JMIR Publications

ABSTRACT

Background:

Background:

Obesity is known as one of the most major cause of epidemiologic disease worldwide. Therefore, introducing treatment strategies including various medicines to reduce fat or prevent obesity in addition to methods of exercise programs by medical professionals and exercise scientists is on the increase. Recently, researchers have shown special interest in assessing the effect of lipolytic adenosine and vitamin D deficiency. On the other hand, the effect of exercise on decreasing body fat percent has been indicated by many researchers.

Objective:

This research has been designed to examine the effect of injection of adenosine, vitamin D3 and High intensity interval training and isocaloric Moderate intensity training on metabolic parameters in obesity induced by high-fat diet.

Objective:

This research has been designed to examine the effect of injection of adenosine, vitamin D3 and High intensity interval training and isocaloric Moderate intensity training on metabolic parameters in obesity induced by high-fat diet.

Methods:

This is an experimental study. We will select 92 rats and then after getting weight till to 6 weeks will divide in 12 groups. After one week of adaptation to a new environment, 12 groups of Wistar rats will participate in two stages of the experimental intervention including 13 weeks of fattening diet phase followed by 12 weeks of exercise program as exercise training phase and injection of adenosine and vitamin D3. Group 1 and 2 will consume normal diet and other groups of the rats will consume 40% fat diet and have free access to food and water up to the second half of the second stage (end of the sixth week of training). After termination of the interventions, tissue collection and molecular assessments (blood for biochemical, tissues for gene expression analyses and anthropometrical indexes) will be performed.

Results:

The project was founded in April 2017 and completed in December 2017. Data analysis is under way, and the first results are expected to be submitted for publication in Nov 2018.

Conclusions:

We supposed that weight loss induced molecular changes and up regulation will be observed in line with increase in lipolysis and beta oxidation in muscle and fat tissue as a result of performing isocaloric training in drug receiving rats and groups on high fat diet.