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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Urea-Induced Denaturation of β-Trypsin: An Evidence for a Molten Globule State

Author(s): Maria Helena Nasser Brumano and Maria Goreti de Almeida Oliveira

Volume 11, Issue 2, 2004

Page: [133 - 140] Pages: 8

DOI: 10.2174/0929866043478257

Price: $65

Abstract

The denaturation of β-trypsin induced by urea was investigated by fluorescence and circular dichroism. A transient denatured state was found at 2 M urea in both intrinsic fluorescence spectrum and bis-(8-anilino-1-naphtalene sulfonate) (bis-ANS) binding. In addition, the absence of tertiary contacts and presence of secondary structure for this state, are consistent with an intermediate equilibrium state having features of molten globule.

Keywords: trypsin, urea denaturation,, molten globule state.


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