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CNS & Neurological Disorders - Drug Targets

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ISSN (Print): 1871-5273
ISSN (Online): 1996-3181

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Research Article

Role of Lipocalin-2 in N1/N2 Neutrophil Polarization After Stroke

Author(s): Zhiliang Guo, Guoli Xu, Jiaping Xu, Yaqian Huang, Chunfeng Liu and Yongjun Cao*

Volume 23, Issue 4, 2024

Published on: 24 May, 2023

Page: [525 - 535] Pages: 11

DOI: 10.2174/1871527322666230417112850

Price: $65

Abstract

Background: Neutrophils and Lipocalin-2 (LCN2) play pivotal roles in cerebral ischemiareperfusion (I/R) injury. However, their contribution is not fully clarified.

Objective: This study aimed to explore the role of LCN2 and its association with neutrophil polarization in I/R injury.

Methods: A mouse model of middle cerebral artery occlusion (MCAO) was used to induce cerebral ischemia. LCN2mAb was administered 1 h and Anti-Ly6G was administered for 3d before MCAO. The role of LCN2 in the polarity transition of neutrophils was explored using an in vitro HL-60 cell model.

Results: LCN2mAb pretreatment had neuroprotective effects in mice. The expression of Ly6G was not significantly different, but the expression of N2 neutrophils was increased. In the in vitro study, LCN2mAb-treated N1-HL-60 cells induced N2-HL-60 polarization.

Conclusion: LCN2 may affect the prognosis of ischemic stroke by mediating neutrophil polarization.

Keywords: Mouse middle cerebral artery occlusion model, lipocalin-2, neutrophil polarization, N2 neutrophils, ischemiareperfusion injury.

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