accessed: April 29, 2022]
Alinica SB, Saglam E, Celik TZ, Hacisalihoglu P, Dogan MA. Is low level laser therapy or ozone therapy more effective for bone healing? Understanding the mechanisms of HIF-1α, RANKL or OPG. Biotech Histochem. 2020;95(8):597-604. [DOI: 10.1080/10520295.2020.1743360] [PMID: 32292090]
Diker N, Aytac D, Heklvacioglu F, Dagdelen C, Oguz Y. Evaluation of the effects of low-level laser therapy on diabetic bone healing. J Craniofac Surg. 2019;30(7):1994-8. [DOI: 10.1097/ SCS.0000000000005654] [PMID: 31232987]
Jafarpour T, Smith F. Design of multi-wavelenght low-level laser therapy device for assisting bone reconstruction applications. Majlesi J Electr Eng. 2020;14(2):117-25. Available at: https://mjee.isfahan.iau.ir/article_696412_aa66ffbd0b3851ead7b50eb54c18a 0f8.pdf
Ribeiro LNS, de Figueiredo FAT, da Silva Mira PC, Arez MFM, Matsumoto MAN, de Menezes LM, et al. Low-level laser therapy (LLLT) improves alveolar bone healing in rats. Lasers Med Sci. 2022;37(2):961-9. [DOI: 10.1007/s10103-021-03340-y] [PMID: 34002343]
Dhiman S, Khan S. Effect of low-level laser therapy (LLLT) on orthodontic tooth movement - cellular level. Adv Dent & Oral Health. 2018;7(5):1-5. [DOI: 10.19080/ADOH.2018.07.555723]
Liu X, Lyon R, Meier HT, Thometz J, Haworth ST. Effect of lowlevel laser therapy on rabbit tibial fracture. Photomed Las Surg. 2007;25(6):487-94. [DOI: 10.1089/pho.2006.2075] [PMID: 18158750]
Lirani-Galvao AP, Jorgetti V, da Silva OL. Comparative study of how low-level laser therapy and low-intensity pulsed ultrasound affect bone repair in rats. Photomed Las Surg. 2006;24(6):735-40. [DOI: 10.1089/pho.2006.24.735] [PMID: 17199474]
Nagasawa A, Negishi A, Kato K. Clinical aplications of LLLT in dental and oral surgery in the Urawa Clinic. Laser therapy. An Int J of Low Level Laser Therapy. 1991;3(3):119-21. [DOI: 10.5978/ islsm.91-OR-17]
Taha SK, Abd El Fattah S, Said E, Abdel-Hamid MA, Nemat AH, Shenawy HE. Effect of laser bio-stimulation on mandibular distraction osteogenesis: an experimental study. J Oral Maxillofac Surg. 2018;76(11):2411-21. [DOI: 10.1016/j.joms.2018.04.030] [PMID: 29856939]
Zheng J, Yang K. Clinical research: ow level laser therapy in accelerating orthodontic tooth movement. BMC Oral Health. 2021;21(1):324. [DOI: 10.1186/s12903-021-01684-z] [PMID: 34182967]
Pretel H, Lizarelli RFZ, Ramalho LTO. Effect of low-level laser therapy on bone repair: histological study in rats. Las Surg Med. 2007;39(10):788-96. [DOI: 10.1002/lsm.20585] [PMID: 18081142]
Farzan A, Khaleghi K, Pirayesh Z. Effect of low-level laser therapy on bone formation in rapid palatal expansion: A systematic review. J Lasers Med Sci. 2022;13:e13. [DOI: 10.34172/jlms.2022.13] [PMID: 35996494]
Hanna R, Dalvi S, Amaroli A, De Angelis N, Benedicenti S. Effects of photobiomodulation on bone defects geafted with bone substitutes: A systematic review of in vivo animal studies. Journal Biophotonics. 2021;14(1):e202000267. [DOI: 10.1002/jbio.202000267] [PMID: 32857463]
Khalil NM, Noureldin MG. Comparison of single versus multiple low-level laser applications on bone formation in extraction socket healing in rabbits (histologic and histomorphometric study). J Oral Maxillofac Surg. 2019;77(9):1760-8. [DOI: 10.1016/j.joms.2019.03.037] [PMID: 31042458]