The Identification of Hysterothlacium on Sixbar Groupers (Epinephelus sexfasciatus) in The Territorial Waters of East Java Using Sequencing Method

Identifikasi Hysterothylacium sp. pada Ikan Kerapu Belang (Epinephelus sexfasciatus) di Perairan Jawa Timur Menggunakan Metode Sekuensing

Authors

  • Putri Kismawardani Mahasiswa Fakultas Kedokteran Hewan, Universitas Airlangga Surabaya
  • Setiawan Koesdarto Departemen Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga Surabaya
  • Kuncoro Puguh Santoso Departemen Kedokteran Hewan Dasar, Fakultas Kedokteran Hewan, Universitas Airlangga Surabaya
  • Nunuk Dyah Retno Lastuti Departemen Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga Surabaya
  • Emy Koestanti Sabdoningrum Departemen Peternakan, Fakultas Kedokteran Hewan, Universitas Airlangga Surabaya
  • Kusnoto Departemen Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga Surabaya

DOI:

https://doi.org/10.21776/ub.VetBioClinJ.2023.005.01.2

Keywords:

Ephinephelus sexfasciatus, Hysterothylacium amoyense, molecular identification, PCR, squencing

Abstract

This study aimed to identify the parasitic nematode Hysterothylacium sp. using the sequencing method. A sampling of Hysterothylacium sp. was taken from 60 striped grouper fish (Ephinephelus sexfasciatus) and has characteristics such as brown color, booring teeth on the anterior, a simple digestive system including the esophagus, stomach (ventriculus), small intestine and does not have mucron on the posterior. Specific primers for the COX-2 mtDNA gene (210F and 211R) were used for DNA amplification (Polymerase Chain Reaction) and DNA sequencing with a length of 629bp. The positive result of the PCR method (sample S) was sequenced. Then, the results of the sequencing method were compared with the reference DNA sequences KY065298.1, MF120253.1, MF120248.1, and KU886687.1. Furthermore, Homology and kinship tests were carried out using GenBank (NCBI). Results showed a 97% similarity of the sample with Hysterothylacium amoyense cytochrome oxidase subunit II (COX-2) gene MF120253.1.

References

Alifuddin, M., Hadiroseyani, Y., & Ohoiulun, I. (2003). Parasites in Fresh Water Ornamental Fish (Cupang, Guppy and Rainbow Fish). Jurnal Akuakultur Indonesia, 2(2), 93–100.

Awik, P., Hidayati, D., Ressa, P., & Setiawan, E. (2007). Pola Distribusi Anisakis sp pada Usus Halus Ikan Kakap Putih (Lates Calcarifer) yang Tertangkap di TPI Brondong, Lamongan. Prodi Biologi Institut Teknologi Sepuluh Nopember Surabaya, Lab. Zoologi. Alumni Prodi Biologi Institut Teknologi Sepuluh Nopember Surabaya.

Fonseca, M. C. G. da, Knoff, M., Felizardo, N. N., Di Azevedo, M. I. N., Torres, E. J. L., Gomes, D. C., Iñiguez, A. M., & São Clemente, S. C. de. (2016). Integrative taxonomy of Anisakidae and Raphidascarididae (Nematoda) in Paralichthys patagonicus and Xystreurys rasile (Pisces: Teleostei) from Brazil. International Journal of Food Microbiology, 235, 113–124. https://doi.org/10.1016/j.ijfoodmicro.2016.07.026

Hibur, O. S., Detha, A. I. R., Almet, J., & Irmasuryani. (2016). document. Jurnal Kajian Veteriner, 4(2), 40–51.

Kementrian Kelautan dan Perikanan Republik Indonesia. (2016). Konsumsi Ikan Naik dalam 5 Tahun Terakhir. Http://Kkp.Go.Id/2016/03/23/Konsums i-Ikan-Naik-Dalam-5-Tahun-Terakhir/.

Kong, Q., Fan, L., Zhang, J., Akao, N., Dong, K., Lou, D., Ding, J., Tong, Q., Zheng, B., Chen, R., Ohta, N., & Lu, S. (2015). Molecular identification of Anisakis and Hysterothylacium larvae in marine fishes from the East China Sea and the Pacific coast of central Japan. International Journal of Food Microbiology, 199, 1–7. https://doi.org/10.1016/j.ijfoodmicro.2015.01.007

Li, L., Lü, L., Nadler, S. A., Gibson, D. I., Zhang, L. P., Chen, H. X., Zhao, W. T., & Guo, Y. N. (2018). Molecular phylogeny and dating reveal a terrestrial origin in the early carboniferous for ascaridoid nematodes. Systematic Biology, 67(5), 888–900. https://doi.org/10.1093/sysbio/syy018

McPherson, M., & Møller, S. (2006). PCR (2nd ed.). Taylor & Francis.

Pradipta, I. P. G. H., Suratma, N. A., & Oka, I. B. M. (2014). Prevalensi Infeksi Cacing pada Ikan Pisang-pisang (Pterocaesio diagramma) dan Ikan Sulir Kuning (Caesio cuning) yang Dipasarkan di Pasar Ikan Kedonganan, Badung. Buletin Veteriner Udayana, 6(1).

Shamsi, S., & Suthar, J. (2016). Occurrence of Terranova larval types (Nematoda: Anisakidae) in Australian marine fish with comments on their specific identities. PeerJ, 2016(3). https://doi.org/10.7717/peerj.1722

Subekti, S., & Mahasri, G. (2010). Parasit dan Penyakit Ikan (Trematodiasis dan Cestodiasis). Fakultas Perikanan dan Kelautan Universitas Airlangga.

Downloads

Published

2023-07-19

How to Cite

Kismawardani, P., Koesdarto , S., Santoso , K. P., Lastuti , N. D. R., Sabdoningrum , E. K., & Kusnoto. (2023). The Identification of Hysterothlacium on Sixbar Groupers (Epinephelus sexfasciatus) in The Territorial Waters of East Java Using Sequencing Method: Identifikasi Hysterothylacium sp. pada Ikan Kerapu Belang (Epinephelus sexfasciatus) di Perairan Jawa Timur Menggunakan Metode Sekuensing. Veterinary Biomedical and Clinical Journal, 5(1), 11–18. https://doi.org/10.21776/ub.VetBioClinJ.2023.005.01.2

Issue

Vol. 5 No. 1 (2023)

Section

Articles

License

Copyright (c) 2023 Putri Kismawardani, Setiawan Koesdarto , Kuncoro Puguh Santoso , Nunuk Dyah Retno Lastuti , Emy Koestanti Sabdoningrum , Kusnoto

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Most read articles by the same author(s)