IN VITRO SCREENING OF ANTIOXIDANT AND ANTIAGING POTENTIAL OF CUCUMIS SATIVUS FRUIT EXTRACT

CHANCHAL GARG; RAVINDER SINGH; MUNISH GARG

doi:10.22159/ajpcr.2020.v13i5.37258

Authors

CHANCHAL GARG Department of Pharmaceutical Sciences, Maharshi Dayanand University Rohtak, Haryana, India.
RAVINDER SINGH Department of Pharmaceutical Sciences, Maharshi Dayanand University Rohtak, Haryana, India.
MUNISH GARG Department of Pharmaceutical Sciences, Maharshi Dayanand University Rohtak, Haryana, India.

DOI:

https://doi.org/10.22159/ajpcr.2020.v13i5.37258

Keywords:

Anti-aging, Anti-wrinkle, Cucumis sativus, Antioxidant, Hyaluronidase, Elastase, Matrix metalloproteinase-1, Tyrosinase

Abstract

Objective: The present study was designed to screen the anti-aging and anti-wrinkle potential of Cucumis sativus fruit through in vitro estimation of antioxidant, anti-hyaluronidase, anti-elastase, anti-collagenase/anti-matrix metalloproteinase (MMP)-1, and anti-tyrosinase activity.

Methods: Raw juice of cucumber was taken, filtered and fractionated with ethyl acetate and n-butanol. The obtained extracts were then evaluated for their antioxidant potential through 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay taking ascorbic acid as positive control and other enzymatic activities in reference to hyaluronidase inhibition, MMP-1/collagenase inhibition, and elastase inhibition taking catechin as reference standard whereas for tyrosinase inhibition the standard used was quercetin.

Results: All the evaluations were performed in triplicates and results were noted down. It was observed that aqueous extract of C. sativus fruits showed a maximum DPPH radical scavenging activity (p<0.0001), half-maximal inhibitory concentration (IC50) at a concentration of 122.67 μg/ml. The ethyl acetate fraction of C. sativus fruits exhibited maximum hyaluronidase (p<0.0001), MMP-1/collagenase (p<0.04), and tyrosinase (p<0.04) inhibitory activity, IC50 at a concentration of 59.54, 45.79, and 24.46 μg/ml, respectively. The elastase (p<0.0001) inhibitory activity by n-butanol fraction of C. sativus fruits extract was maximum, IC50 at a concentration of 52.76 μg/ml.

Conclusion: A potent anti-aging and anti-wrinkle properties were well demonstrated by C. sativus, as depicted from the results obtained.

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