Seed oils and nuts are frequently considered as waste products. During fruit processing of the sweet cherry fruits into processed products the seeds arise as a waste material in the food processing industries. The seeds mainly composed of lipophilic compounds. Therefore, the present work aimed to analyze the physico-chemical composition, nutritive properties and volatile profile of sweet cherry kernel oil extracted by Soxhlet extraction method through ethanol as asolvent which may be useful for therapeutic purpose. It was observed that, the kernels oil contains 57.75±2.70 mg/ml of proteins and 45.46 ±2.69 mg/ml of ascorbic acid and 57.19±0.02mg/ml of tocopherol content. The sun protection factor of oil was found 23.50 against UV-B rays. The key fatty acids present in sweet cherry seed oil (SCSO) was oleic acid (21%), palmitic acid (6.2%), linoleic acid (5.6%) and steric acid (4.3%) respectively. The volatile organic compounds were identified by Gas Chromatography and Mass Spectroscopy (GC-MS) and the highest contents of volatile compounds belong to the category of carboxylic acid, alkanes, alkenes and alcohols. In that Squalene (26.71%), Oleic acid (6.64 %), and Linoleic acid (4.32%) were most abundantly present. The present study showed that oil of cherry seeds usually thrown as waste has high content of vitamin c, tocopherol and various secondary metabolites. Also, the oil comprises presence of vital fatty acid so that it can be use in food industry as well as in therapeutic application.