초록

Data from our laboratory indicate that application of the mu agonist DAMGO into the basolateral complex of amygdala (BLA) suppresses tail flick reflexes in anesthetized rats. This DAMGO-induced antinociception can be blocked by pretreatment of the BLA with the nonselective opioid antagonist naltrexone, the mu opioid antagonist β-FNA, or the G protein inhibitor pertussis toxin, suggesting that DAMGO's interaction with G protein-coupled mu receptors in the BLA leads to production of antinociception. The present study employing the gene control strategy was conducted to further investigate the direct action of DAMGO on the mu receptors in the BLA. Intra-BLA application of antisense oligodeoxynucleotides (ODN) against mu receptors blocked antinociception following intra-BLA injection of DAMGO. The amount of [3H]-DAMGO binding to mu receptors in the amygdala was also reduced in the antisense ODN-pretreated rats. These data confirm the idea that antinociception induced by DAMGO in the BLA results from a direct interaction of DAMGO with mu receptors in the amygdala.

키워드

Mu receptor; Amygdala; Tail flick; Gene control; Antisense oligodeoxynucleotides (ODN); [3H]-DAMGO binding

참고문헌(50)open

  1. [단행본] Adams, J. U. / 1996 / Functional effects of antisense oligodeoxynucleotides to opioid receptors in rats In: Antisense strategies for the study of receptor mechanisms / Antisense strategies for the study of receptor mechanisms,ed.by RB Raffa and F Porreca,pp.37-52,R.G.Landes Company.? : 37 ~ 52

  2. [학술지] Atweh, S. F. / 1977 / Autoradiographic localization of opiate receptors in rat brain III / The telencephalon.Brain Research 134 : 393 ~ 405

  3. [학술지] Basbaum, A. I. / 1978 / Endogenous pain control mechanisms: Review and hypothesis / Annals of Neurology 4 : 451 ~ 462

  4. [학술지] Basbaum, A. I. / 1984 / Endogenous pain control systems: Brainstem spinal pathways and endorphin circuitry / Annual Review of Neuroscience 7 : 309 ~ 338

  5. [학술지] Bellgowan, P. S. / 1998 / The role of mu and kappa opioid receptors within the periaqueductal gray in the expression of conditional hypoalgesia / Brain Research 279 : 83 ~ 89

  6. [학술지] Bennet, G. J. / 1979 / Inhibition of spinal cord interneurons by narcotic microinjection and focal electrical stimulation in the periaqueductal gray matter / Brain Research 172 : 243 ~ 257

  7. [학술지] Bunzow, R. / 1995 / Characterization and distribution of a cloned rat mu opioid receptor / Journal of Neurochemistry 64 : 14 ~ 24

  8. [학술지] Carstens, E. / 1995 / Midbrain suppression of limb withdrawal and tail flick reflexes in the rat: Correlates with descending inhibition of sacral spinal neurons / Journal of Neurophysiology 73 (6) : 2179 ~ 2194

  9. [학술지] Chen, Y. / 1993 / Molecular cloning and functional expression of a mu-opioid receptor from the rat brain / Molecular Pharmacology 44 : 8 ~ 12

  10. [단행본] De Olmos, J. / 1985 / Amygdala In: The rat nervous system: I. Forebrain and midbrain / Academic Press

  11. [학술지] Emmerson, P. J. / 1994 / Binding affinity and selectivity of opioids at mu, delta and kappa receptors in monkey brain membrane / Journal of Pharmacology & Experimental Therapeutics 271 : 1630 ~ 1637

  12. [학술지] Foo, H. / 2000 / Expression of antinociception in response to a signal for shock is blocked after selective downregulation of mu-opioid receptors in the rostral ventromedial medulla / Molecular Brain Research 76 (2) : 282 ~ 288

  13. [학술지] Helmstetter, F. J. / 1995 / Microinfusion of mu but not delta or kappa agonists into the basolateral amygdala results in inhibition of the tail flick reflex in pentobarbital-anesthetizedrats / Journal of Pharmacology & Experimental Therapeutics 275 : 381 ~ 388

