Infective Endocarditis Caused by Panton-Valentine Leukocidin-producing Methicillin-susceptible Staphylococcus aureus Identified by the Broad-range PCR Method

Hiroshi Nakajima; Kaoru Dohi; Masaki Tanabe; Akiko Nakamura; Shinji Kanemitsu; Hideo Wada; Norikazu Yamada; Tsutomu Nobori; Hideto Shinpo; Masaaki Ito

doi:10.2169/internalmedicine.55.5403

CASE REPORTS

Infective Endocarditis Caused by Panton-Valentine Leukocidin-producing Methicillin-susceptible Staphylococcus aureus Identified by the Broad-range PCR Method

Hiroshi Nakajima, Kaoru Dohi, Masaki Tanabe, Akiko Nakamura, Shinji Kanemitsu, Hideo Wada, Norikazu Yamada, Tsutomu Nobori, Hideto Shinpo, Masaaki Ito

Author information

Hiroshi Nakajima
Department of Cardiology and Nephrology, Mie University Graduate School of Medicine, Japan
Kaoru Dohi
Department of Cardiology and Nephrology, Mie University Graduate School of Medicine, Japan
Masaki Tanabe
Department of Cardiology and Nephrology, Mie University Graduate School of Medicine, Japan
Akiko Nakamura
Central Clinical Laboratories, Mie University Hospital, Japan
Shinji Kanemitsu
Department of Thoracic and Cardiovascular Surgery, Mie University Graduate School of Medicine, Japan
Hideo Wada
Department of Molecular and Laboratory Medicine, Mie University Graduate School of Medicine, Japan
Norikazu Yamada
Department of Cardiology and Nephrology, Mie University Graduate School of Medicine, Japan
Tsutomu Nobori
Department of Molecular and Laboratory Medicine, Mie University Graduate School of Medicine, Japan
Hideto Shinpo
Department of Thoracic and Cardiovascular Surgery, Mie University Graduate School of Medicine, Japan
Masaaki Ito
Department of Cardiology and Nephrology, Mie University Graduate School of Medicine, Japan

Keywords: infective endocarditis, polymerase chain reaction, Panton-Valentine leukocidin-producing methicillin-susceptible Staphylococcus aureus, heart failure, valve surgery

JOURNAL OPEN ACCESS

2016 Volume 55 Issue 14 Pages 1871-1875

DOI https://doi.org/10.2169/internalmedicine.55.5403

Details

Abstract

A 76-year-old man was admitted to a community hospital due to a persistent high fever. He became afebrile after the administration of broad-spectrum antibiotics, but developed heart failure due to progressive aortic and mitral valve insufficiency and was transferred to our hospital. Although sequential blood cultures were negative, a broad-range polymerase chain reaction targeting the bacterial 16S-rRNA gene followed by the direct sequencing of whole blood revealed spa(+), mecA(-) and Panton-Valentine leukocidin (PVL)(+). He was finally diagnosed with infective endocarditis (IE) caused by PVL-producing methicillin-susceptible Staphylococcus aureus (MSSA), and underwent cardiac surgery. This is the first reported case of IE due to MSSA producing PVL.

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