A Study on the Anti-inflammatory Activity of Clematis tangutica Using Molecular Networking

Title:A Study on the Anti-inflammatory Activity of Clematis tangutica Using Molecular Networking

Volume: 20

Author(s): Aijing Li, Tao Chen, Shuo Wang, Juyuan Luo, Cheng Shen, Hongmei Li, Yumei Ma, Zhibo Song, Weihang Lu, Denglang Zou*Yulin Li*

Affiliation:

• School of Life Science, Qinghai Normal University, Xining, 810000, P.R. China

• Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, P.R. China

Keywords: Clematis tangutica, LC-MS, Metabolism study, Activity labelled molecular networking, Anti-inflammatory molecules, Structural annotation.

Abstract: Background: Clematis tangutica, an indigenous medicinal herb native to the Qinghai- Tibet Plateau of China, has traditionally been associated with treating various inflammation-related diseases. While its therapeutic potential is recognized, a comprehensive characterization of its metabolite molecules and their anti-inflammatory properties has not been undertaken.

Objective: This study aimed to comprehensively profile the metabolite molecules of Clematis tangutica and identify potential anti-inflammatory active molecules using the Activity Labelled Molecular Networking (ALMN) approach.

Methods: The ALMN approach was employed to visually label activity to the feature-based molecular network, allowing for the profiling of potential anti-inflammatory active molecules in Clematis tangutica. Through correlating activity levels with the respective molecules, a detailed profiling was achieved.

Results: Out of the 8,644 metabolite molecules in Clematis tangutica, ten were identified as the most potent anti-inflammatory molecules. Among these, Spiraeoside was notably annotated along with its structure.

Conclusion: This research successfully identified ten potent anti-inflammatory molecules from the vast metabolite profile of Clematis tangutica, including a detailed annotation of Spiraeoside. This marked a significant step in bridging traditional therapeutic knowledge with modern molecular profiling techniques.

Cite this article as:

Li Aijing, Chen Tao, Wang Shuo, Luo Juyuan, Shen Cheng, Li Hongmei, Ma Yumei, Song Zhibo, Lu Weihang, Zou Denglang*, Li Yulin*, A Study on the Anti-inflammatory Activity of Clematis tangutica Using Molecular Networking, Current Analytical Chemistry 2024; 20 () . https://dx.doi.org/10.2174/0115734110289479240220061031