Role of Hexokinase 2 in T Cell Metabolism
PublicT cells and cancer cells have many common features, including their need to proliferate and their metabolic requirements. In particular, glucose metabolism is highly upregulated in both cancer cells and T cells to support proliferation and other cellular functions. The first step of glycolysis is catalyzed by Hexokinase, which exists in 4 main isoforms. Hexokinase 2 (HK2) has been shown to be specifically upregulated in various types of cancers. Moreover, HK2 is required for tumor initiation, growth, and metastasis in different contexts. While this makes HK2 a potential target for cancer therapy, it is important to note that HK2 is also highly upregulated in T cells. It is unclear if inhibition of HK2 would adversely affect T cells by disrupting glucose metabolism. In the context of cancer therapy, T cell inhibition is not desirable, as it leaves patients susceptible to infections and could theoretically interfere with tumor immunity and potential immunotherapies. This thesis explores the necessity of HK2 in T cells in a variety of contexts. HK2 is largely dispensable for T cell activation, proliferation, and differentiation in vitro. In mouse models of inflammation and viral immunity, T cell function is mostly intact in the absence of HK2, with small differences in pathological inflammation. Suppressive T cell function is also spared in vivo when mice are deficient in HK2 in Regulatory T cells. Finally, we identify other potential metabolic difference between leukemic cancer cells and T cells. Together, the data show that HK2 functions mostly as a redundant enzyme in T cells and therefore it may serve as an attractive target for cancer therapy.
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Mehta_northwestern_0163D_14398.pdf | 2020-02-12 | Public |
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