The present study indicate that there was significant increase in the serum level of eosinophilic cationic protein in both male and female patients with S. scabiei parasite compared to control group, also in total patients with S. scabiei compared to the total control group, this increase may be due to interaction between the human immune response and S. scabiei antigens or influences of products from scabies mites that caused activated eosinophils, neutrophils, basophils and others immune cells which produced in high numbers in allergic inflammation and helminthes infections to produce cytokines, the granulocytes of eosinophil appear in large numbers at inflammatory sites and in response to infections caused by parasites, These granular cells have positively charged proteins, the eosinophil cationic protein is one of four essential proteins that enter the surrounding tissue and stimulate the secretion of the amino acid granules, although ECP varies widely from one patient to another, some studies show that the measurement of this protein by serum monitors many inflammatory diseases, ECP is one of the important  natural defense against invading parasites , allergic inflammatory disease and gastrointestinal diseases (Wardlaw 1994;  Sajad et al., 2017; Majeed and Aljanaby , 2019). The present study similar to study of Czech et al.) 1992) who revealed that serum concentrations of ECP have been related with atopic dermatitis activity and correlated with erythema, papules, pruritus and skin score. The results of study corresponding to study of  Charlotte et al.) 2009) who recorded that concentration of serum ECP was elevated in patients with allergic disease as compared with the healthy controls groups due to elevated blood eosinophil counts, basophils, neutrophils and others immune cells.  The results of study showed a significant increase in the serum level of IgE in male and female patients infected with S. scabiei parasite compared to control group, also in total patients with S. scabiei compared to the total control group, this increase may be due to allergenic protein which secretion from S. scabiei  in host skin lead to type1 hypersensitivity reaction in patients infested  with scabies which is responsible for expelling the parasite and products from the borrows by the intense itching and scratching, which in turn leads to sudden reduction in parasite density at time when itching started (Al-Rawi, 2000; Adam et al., 2019). The sensitization of the host to the mites and its products may be has an important role in pathogenesis of scabies (Van Nest, 1986). The current study agreed with study of (Elmaraghy and Elmeghawry, 2011; Al-Hadraawy and Hessen, 2017) were showed a significant increase in the serum level of IgE in male and female patients infected with S. scabiei parasite compared to control group.  But not agreed with study of Ibrahim et al. (2012)  who revealed that concentration of IgE was lower than its cut off concentration in 50% of patients with scabies, whereas the rest patients with scabies had elevated concentration of IgE. Also agreed with study of Jayaraj et al. (2011) who revealed that levels of IgE immunoglobulin was elevated in patients with scabies in  compared to control group. The current study agreed with study of Maizels et al.  (2005) that showed a significant increase in the serum level of total IgE in patients infected with helminthes parasite infections and allergic diseases in compared to control group.  The results of study showed a significant increase in the serum level of MCP-1 in male and female patients infected with Sarcoptes scabiei parasite compared to control group, also in total patients with S. scabiei compared to the total control group, this increase may be due to interaction between the human immune response and S. scabiei antigens (Sajad et al., 2017; Hayder and Aljanaby, 2019), or may be due to  essential role of  S. scabiei  in demonstrate chemotaxis of neutrophils, eosinophils, macrophages, and Th2- lymphocytes into the skin have shown increased expression levels of keratinocyte-derived chemokines including IL-8, MCP-1, Eotaxin, and Eotaxin-2 (Shiraki et al., 2006; Aljanaby 2013). The results of study agreed with study of Cheng et al. (2010) who reported that the  concentration of MCP-1 elevated in patients with scabies as compared to the total control group due to action of  macrophage migration inhibitory factor which  can induce secretion of TNFa Tumor Necrosis Factor-ב (TNF-ב), INFc Interferon gamma (IFNד), IL-1b, IL-12, IL-6, IL-8 (CXCLD ), MCP-1 and others from mammalian cells and expression of the cell adhesion molecules ICAM-1 and VCAM-1.

The results of study showed a significant increase in the serum level of IL-17 in male and female patients infected with Sarcoptes scabiei parasite compared to control group, also in total patients with S. scabiei compared to the total control group, this increase may be due to hypersensitivity reactions in patients infested with scabies caused by the secretion of different materials by S. scabiei in the host skin (McCarthy et al., 2004; Hengge et al., 2006; Liu et al., 2016; Aljanaby and Medhat 2017). During 4 weeks from infestation of scabies the immunity developed and appears of first symptom (Walton et al., 2008). The human body is facing numerous pathogens every day, but only few of them causing diseases that because we have natural defense system that called innate immune system. It is the first line of host defense (Judith et al., 2013; Mohy et al., 2018 ).Also may by digging the adult female mite in the host skins and stimulated the immune response of host through the life cycle of Sarcoptes scabiei (Currie et al., 2010; Al-Hadraawy and Hessen, 2017). The current results may be due to stimulating many cells of the innate immune system; in particular, they recruit to and activate neutrophils, eosinophils and other immune cells at sites of inflammation, and stimulate endothelial and epithelial cells to synthesize the different  inflammatory cytokines particularly the interleukin-17 which  is associated with hyper-IgE syndrome, also associated with the skin inflammation and producing CD4+ (T-helper)  (Weaver et al., 2007; Kai et al., 2013; Hahn et al., 2016; Aljanaby et al., 2018). The results of current study agreed with study of Luo (2016) who recorded that higher concentration of interleukin-17 and interleukin -23 in crusted scabies are the first to indicate a contribution of Th17 associated cytokines to a dysregulated immune response in crusted scabies pathology (Arlian and Morgan, 2006; Gonzalez-Lombana et al,. 2013; Martin et al., 2014).