بررسی فیتوشیمیایی و فعالیت آنتی‌اکسیدانی فرآورده‌های جانبی اسانس‌گیری ریزوم هیبریدهای زنبق ‏آلمانی (‏Iris germanica L.‎‏)‏

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

2 استادیار، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

3 استادیار، پژوهشکده ملی گل و گیاهان زینتی، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج، محلات، ایران

4 دانشیار، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

چکیده

گونه­های گیاه ایریس (Iris) به عنوان منبع غنی از ایزوفلاونوئیدها شناخته می­شوند. زنبق آلمانی (Iris germanica L.) یکی از مهم­ترین انواع زنبق­های ریش­دار است، که در طب سنتی با نام ایرسا شناخته می­شود. در این پژوهش، میزان کل ترکیبات فنولی و فعالیت آنتی‌اکسیدانی پساب به دست آمده در طول فرآیند اسانس­گیری 34 نمونه ریزوم زنبق آلمانی (6 نمونه والد، 27 نمونه هیبرید و 1 نمونه تجاری) اندازه‌گیری شد. نمونه­های زنبق آلمانی از پژوهشکده ملی گل و گیاهان زینتی واقع در شهر محلات جمع‌آوری و نمونه تجاری از بازار خریداری شدند. بعد از استخراج اسانس‌ از پساب در بالن، نمونه­ برداری شد. میزان فنل کل نمونه‌ها به روش فولین­سیکالتیو، فلاونوئید به روش آلومینیوم کلرید و فعالیت آنتی‌اکسیدانی (DPPH و FRAP) اندازه­گیری شد. نتایج تجزیه واریانس نشان داد که همه صفات اندازه­گیری شده، تحت تاثیر نوع هیبرید بود که این تاثیر در تمامی صفات معنی‌دار بود (01/0P<). نتایج مقایسه میانگین نشان داد که نمونه­های با رنگ گل­های ارغوانی و بنفش بیشترین میزان فنل و فلاونوئید کل را داشته و به دنبال آن بالاترین میزان فعالیت آنتی­اکسیدانی را دارند. به عبارت دیگر، بیشترین میزان فلاونوئید (22/113 میلی­گرم معادل کوئرستین در گرم وزن خشک) و فعالیت آنتی­اکسیدانی (DPPH) (73/817 میلی­گرم معادل TBHQ در گرم وزن خشک) در نمونه P6 (والد ارغوانی) و بیشترین میزان فنل کل (31/261 میلی­گرم معادل اسیدگالیک در گرم وزن خشک) در نمونه P3 (والد بنفش) مشاهده شد. در کل، پساب حاصل از اسانس گیاه زنبق آلمانی، بعنوان منبعی مناسب و ارزان جهت به دست آوردن آنتی‌اکسیدان‌های طبیعی می­باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Phytochemical evaluation and antioxidant activity of essential oil byproducts of ‎German iris hybrids and parents (Iris germanica L.) ‎

نویسندگان [English]

  • Ghader Ghasemi 1
  • Mahdi Ayyari 2
  • Mohammad-Hossein Azimi 3
  • Mohammad-Taghi Ebadi 4
1 Ph.D. Candidate,, Faculty of Agriculture, Tarbiat Modares University (TUM), Tehran, ‎Iran
2 Assistant Professor, Faculty of Agriculture, Tarbiat Modares University (TUM), Tehran, Iran
3 Assistant Professor, National Institute of Ornamental Plants (NIOP), Horticultural Sciences Research Institute, Agricultural ‎Research, Education and Extension Organization (AREEO), Mahallat, I.R. Iran
4 Associate Professor, Faculty of Agriculture, Tarbiat Modares University (TUM), Tehran, Iran
چکیده [English]

Iris species are known to be a rich source of isoflavonoids. Iris germanica L. is one of the most important species of bearded irises which is known as Irsa in traditional medicine. In this study, the amount of phenolic compounds and antioxidant activity of the waste obtained during the essential oil extraction process of 34 samples of German iris rhizomes (6 parent samples, 27 hybrid samples and 1 commercial sample) were evaluated. Iris samples were collected from the Ornamental Plants Research Center (OPRC) in Mahallat city and commercial sample were purchased from the market. After extracting the EO, samplings were carried out using the extract in balloon. The phenol content was measured by Folin-Ciocalteu method, flavonoid by Aluminum-chloride method and antioxidant activity with DPPH and FRAP assay. The results of variance analysis showed that all measured factors were affected by the type of hybrid, which was significant in all factors (P<0.01). The results of mean comparison showed that samples with purple and violet flower colors had the highest amount of phenol and flavonoid, followed by the highest level of antioxidant activity. In other words, the highest amount of flavonoid (113.22 mg QUE/g DW plant) and antioxidant activity (DPPH) (817.73 mg TBHQ/gDW plant) was observed in sample P6 (purple parent) and the highest amount of phenol content (261.31 mg GAE/g DW plant) was observed in sample P3 (violet parent). In general, the water residues from the Iris EO extraction can be used as a suitable and cheap source for obtaining natural antioxidants.

کلیدواژه‌ها [English]

  • By-product
  • Iris
  • Phytochemical
  • Natural antioxidant
  • Waste‎

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صفحه 989-1001

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  • تاریخ دریافت: 25 خرداد 1401
  • تاریخ بازنگری: 20 تیر 1401
  • تاریخ پذیرش: 05 شهریور 1401

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