Caltech Tomography Archive
Published 2020 | Version v1
Dataset Open

lip8-4 (ctrl)

Nicolas, William1
  • 1. ROR icon California Institute of Technology

Description

Exposure 1s

Files

keyimg_wnj2020-09-29-53.jpg
Files (7.2 MB)
Name Size
keyimg_wnj2020-09-29-53.jpg
md5:660e1b352722bf1a882f2d1827862267
1.6 MB Preview Download
keymov_wnj2020-09-29-53.mp4
md5:2fdacd5dfed9925f91fc1ce43e6d72f4
5.6 MB Download

Technical info

Tilt Series Date: 2020-09-29

Data Taken By: William Nicolas

Species / Specimen: Escherichia coli

Strain: PK2, BL21 dHisdHA RQABpCDF+EFGpRSF

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Ų, defocus: -8.0 μm, magnification: 11000x.

Microscope: IECB Talos Artica

Acquisition Software: Serial EM

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Petya Krasteva (head of the SBB lab at the IECB, Bordeaux° and Wiem . . Abidi (graduate student) did the preps and helped with acquisition

Purification / Growth Conditions / Treatment: . . Spheroplasts are generated from the BL21 non transformed cells . cells.. Spheroplasts are processed through a high-pressure . homogenizer and further purification by . ultracentrifugation of the . membrane fractions. . Liposomes are then generated from the membrane . fractions by processing through Potter and sonicator..

Sample Preparation: Fiducials were prepared my method (see lab archive). . Grids: . Quantifoil Cu NH2 R2/2. . Glowdischarged 3mA - 2min. BT3s - BF0. 4uL . pipetted on the grids

External Files

Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-09-29-53

lip8-4_sq1_tg1.mrc, Tilt Series (Pixel Size 0.39 nm), 1.7 GB Download

lip8-4_sq1_tg1_full.rec, Reconstruction (Pixel Size 0.78 nm), 1.8 GB Download

Additional details

Identifiers Funding Dates
Created:
June 7, 2023
Modified:
June 7, 2023