Genetika 2018 Volume 50, Issue 1, Pages: 131-141
https://doi.org/10.2298/GENSR1801131S
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Genetic diversity of wheat wild relatives using SSR markers
Salehi Marzeih (Isfahan University of Technology, College of Agriculture, Department of Agronomy and Plant Breeding, Isfahan, Iran)
Arzani Ahmad (Isfahan University of Technology, College of Agriculture, Department of Agronomy and Plant Breeding, Isfahan, Iran)
Talebi Majid (Isfahan University of Technology, College of Agriculture, Department of Agricultural Biotechnology, Isfahan, Iran)
Rokhzadi Asad (Islamic Azad University-Sanandaj Branch, Agronomy Department, Sanandaj, Iran)
Wild relatives of wheat are potential sources of valuable genetic materials
for wheat improvement. Knowledge of the genetic diversity of wild relative
species of wheat is crucial for their conservation and utilization. The
objective of the current study was to investigate the genetic diversity of
inter and intra species of Triticum monococcum ssp. aegilopoides (AA),
Aegilops tauschii (DD) and Aegilops cylindrica (CCDD) originating from
northern and western Iran. Thirty microsatellite (SSR) markers belonging to
A, B, C and D genomes were used for analysis and 20 found to be polymorphic
within and between species. The SSR markers generated a total number of 180
alleles with an average of 9 alleles per locus in 21 genotypes. The genetic
diversity for all loci ranged from 0.74-0.90 with an average of 0.83. The
highest genetic diversity was estimated for Xgwm186 and Xgwm205 which the
latter could amplify in the A, D and CD genomes of T. monococcum, Ae.
tauschii and Ae. cylindrica, respectively. In addition, the number of bands
generated by Xgwm205 along with other four markers in Ae. cylindrica (CD)
was two-fold than that of Ae. tauschii (D). Polymorphic information content
ranged from 0.7-0.89 with an average of 0.82. The dendrogram obtained from
the neighbor-joining method divided the genotypes of the three species into
three distinctive groups. It can be concluded that SSR markers can be useful
not only in differentiating wild species of wheat possessing A, D and C
genomes, but also in assessing the genetic variation of genotypes within
these species.
Keywords: Aegilops spp., Triticum monococcum, SSR markers, wild species