MORPHOFUNCTIONAL CHANGES OF RAT PANCREAS UNDER CONDITIONS ОF NITRIC OXYDE EXCESS

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Background
Nitric oxide (NO) is a fat-soluble gas, a high-reactive and unstable compound, which is formed from L-arginine under the action of NO-synthase.NO easily inpours cell membranes, exists only for a few seconds and being subjected to oxidation transforms to nitrites and nitrates [1].NO acts in all directions as a universal regulator of physiological functions and transmission of nerve impulses, a potent peripheral vasodilator and regulator of motor control and secretion.NO is a potent mediator of inflammation release in response to bacteria, viruses, proinflammatory cytokines [2].
Unlike classic primary messengers, which also regulates smooth muscle function, NO could induce those processes without interaction with receptors and plasma membrane by intracellular activation of cytosolic enzymesoluble guanylate cyclase [3,4].
Increased concentration of guanosine monophosphate activates GMP-dependent protein kinase and Ca 2+ ATPase, which is involved in dephosphorylation of myosin.This leads to the removal of Ca 2+ from the muscle cells and thus to vasodilation [5].
However, other physiological effects of NO, independent of the guanylate cyclase activation, is known, including posttranslational modification of proteins, lipids and other biomolecules.Possible targets of NO is soluble adenosine diphosphate (ADP)-ribosylating enzyme and transcription factors -through those NO may directly affect gene transcription and translation of the mRNA [6].
Despite the fact that many authors describes the protective role of NO, it is also capable of inducing cell damage.The effect of NO on cellular processes is highly relevant and pending problem [7].
Sodium nitroprusside, which is well known as simple NO-donor, can not be synthesized in cells and is an exogenous source of NO, which is traditionally used for experimental studies.
Purpose: to determine the morphofunctional characteristics of the rat pancreas under conditions of NO excess caused by the administration of sodium nitroprusside as a simple NO-donor.
The study was conducted following the standards of the European Convention of Bioethics (1997), European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes, general ethical principles of animal experiments, approved in the law of Ukraine (№ 1759 -VI of 15.12.2009) «On protection of animals from cruel treatment».
To carry out biochemical determinations and histologic study, after excision, total pancreases were excised, trimmed of fat and lymph nodes.Some small pancreatic samples from each rat were immediately fixed in 10% formalin, paraffin-embedded sections were cut at 3-5 microns and mounted on glass slides.Sections were deparaffinized and stained with hematoxylin-eosin or Mallory's trichrome.
Biochemical process of fibrosis was evaluated on the content in the serum of free and protein-bonded hydroxyproline and hexosamines.NO production was determined by the total content of nitrite/nitrate in serum using Gris test.To estimate the exocrine function activity of pancreatic enzymes were measured in serumα-amylase using set of Filisit-diagnosis and trypsin -using Erlanger test with modifications of Shaternikov.
State of the endocrine pancreatic function was evaluated by determining the serum levels of glucose set by «Phyllis-diagnosis» (glucose oxidase method).

Results and Discussion
In 1 and 2 days after the experiment was started, in 100 % of rats dilation of blood vessels and ducts, congestion and stagnation of secretion were observed (Fig. 1).Severity of these symptoms ranged from mild to moderate.

Figure 1 -Rat pancreas after 24 hours (A) and 48 hours (B). Dilation of blood vessels and ducts, congestion and stagnation of secretion. Mallory's trich rome, ×100 Figure 2 -A) rat pancreas in 6 days after beginning of the experiment. Vasodilation and stasis of blood cells, inflammatory infiltration. Mallory's trichrome, ×200; B) rat pancreas in 12 days after beginning of the experiment. Severe dilatation of the vessels with stasis of blood cells, adipose degeneration, ×400; C) rat pancreas in 30 days after beginning of the experiment. Bands of fibrous tissue around the main ducts. Mallory's trichrome, ×100
A B www.gastro.org.ua