  14. [학술지] Helmstetter, F. J. / 1993 / Inhibition of the tail flick reflex following microinjections of morphine into the amygdala / NeuroReport 4 : 471 ~ 474

  15. [학술지] Helmstetter, F. J. / 1998 / Antinociception following?opioid stimulation of the basolateral amygdala is expressed through the periaqueductal gray and rostral ventromedial medulla / Brain Research 779 : 104 ~ 118

  16. [학술지] Heyman, J. S. / 1988 / Dissociation of opioid antinociception and central gastrointestinal propulsion in the mouse: Studies with naloxonazine / Journal of Pharmacology & Experimental Therapeutics 245 : 238

  17. [학술지] Hopkins, D. A. / 1978 / Amygdaloid projections to the mesencephalon, pons and medulla oblongata in the cat / Experimental Brain Research 32 : 529 ~ 547

  18. [학술지] Kalivas, P. W. / 1982 / Antinociception after microinjection of neurotensin into the central amygdaloid nucleus of the rat / Brain Research 243 : 279 ~ 286

  19. [학술지] Liu-Chen, L.-Y. / 1987 / Covalent labeling of m opioid binding site by [3H]- β-funaltrexamine / Molecular Pharmacology 32 : 321 ~ 329

  20. [학술지] Liu-Chen, L.-Y. / 1995 / Effect of intracerebroventricular β-funaltrexamine on m opioid receptors in the rat brain: consideration of binding condition / Journal of Pharmacology & Experimental Therapeutics 273 : 1047 ~ 1056

  21. [학술지] Mansour, A. / 1994 / Mu-opioid receptor mRNA expression in the rat CNS: comparison to mreceptor binding / Brain Research 643 : 245 ~ 265

  22. [학술지] Mansour, A. / 1987 / Autoradiographic differentiation of mu, delta and kappa opioid receptors in the rat forebrain and midbrain / Journal of Neuroscience 7 : 2445 ~ 2464

  23. [학술지] Mayer, D. J. / 1974 / Pain reduction by focal electrical stimulation of the brain: an anatomical and behavioral analysis / Brain Research 68 : 73 ~ 79

  24. [학술지] McGaraughty, S. / 2004 / Lesions of the periaqueductal gray disrupt input to the rostral ventromedial medulla following microinjections of morphine into the medial or basolateral nuclei of the amygdala / Brain Research : 223 ~ 227

  25. [학술지] Morgan, M. M. / 1991 / Periaqeductal gray stimulation produces a spinally mediated, opioid antinociception for the inflamed hindpaw of the rat / Brain Research 545 : 17 ~ 23

  26. [학술지] Nandigama, P. / 2003 / Affective analgesia following the administration of morphine into the amygdala of rats / Brain Research 959 : 343 ~ 354

  27. [학술지] Nishino,K.,Su,Y.F.,Wong,C.-S.,Watkins,W.D.,& Chang,K.-J / 1990 / Dissociation of mu opioid tolerance from receptor down-regulation in rat spinalcord / Journal of Pharmacology & Experimental Therapeutics 253 : 67 ~ 72

  28. [학술지] Oliveira, M. A. / 2001 / Role of PAG in the antinociception evoked from the medial or central amygdala in rats / Brain Research Bulletin 54 : 55 ~ 63

  29. [학술지] Pan, Z. Z. / 1997 / Cellular mechanism for anti-analgesic action of agonists of the k-opioid receptor / Nature 389 : 382 ~ 385

  30. [학술지] Pan, Y. X. / 2000 / Isolation and expression of a novel alternatively spliced mu opioid receptor isoform, MOR-1F / FEBS Letters 466 : 337 ~ 340

  31. [학술지] Pasternak, D. A. / 2004 / Identification of three alternatively spliced variants of the rat mu opioid receptor gene: dissociation of affinity and efficacy / Journal of Neurochemistry 91 : 881 ~ 890

  32. [학술지] Pasternak, G. W. / 1995 / Mapping of opioid receptors using antisense oligodeoxynucleotides: correlating their molecular biology and pharmacology / Trends in Pharmacological Sciences 16 : 344 ~ 350

