Abstract
Objective: A simple, economical and rapid RP-HPLC method with PDA detector was developed and validated for the simultaneous determination of simvastatin and caffeine in pure form, in formulation and the method is applied to monitor these drugs in mice plasma. Caffeine containing food and drugs commonly co-administered with statins, there is also a possibility of the caffeine-statins combination as anti-influenza formulation implies a need for a reliable HPLC method for the simultaneous determination of these drugs.
Method: In the present work, the separation was carried out using methanol: water (90:10 v/v) as mobile phase at pH of 3.5 adjusted using phosphoric acid. At room temperature, isocratic separation carried out on C18 XBrigde™ column (5 µm, 25x0.46 cm) at a flow rate of 1 mLmin-1 and drugs were detected at 230 nm by using a PDA detector. The established method was validated with respect to linearity, specificity, precision, accuracy and limits of detection and quantification.
Results: The method was linear at a concentration range of 5-50 µgmL-1 for both drugs with calculated limit of detection of 0.025 and 0.059 µgmL-1 for simvastatin and caffeine, respectively. This method was also successfully applied for the monitoring of these drugs in mice plasma.
Conclusion: The present study suggests that the simultaneous determination of simvastatin and caffeine will be helpful to pharmaceutical manufacturing companies and clinicians checking for drug-drug interactions and could save expenses in case of future combination determination.
Keywords: Simvastatin, caffeine, RP-HPLC, method development, anti-influenza, PDA detector.
Current Analytical Chemistry
Title:Simultaneous Determination of Simvastatin with Caffeine in Bulk Drug, Formulation and their Monitoring in Mice Plasma Through HPLC-PDA Technique
Volume: 13 Issue: 6
Author(s): Majdi M. Bkhaitan*
Affiliation:
- Department of Pharmaceutical Chemistry, Faculty of Pharmacy – Umm Al-Qura University, Makkah- 21955,Saudi Arabia
Keywords: Simvastatin, caffeine, RP-HPLC, method development, anti-influenza, PDA detector.
Abstract: Objective: A simple, economical and rapid RP-HPLC method with PDA detector was developed and validated for the simultaneous determination of simvastatin and caffeine in pure form, in formulation and the method is applied to monitor these drugs in mice plasma. Caffeine containing food and drugs commonly co-administered with statins, there is also a possibility of the caffeine-statins combination as anti-influenza formulation implies a need for a reliable HPLC method for the simultaneous determination of these drugs.
Method: In the present work, the separation was carried out using methanol: water (90:10 v/v) as mobile phase at pH of 3.5 adjusted using phosphoric acid. At room temperature, isocratic separation carried out on C18 XBrigde™ column (5 µm, 25x0.46 cm) at a flow rate of 1 mLmin-1 and drugs were detected at 230 nm by using a PDA detector. The established method was validated with respect to linearity, specificity, precision, accuracy and limits of detection and quantification.
Results: The method was linear at a concentration range of 5-50 µgmL-1 for both drugs with calculated limit of detection of 0.025 and 0.059 µgmL-1 for simvastatin and caffeine, respectively. This method was also successfully applied for the monitoring of these drugs in mice plasma.
Conclusion: The present study suggests that the simultaneous determination of simvastatin and caffeine will be helpful to pharmaceutical manufacturing companies and clinicians checking for drug-drug interactions and could save expenses in case of future combination determination.
Export Options
About this article
Cite this article as:
Bkhaitan M. Majdi *, Simultaneous Determination of Simvastatin with Caffeine in Bulk Drug, Formulation and their Monitoring in Mice Plasma Through HPLC-PDA Technique, Current Analytical Chemistry 2017; 13 (6) . https://dx.doi.org/10.2174/1573411013666170721112505
DOI https://dx.doi.org/10.2174/1573411013666170721112505 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Advances in Microfluidic and Sensing Technologies of Biofluids.
Detection of biological fluids is an important means to assess human health and diagnose diseases. Currently, research on blood is relatively mature and widely applied in the medical field. With the development and advancement of microfluidic technology and novel detection methods, attention has turned to human physiological fluids such as ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Dendritic Cells in Pathogenesis of COPD
Current Pharmaceutical Design Effects of Static Magnetic Fields on Blood Pressure in Animals and Humans
Current Hypertension Reviews Local Renin-Angiotensin II Systems, Angiotensin-Converting Enzyme and its Homologue ACE2: Their Potential Role in the Pathogenesis of Chronic Obstructive Pulmonary Diseases, Pulmonary Hypertension and Acute Respiratory Distress Syndrome
Current Medicinal Chemistry Intracellular Redox State as Target for Anti-Influenza Therapy: Are Antioxidants Always Effective?
Current Topics in Medicinal Chemistry Interactions Between the Sympathetic Nervous System and Angiotensin System in Renovascular Hypertension
Current Hypertension Reviews Is the Cytoskeleton an Intracellular Receptor for Adrenomedullin and PAMP?
Current Protein & Peptide Science Painful Peripheral Neuropathies
Current Neuropharmacology Inhibitors of Nitric Oxide Synthase: What's up and What's Next?
Current Enzyme Inhibition Phospholipase D Inhibition: Beneficial and Harmful Consequences for a Double-Dealer Enzyme
Current Enzyme Inhibition The Effect of Glucose-Insulin-Potassium (GIK) Infusion on Cardiovascular Function and Inflammation: Results from a Phase 1 Pilot Interventional Trial
Applied Clinical Research, Clinical Trials and Regulatory Affairs Neutrophil Gelatinase-Associated Lipocalin (NGAL) as a Biomarker for Acute Kidney Injury
Recent Patents on Biomarkers Age-Related Neurodegeneration Prevention Through mTOR Inhibition: Potential Mechanisms and Remaining Questions
Current Topics in Medicinal Chemistry Nanotechnology for Delivery of Drugs and Biomedical Applications
Current Clinical Pharmacology Recents Patents for Isolating, Delivering and Tracking Adult Stem Cells in Regenerative Medicine
Recent Patents on Drug Delivery & Formulation A Randomized, Double Blind, Controlled, Dose Dependent Clinical Trial to Evaluate the Efficacy of a Proanthocyanidin Standardized Whole Cranberry (Vaccinium macrocarpon) Powder on Infections of the Urinary Tract
Current Bioactive Compounds Tigecycline: A New Treatment Choice Against Acinetobacter baumannii
Recent Patents on Anti-Infective Drug Discovery Pharmacotherapy in Systemic Lupus Erythematosus
Current Rheumatology Reviews The Prevention of Oral Mucositis in Patients with Blood Cancers: Current Concepts and Emerging Landscapes
Cardiovascular & Hematological Agents in Medicinal Chemistry Therapeutic Potential of N-Acetylcysteine for Wound Healing, Acute Bronchiolitis, and Congenital Heart Defects
Current Drug Metabolism Chelating Agents for the Treatment of Systemic Iron Overload
Current Medicinal Chemistry