Åêñïåðèìåíòàëüí³ äîñë³äaeåííÿ / Experimental Studies
In 6 days of sodium nitroprusside intraperitoneal injections as NO-donor signs of acute pancreatitis were shown, the structural basis of which was inflammationstromal infiltration by lymphocytes and leukocytes, dilation of blood vessels and intralobular ducts, stasis of blood cells.In 66.6 % of all cases big groups of hypersecretory acinar cells were found in parenchyma, in 75.0 % of those among aforementioned cells isolated small foci of necrosis was found.
12 days later the phenomenon of vessels plethora and stasis of blood cells became more pronounced.In 100 % of all cases stroma was diffusely infiltrated by inflammatory cells, excessive accumulation of secretion in the acinar cells was noted with segmental apoptosis of acinar tissue.Focal adipose degeneration was developed in 66.6 %.
Despite the pronounced inflammatory activity, which was observed after 12 days of the experiment, in all animals, sacrificed after 30 days of sodium nitroprusside daily administration, microcirculatory changes were almost entirely offset.Vessel diameter did not differ from the comparison group, infiltration was scarce or not observed.At the same time, in 83.3 % of all cases dilatation of the interlobular ducts and the development of intralobular connective tissue were observed.The tiny bands of fibrous tissue enveloped main ducts, major blood vessels and penetrates into the interlobular space (Fig. 2).
Morphological signs of pancreatitis were accompanied by the changes in biochemical parameters that characterizes metabolism of collagen.Processes of connective tissue anabolism on 30 th day illustrated through content of protein-bonded hydroxyproline in bloodwhich was increased by 1.4 times from (179.28 ± 9.19) μmol/l (control group) to (243.81 ± 15.35) μmol/l (p < 0.01) and catabolism through content of free hydroxyproline -which was increased by 1.5 times (to 16.37 ± 1.39) μmol/l (p < 0.01) and 1.8 times (to 18.01 ± 3.27) μmol/l (p < 0.05) at 12 th and 30 th days, respectively.Compared to controls (9.96 ± 0.71) μmol/l those values indicated increased collagen synthesis and destruction (Fig. 3, 4).It ought to be noted that concentration of the protein-bonded hydroxyproline in the rat blood in 30 days was significantly higher than in one day (p < 0.01), 2 days (p < 0.05) and 6 days (p < 0.05).
Increased concentration of hexosamine (HA) in the blood indicates increased catabolism of carbohydrateprotein components of connective tissue, as HA forms part of the proteoglycans and its components -glycoproteins.Aside from that increasing of HA concentration is a factor that suggest inflammation, while long-term Åêñïåðèìåíòàëüí³ äîñë³äaeåííÿ / Experimental Studies inflammation of the pancreatic tissue may cause its destruction.Leading role in the pancreatic tissue destruction play proteolytic enzymes of polymorphonuclear leukocytes, which induce disintegration of macromolecular complexes containing HA. Probably HA may instigate processes of fibrosis and increasing of its concentration is first indication of changes among other parameters that characterize the functional state of the connective tissue.In this specific experiment, the content of HA in rat blood after 6 days of NG-nitro-L-arginine administration is increased by 1.4 times, to (6.05 ± 0.06) g/l (p < 0.001), after 12 days -by 1.5 times, to (6.42 ± ± 0.17) g/l (p < 0.001), and after 30 days -by 1.8 times, to (7.73 ± 0.06) g/l (p < 0.001) compared with the control group (4.27 ± 0.18) g/l mcM (Fig. 5).Concentration of the in the 30 days was significantly higher than in 1 day (p < 0.001), 2 days (p < 0.01), 6 days (p < 0.001) and 12 days (p < 0.001).
After the first day of experiment slow increase in the activity of α-amylase was noted, with maximum at 6 th day -progress by 2.6 times, from (96.02 ± ± 20.30) mg/s • l (control) to (209.41 ± 38.50) mg/s • l (p < 0.05).However, after 30 days was observed the significant reduction in the activity of this enzyme -by 1.5 times to (65.75 ± 8.03) mg/s • l (p < 0.05) (Fig. 7).Significant difference was observed in α-amylase activity at 6 th day and at 30 th day of sodium nitroprusside administration (p < 0.05).
Trypsin is the best marker for the detection of pancreas pathology, as it is specific to this organ.Activity of this enzyme was varied similarly to α-amylase activity: increasing after 6 days by 2.1 times from (4.19 ± 0.92) μmol/ml • min (control group) to (8.65 ± 1.64) μmol/ml • min (p < 0.05) with a following decrease to the levels of the control group at 30 th day of the experiment (Fig. 8).Trypsin activity in the blood of the experimental animals after 30 days of sodium nitroprusside administration was significantly lower than in group which receives it only 6 days (p < 0.05).
The development of experimental pancreatitis under conditions of NO excess caused by the administration of sodium nitroprusside was accompanied by a gradual violation of glucose metabolism.There was a significant increase (by 1.4 times) of glucose concentration in blood from (3.18 ± 0.42) mmol/l (control group) to (4.34 ± 0.30) mmol/l (p < 0.05) and up to 1.7 times (5.37 ± 0.38) mmol/l (p < 0.01) in 12 and 30 days respectively (Fig. 9).
Thus, the development of pancreatitis was accompanied by the alteration of endocrine function (Fig. 9).
Hence, after 1 and 2 days of sodium nitroprusside administration there was a significant increase of NO metabolites in rat blood (p < 0.001), upward tendency in the activity of pancreatic enzymesα-amylase and trypsin.Morphologically dilation of blood vessels and ducts were observed, alongside with accumulation of secretion in the pancreas.After 6 days the maximum increase in serum enzyme activityα-amylase (p < 0.05) and trypsin (p < 0.05), and of the NO metabolites concentration (p < 0.05) was noted, in addition to gradual increase of the HA concentration (p < 0.001).Morphological signs of acute pancreatitis were shown, the structural basis of which was inflammation -stromal infiltration by lymphocytes and leukocytes, dilation of blood vessels and intralobular ducts, stasis of blood cells, presence of hypersecretory acinar cells and isolated small foci of necrosis.
After 12 days there was a downward tendency in the serum activity of α-amylase and trypsin and significant reduction of the NO metabolites concentration compared to day 6 (p < 0.01).There was the significant increase of the protein-bonded hydroxyproline and free hydroxyproline concentration (p < 0.01) along with HA (p < 0.001).Morphological signs included stromal infiltration and vasodilation.Some acinar cells was in state of focal adipose degeneration or segmental apoptosis.
After 30 days the maximum levels of collagen synthesis was observed -highest concentration of protein-bonded hydroxyproline in the serum (p < 0.01), in parallel with catabolism -highest concentration of free hydroxyproline (p < 0.05) and HA (p < 0.001).Against this background functional failure of the pancreas was developing, which made itself evident in the sharp decrease of the pancreatic enzymes activity -namely α-amylase (p < 0.05) and trypsin (p < 0.05).Reduced concentration of NO metabolites (nitrite/nitrate) was observed after 1 (p < 0.001) and 2 (p < 0.001) days, with gradual increase occurred afterwards -maximum concentration after 6 days (p < 0.05), followed by a gradual decrease -minimum concentration after 30 days (p < 0.001).Following morphological changes after 30 days of sodium nitroprusside administration were observed: compensation of previously affected microcirculation (diameter of blood vessels did not differ from the comparison group); mild dilatation of the interlobular ducts and development of intralobular connective tissue.The tiny bands of fibrous tissue enveloped main ducts, major blood vessels and penetrates into the interlobular space zone, which is typical for chronic pancreatitis.

