Archives of Biological Sciences 2012 Volume 64, Issue 1, Pages: 135-144
https://doi.org/10.2298/ABS1201135S
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Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)
Savić Jelena (Institute for Biological Research “Siniša Stanković”, Belgrade)
Dragićević Ivana (Faculty of Biology, Belgrade)
Pantelić D. (Faculty of Biology, Belgrade)
Oljača Jasmina (Faculty of Agriculture, Belgrade)
Momčilović Ivana (Institute for Biological Research “Siniša Stanković”, Belgrade)
We have examined the correlation between heat tolerance and small heat shock
protein (sHSP) expression under heat stress conditions in potato (Solanum
tuberosum L.). The relative heat tolerance of nine potato cultivars grown
under greenhouse conditions was determined using the electrolyte leakage
assay (ELA), a standard quantitative assay for heat tolerance. Three
cultivars differing in heat tolerance were selected and designated as
heat-tolerant (‘Laura’), moderately sensitive (‘Liseta’) and heat-sensitive
(‘Agria’) genotypes. The expression of cytosolic HSP18 and chloroplast HSP21
was analyzed at the protein level in the leaves of selected cultivars, both
ex vitro- and in vitro-grown, after heat stress or control treatment.
Immunoblot analysis revealed heat-induced HSP18 and HSP21 expression in all
examined genotypes. A similar pattern of examined sHSP expression was
observed ex vitro and in vitro: heat-tolerant ‘Laura’ accumulated higher
levels of both HSP18 and HSP21 compared to heat-sensitive ‘Liseta’ and
‘Agria’. Our results indicate that ELA combined with immunoblot analysis of
sHSP accumulation under HS conditions, might be considered as a reliable
procedure in screening potato genotypes for heat tolerance. To our knowledge,
this is the first study where sHSP expression between ex vitro- and in
vitro-grown potato plants was compared.
Keywords: Heat stress, heat tolerance, potato, small heat shock proteins, sHSP electrolyte leakage assay