Archives of Biological Sciences 2014 Volume 66, Issue 2, Pages: 651-666
https://doi.org/10.2298/ABS1402651R
Full text ( 598 KB)
Cited by
Influence of sampling, storage, processing and optimal experimental conditions on adenylate energy charge in penaeid shrimp
Robles-Romo Arlett (Centro de Investigaciones Biológicas del Noroeste, Instituto Politécnico Nacional, Col. Playa Palo de Santa Rita, La Paz, B.C.S., México)
Arjona Olivia (Centro de Investigaciones Biológicas del Noroeste, Instituto Politécnico Nacional, Col. Playa Palo de Santa Rita, La Paz, B.C.S., México)
Racotta Ilie S. (Centro de Investigaciones Biológicas del Noroeste, Instituto Politécnico Nacional, Col. Playa Palo de Santa Rita, La Paz, B.C.S., México)
Adenylate energy charge (AEC) has been used as a practical index of the
physiological status and health in several disciplines, such as ecotoxicology
and aquaculture. This study standardizes several procedures for AEC
determination in penaeid shrimp that are very sensitive to sampling. We
concluded that shrimp can be frozen in liquid nitrogen and then stored at
-76°C for up to two years for further analysis, or freshly dissected and
immediately homogenized in acid. Other cooling procedures, such as immersion
in cold water or placing shrimp on ice for 15 min resulted in 50% and 73%
decreases in ATP levels, and 9-fold and 10-fold increases in IMP levels,
respectively. Optimal values of AEC (0.9) were obtained in shrimp recently
transferred from ponds to indoor conditions, but decreased to 0.77 after one
month in indoor tanks when stocked at high densities; the AEC re-established
to 0.85 when the shrimps were transferred to optimal conditions (lower
density and dark tanks). While the levels of arginine phosphate followed the
same pattern, its levels did not fully re-establish. Comparison of different
devices for sample homogenization indicated that a cryogenic ball mill mixer
is the more suitable procedure.
Keywords: Adenylic energy charge, ATP, IMP, Litopenaeus (=Penaeus) vannamei, methods, stress