Genetika 2016 Volume 48, Issue 1, Pages: 249-269
https://doi.org/10.2298/GENSR1601249D
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Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines
Dong Liwei (Capital Normal University, College of Life Science, Beijing, China)
Li Ning (Capital Normal University, College of Life Science, Beijing, China)
Lu Xiaobing (Capital Normal University, College of Life Science, Beijing, China)
Prodanovic Slaven (Faculty of Agriculture, Belgrade)
Xu Yanhao (Hubei Collaborative Innovation Center for Grain Industry, Jingzhou, China)
Zhang Wenying (Hubei Collaborative Innovation Center for Grain Industry, Jingzhou, China)
Yan Yueming (Capital Normal University, College of Life Science, Beijing, China + Hubei Collaborative Innovation Center for Grain Industry, Jingzhou, China)
Three wheat glutenin near isogenic lines (NILs) CB037A, CB037B and CB037C
were used to investigate their quality properties and the transcriptional
expression profiles of PDI gene family during grain development. Our purpose
is to understand the relationships between the dynamic expression of
different PDI genes and glutenin allelic compositions related to gluten
quality. The results showed that glutenin allelic variations had no
significant effects on main agronomic traits and yield performance, but
resulted in clear gluten quality changes. CB037B with 5+10 subunits had
higher glutenin macropolymer (GMP) content and better breadmaking quality
than CB037A with 2+12 while the lack of Glu-B3h encoding one abundant
B-subunit in CB037C significantly reduced GMP content, dough strength and
breadmaking quality. The dynamic expression patterns of eight protein
disulfide isomerase (PDI) genes during grain development detected by
quantitative real-time polymerase chain reaction (qRT-PCR) showed the close
correlations between higher expression levels of PDI3-1, PDI5-1 and PDI8-1
and the presence of 5+10 subunits. Meanwhile, Glu-B3h silence resulted in
significant decrease of expression levels of five PDI genes (PDI3-1, PDI5-1,
PDI6-1, PDI7-2 and PDI8-1), suggesting the vital roles of certain PDI genes
in glutenin and GMP synthesis and gluten quality formation.
Keywords: wheat, NILs, glutenins, PDIs, qRT-PCR