Abstract
Immobilization of proteins and other biomolecules in saccharide matrices leads to a series of peculiar properties that are relevant from the point of view of both biochemistry and biophysics, and have important implications on related fields such as food industry, pharmaceutics, and medicine. In the last years, the properties of biomolecules embedded into glassy matrices and/or highly concentrated solutions of saccharides have been thoroughly investigated, at the molecular level, through in vivo, in vitro, and in silico studies. These systems show an outstanding ability to protect biostructures against stress conditions; various mechanisms appear to be at the basis of such bioprotection, that in the case of some sugars (in particular trehalose) is peculiarly effective.
Here we review recent results obtained in our and other laboratories on ternary protein- sugar-water systems that have been typically studied in wide ranges of water content and temperature. Data from a large set of complementary experimental techniques provide a consistent description of structural, dynamical and functional properties of these systems, from atomistic to thermodynamic level.
In the emerging picture, the stabilizing effect induced on the encapsulated systems might be attributed to a strong biomolecule-matrix coupling, mediated by extended hydrogen-bond networks, whose specific properties are determined by the saccharide composition and structure, and depend on water content.Keywords: Biopreservation, glasses, myoglobin, protein dynamics, trehalose.
Current Organic Chemistry
Title:Proteins in Saccharides Matrices and the Trehalose Peculiarity: Biochemical and Biophysical Properties
Volume: 19 Issue: 17
Author(s): Lorenzo Cordone, Grazia Cottone, Antonio Cupane, Antonio Emanuele, Sergio Giuffrida and Matteo Levantino
Affiliation:
Keywords: Biopreservation, glasses, myoglobin, protein dynamics, trehalose.
Abstract: Immobilization of proteins and other biomolecules in saccharide matrices leads to a series of peculiar properties that are relevant from the point of view of both biochemistry and biophysics, and have important implications on related fields such as food industry, pharmaceutics, and medicine. In the last years, the properties of biomolecules embedded into glassy matrices and/or highly concentrated solutions of saccharides have been thoroughly investigated, at the molecular level, through in vivo, in vitro, and in silico studies. These systems show an outstanding ability to protect biostructures against stress conditions; various mechanisms appear to be at the basis of such bioprotection, that in the case of some sugars (in particular trehalose) is peculiarly effective.
Here we review recent results obtained in our and other laboratories on ternary protein- sugar-water systems that have been typically studied in wide ranges of water content and temperature. Data from a large set of complementary experimental techniques provide a consistent description of structural, dynamical and functional properties of these systems, from atomistic to thermodynamic level.
In the emerging picture, the stabilizing effect induced on the encapsulated systems might be attributed to a strong biomolecule-matrix coupling, mediated by extended hydrogen-bond networks, whose specific properties are determined by the saccharide composition and structure, and depend on water content.Export Options
About this article
Cite this article as:
Cordone Lorenzo, Cottone Grazia, Cupane Antonio, Emanuele Antonio, Giuffrida Sergio and Levantino Matteo, Proteins in Saccharides Matrices and the Trehalose Peculiarity: Biochemical and Biophysical Properties, Current Organic Chemistry 2015; 19 (17) . https://dx.doi.org/10.2174/1385272819666150429232426
DOI https://dx.doi.org/10.2174/1385272819666150429232426 |
Print ISSN 1385-2728 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5348 |
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