Pesticidi i fitomedicina 2014 Volume 29, Issue 4, Pages: 237-247
https://doi.org/10.2298/PIF1404237T
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Botrytis cinerea in raspberry in Serbia I: Morphological and molecular characterization
Tanović Brankica (Institute of Pesticides and Environmental Protection, Belgrade)
Hrustić Jovana (Institute of Pesticides and Environmental Protection, Belgrade)
Mihajlović Milica (Institute of Pesticides and Environmental Protection, Belgrade)
Grahovac Mila (Faculty of Agriculture, Novi Sad)
Delibašić Goran (Faculty of Agriculture, Belgrade)
Morphological and molecular characterisation of 130 isolates of Botrytis
cinerea, derived from raspberry fruit originating from six commercial fields
in a raspberry growing region of Serbia (locations: Požega, Prilike, Arilje,
Ivanjica, Šabac and Valjevo) was performed. The results showed that all
isolates formed white, uniform, aerial mycelia with entire margin on PDA
medium. First morphological differences among the isolates appeared after six
days of incubation. Three-week old isolates were grouped into eight distinct
morphological types - four mycelial and four sclerotial. Mostly, they were of
sclerotial type (81.5%) and the most frequently found was an S3 type, which
formed large irregularly placed sclerotia. This type was dominant in five of
six investigated locations and represented 45-65% of the isolates. The least
frequent was the mycelial type M3 (0.7% of the isolates) characterized by
mycelial masses. The presence of Boty and/or Flipper transposons was detected
in isolates originating from all investigated locations. It was discovered
that the B. cinerea population in raspberry in Serbia, besides the
well-described genetically isolated sympatric species transposa (43.1%) and
vacuma (10.8%), contains also another two, boty (44.6%) and flipper (1.5%)
species with only one transposon (either Boty or Flipper) in the genome. In
addition, it was revealed that all isolates from raspberry collected in
Serbia, transposa, vacuma, boty or flipper, are sensitive or weakly resistant
to fenhexamid and therefore belong to the B. cinerea genetical Group II.
Keywords: Botrytis cinerea, Raspberries, Serbia
Projekat Ministarstva nauke Republike Srbije, br. III 46008: Development of
integrated management of harmful organisms in plant production in order to
overcome resistance and improve food quality and safety