Abstract
Background: Thermostable alkaline lipases have commercial value and find applications in various industrial and biotechnological sectors such as additives in detergents and food industries, environmental bioremediations and in molecular biology. To use any lipase for esterification or any other application, it is important to purify and characterize the enzyme and study its properties. The aim of this paper to purify and characterize lipase from thermophilic Geobacillus sp.
Methods: Lipase was purified to homogeneity by ammonium salt precipitation and column chromatography i.e. Sepharose column (size 12 × 2 cm) (Sigma Chemicals, USA) and gel filtration chromatography (size 12 × 2 cm) (Sigma Chemicals, USA). The SDS-PAGE was performed on 10% polyacrylamide gel (with 4% stacking gel) using reference molecular weight markers (Genei, Bangalore, India). The purified lipase was further characterized for various parameters.
Results: The results revealed that the enzyme was purified to 5.4-fold after Sephacryl S-300 chromatography. The purified lipase showed a single band on a 10% SDS-PAGE and molecular mass of enzyme was found to be 97 kDa. The purified enzyme of Geobacillus sp. exhibited maximum lipolytic activity at temperature 55°C and pH 9.5. The half life of the lipase of Geobacillus sp. at 55°C was found to be 90 min.
Conclusion: Lipase produced by Geobacillus sp. is most suitable for industrial applications because of its thermostablity, easier production, relatively inexpensive fermentation techniques and alkaline pH range, which enables its use in organic synthesis applications such as synthesis of esters, biodiesel and flavoured compounds.
Keywords: Characterization, Geobacillus sp, lipase, purification, thermophilic.
Graphical Abstract
Call for Papers in Thematic Issues
Age Reversal using transient epigenetc reprogramming with the OSK Yamanaka Factors
Aging is a complex biological process characterized by a gradual deterioration of physiological functions and an increased vulnerability to disease. Recent advancements in the field of regenerative medicine have shown promising avenues for combating aging. Among these, partial epigenetic reprogramming, specifically utilizing the Yamanaka factors, has been shown to reverse ...read more
Bioinformatic Analysis of Regulated Cell Death in Chronic and Acute Inflammatory Diseases
In the intricate realm of inflammatory diseases, delving into the complexities surrounding regulated cell death emerges as an indispensable and forefront area of exploration. Understanding the intricacies of how cells are regulated to undergo death in inflammatory conditions not only represents a pivotal frontier in biomedical research but also holds ...read more
Related Journals
Related Books
Research Methodology and Project Management in Biotechnology
Recent Progress in Pharmaceutical Nanobiotechnology: A Medical Perspective
Recent Trends In Livestock Innovative Technologies
Algal Biotechnology for Fuel Applications
Terpenoids: Recent Advances in Extraction, Biochemistry and Biotechnology
Recent Advances in Biotechnology
28