  33. [학술지] Paul, D. / 1989 / Different mu receptor subtypes mediate spinal and supraspinal analgesia in mice / European Journal of Pharmacology 168 : 307 ~ 314

  34. [단행본] Paxinos, G. / 1998 / The rat brain in streotaxic coordinates / Academic Press

  35. [학술지] Rizvi, T. A. / 1991 / Connections between the central nucleus of the amygdala and the midbrain periaqueductal gray: Topography and reciprocity / Journal of Comparative Neurology : 121 ~ 131

  36. [학술지] Rossi, G. C. / 1997 / Antisense mapping of MOR-1 in rats: Distinguishing between morphine and morphine-6β-glucuronide antinociception / Journal of Pharmacology & Experimental Therapeutics : 109 ~ 114

  37. [학술지] Rossi, G. C. / 1995 / Antisense mapping the MOR-1 opioid receptor: evidence for alternative splicing and a novel morphine-6b-glucoronide receptor / FEBS Letters 369 : 192 ~ 196

  38. [학술지] Sancez-Blazquez, P. / 1999 / Antisense oligodeoxynucleotides targeting distinct exons of the cloned mu-opioid receptor distinguish between endomorphin-1 and morphine supraspinal antinociception in mice / Antisense Nucleic Acid & Drug Development 9 : 253 ~ 260

  39. [단행본] Schlingensiepen, R. / 1997 / Technical aspects of antisense oligonucleotides: In Antisensefrom technology to therapy: lab manual and text / Blackwell Science : 4 ~ 123

  40. [학술지] Shin,M.-S. / 2005 / Vasoactive intestinal peptide in the amygdala inhibits tail flick reflexes in rats / Brain Research 1040 : 197 ~ 201

  41. [학술지] Shin, M.-S. / 2000 / Pretreatment of the central, but not the basolateral, amygdala with muscimol blocks induction of mu-related antinociception following application of DAMGO / Society for Neuroscience Abstracts 26 : 244 ~ 312

  42. [학술지] Shin, M.-S. / 2005 / Antinociception following application of DAMGO to the basolateral amygdala results from a direct interaction of DAMGO with mu opioid receptors in the amygdala / Brain Research 1064 : 5665

  43. [학술지] Swajkoski, A. R. / 1981 / Blockade by naltrexone of analgesia produced by stimulation of the dorsal raphe nucleus / Pharmacology Biochemistry & Behavior 15 : 419 ~ 423

  44. [학술지] Tam, S. W. / 1986 / Reversible and irreversible binding of beta- funaltrexamine to mu, delta and kappa opioid receptors in guinea pig brain / Journal of Pharmacology & Experimental Therapeutics 239 (2) : 351 ~ 357

  45. [학술지] Terashvili, M. / 2005 / Differential mechanisms of antianalgesia induced by edomorphin-1 and endormorphin-2 in the ventral periaqueductal gray of the rat / Journal of Pharmacology & Experimental Therapeutics 273 (312) : 1257 ~ 1265

  46. [학술지] Tershner, S. A. / 2000 / Antinociception produced by mu opioid receptor activation in the amygdala is partially dependent on activation of mu opioid and neurotensin receptors in the ventral periaqueductal gray / Brain Research 865 : 17 ~ 26

  47. [학술지] Thompson, R. C. / 1993 / Cloning and pharmacological characterization of a rat mu opioid receptor / Neuron 11 : 903 ~ 913

  48. [학술지] Wahlestedt,C. / 1994 / Antisense oligodeoxynucleotide strategies in neuropharmacology / Trends in Pharmacological sciences 15 : 42 ~ 46

  49. [학술지] Wong, C.-S. / 1992 / Continuous intrathecal opioid treatment abolishes the regulatory effects of magnesium and guanine nucleotides on mu opioid receptor binding in rat spinal membranes / Journal of Pharmacology & Experimental Therapeutics 262 : 317 ~ 326

  50. [학술지] Young, R. F. / 1987 / Pain relief by electrical stimulation of the periaqueductal and periventricular gray matter / Journal of Neurosurgery : 364 ~ 371