Conclusions
1. NO excess caused by the intraperitoneal administration of sodium nitroprusside leads to following morphological changes in the pancreas: vasoditation with stasis of blood cells after 1 and 2 days; focal necrosis, destruction of acinar tissue, ductal dilatation and excessive accumulation of secretion after 6 days; adipose degeneration and segmental apoptosis after 12 days and morphologically compensated microcirculatory changes which accompanied the development of fibrous tissue around main ducts, major blood vessels and in the interlobular space after 30 days -changes typical for chronic pancreatitis.
2. Exocrine pancreatic function of rats responded to the excess of NO in ways of increasing pancreatic enzymes levels in blood serumα-amylase and trypsin, which than significantly decrease after 30 days of experiment.The biochemical markers of fibrosis (free and protein-bonded hydroxyproline, hexosamine) also showed increase levels, accompanied with endocrine insufficiency.All this changes is peculiar to development of chronic experimental pancreatitis.
SCHEVCHENKO B.F., BABIY A.M., MAKARCHUK V.A., OSHMYANSKA N.YU., GALINSKY A.A., RUDENKO A.I. State Institution «Institute of Gastroenterology of National Academy of Medical Sciences of Ukraine», Dnipropetrovsk, Ukraine MORPHOFUNCTIONAL CHANGES OF RAT PANCREAS UNDER CONDITIONS ОF NITRIC OXYDE EXCESS Summary.The study was carried out on 40 male Wistar rats after daily intraperitoneal injection of donator of nitric oxide (NO) molecules sodium nitroprusside in a dose of 1.5 mg/kg in 1 day 1 (n = 6); 2 days (n = 6); 6 days (n = 6); 12 days (n = 6); 30 days (n = 6).It is established that an excessive NO intake leads to morphological changes in the pancreas: vasodilation with stasis of blood cells after 1 and 2 days, focal necrosis, destruction of acinar tissue, ductal dilatation with worsening outflow of pancreatic secretion after 6 days; fatty degeneration, segmental apoptosis in 12 days; compensated microcirculatory changes in the body, the formation of fibrous tissue in periductal and perivasal areas with penetration in the interlobular space in 30 days.In the blood serum we detected impaired exocrine pancreatic function: increased activity of pancreatic enzymesα-amylase and trypsin up to 6 days, and in 30 days -they significantly reduced; increasing the levels of biochemical markers of fibrosis (protein-bound hydroxyproline and hexosamine); failure of endocrine function -increase of glucose Гастроентерологія, ISSN 2308-2097 Åêñïåðèìåíòàëüí³ äîñë³äaeåííÿ / Experimental Studies

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Figure 3 -Concentration of protein-bonded hydroxyproline in the rat blood after administration of sodium nitroprusside