Collagen Production by Rat Liver Parenchymal Cells in Primary Culture
1979 Volume 55 Issue 10 Pages 531-536
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1979 Volume 55 Issue 10 Pages 531-536
Rat liver parenchymal and non-parenchymal cells were isolated by the collagenase perfusion method followed by Percoll density gradient centrifugation. The isolated parenchymal cells, when cultured for a few days, formed cords of polygonal cells which are typical for hepatocytes and produced a low (about 0.2% of total protein synthesized) but significant amount of collagen. This is the first demonstration of collagen production by hepatic parenchyma) cells in primary culture.
