Abstract
L-Carnitine is a naturally occurring compound that facilitates the transport of fatty acids into mitochondria for β-oxidation. Exogenous L-carnitine is used clinically for the treatment of carnitine deficiency disorders and a range of other conditions.
In humans, the endogenous carnitine pool, which comprises free L-carnitine and a range of short-, medium- and long-chain esters, is maintained by absorption of L-carnitine from dietary sources, biosynthesis within the body and extensive renal tubular reabsorption from glomerular filtrate. In addition, carrier-mediated transport ensures high tissue-to-plasma concentration ratios in tissues that depend critically on fatty acid oxidation. The absorption of L-carnitine after oral administration occurs partly via carrier-mediated transport and partly by passive diffusion. After oral doses of 1–6g, the absolute bioavailability is 5–18%. In contrast, the bioavailability of dietary L-carnitine may be as high as 75%. Therefore, pharmacological or supplemental doses of L-carnitine are absorbed less efficiently than the relatively smaller amounts present within a normal diet.
L-Carnitine and its short-chain esters do not bind to plasma proteins and, although blood cells contain L-carnitine, the rate of distribution between erythrocytes and plasma is extremely slow in whole blood. After intravenous administration, the initial distribution volume of L-carnitine is typically about 0.2–0.3 L/kg, which corresponds to extracellular fluid volume. There are at least three distinct pharmacokinetic compartments for L-carnitine, with the slowest equilibrating pool comprising skeletal and cardiac muscle.
L-Carnitine is eliminated from the body mainly via urinary excretion. Under baseline conditions, the renal clearance of L-carnitine (1–3 mL/min) is substantially less than glomerular filtration rate (GFR), indicating extensive (98–99%) tubular reabsorption. The threshold concentration for tubular reabsorption (above which the fractional reabsorption begins to decline) is about 40–60 µmol/L, which is similar to the endogenous plasma L-carnitine level. Therefore, the renal clearance of L-carnitine increases after exogenous administration, approaching GFR after high intravenous doses.
Patients with primary carnitine deficiency display alterations in the renal handling of L-carnitine and/or the transport of the compound into muscle tissue. Similarly, many forms of secondary carnitine deficiency, including some drug-induced disorders, arise from impaired renal tubular reabsorption. Patients with end-stage renal disease undergoing dialysis can develop a secondary carnitine deficiency due to the unrestricted loss of L-carnitine through the dialyser, and L-carnitine has been used for treatment of some patients during long-term haemodialysis. Recent studies have started to shed light on the pharmacokinetics of L-carnitine when used in haemodialysis patients.
Similar content being viewed by others
References
Bahl JJ, Bressler R. The pharmacology of carnitine. Annu Rev Pharmacol Toxicol 1987; 27: 257–77
Bieber LL. Carnitine. Annu Rev Biochem 1988; 57: 261–83
Bremer J. The role of carnitine in cell metabolism. In: De Simone C, Famularo G, editors. Carnitine today. Austin: Landes Bioscience, 1997: 1–37
Carter AL, Abney TO, Lapp DF. Biosynthesis and metabolism of carnitine. J Child Neurol 1995; 10 Suppl. 2: 3–7
Peluso G, Barbarisi A, Savica V, et al. Carnitine: an osmolyte that plays a metabolic role. J Cell Biochem 2000; 80: 1–10
Pons R, De Vivo DC. Primary and secondary carnitine deficiency syndromes. J Child Neurol 1995; 10 Suppl. 2: 2S8–24
Famularo G, Matricardi F, Nucera E, et al. Carnitine deficiency: primary and secondary syndromes. In: De Simone C, Famularo G, editors. Carnitine today. Austin: Landes Bioscience, 1997: 119–61
Arsenian MA. Carnitine and its derivatives in cardiovascular disease. Prog Cardiovasc Dis 1997; 40(3): 265–86
Pepine CJ. The therapeutic potential of carnitine in cardiovascular disorders. Clin Ther 1991; 13(1): 1–21
De Simone C, Moretti S, Marcellini S, et al. L-Carnitine a modulator of immunometabolic homeostasis in subjects infected with the human immunodeficiency virus. In: De Simone C, Famularo G, editors. Carnitine today. Austin: Landes Bioscience, 1997: 215–31
Murakami K, Sugimoto T, Woo M, et al. Effect of L-carnitine supplementation on acute valproate intoxication. Epilepsia 1996; 37(7): 687–9
Bohan TP, Helton E, McDonald I, et al. Effect of L-carnitine treatment for valproate-induced hepatotoxicity. Neurology 2001; 56 (2 of 2): 1405–8
Kelly GS. L-Carnitine: therapeutic applications of a conditionally-essential amino acid. Altern Med Rev 1998; 3(5): 345–60
Pearson DJ, Tubbs PK, Chase JF. Carnitine and acetylcarnitines. In: Bergmeyer M, editor. Methods of enzymatic analysis. New York (NY): Academic Press, 1974: 1758–71
Pande SV, Caramancion MN. A simple radioisotopic assay of acetylcarnitine and acetyl-CoA at picomolar levels. Anal Biochem 1981; 112(1): 30–8
Pande SV. Radioisotopic assay of acetylcarnitine and acetyl-Co-A. Methods Enzymol 1986; 123: 259–63
Cooper MB, Forte CA, Jones DA. Citrate interference with the determination of acetylcarnitine: a method for its elimination. Clin Chim Acta 1986; 159(3): 291–9
Cederblad G, Carlin JI, Constantin-Teodosiu D, et al. Radioisotopic assays of Co ASH and carnitine and their acetylated forms in human skeletal muscle. Anal Biochem 1990; 185(2): 274–8
Fritz IB, Schulz SK, Srere PA. Properties of partially purified carnitine acetyltransferase. J Biol Chem 1963; 238: 2509–17
Marquis NB, Fritz IB. Enzymological determination of free carnitine concentrations in rat tissue. J Lipid Res 1964; 5: 184–7
Cederblad G, Lindstedt S. Excretion of L-carnitine in man. Clin Chim Acta 1971; 33: 117–23
Wieland OH, Deufel T, Paetzke-Brunner I. Free and esterified carnitine-colorimetric method. In: Bergmeyer H, editor. Methods of enzymatic analysis. Weinheim: Verlag Chemie, 1985: 481–8
Sandor A, Cseko J, Alkonyi I. Use of anion-exchange resin in F-form in sample processing for determination of carnitine. J Chromatogr 1989; 497: 250–7
Shihabi ZK, Oles KS, McCormic CP, et al. Serum and tissue carnitine assay based on dialysis. Clin Chem 1992; 38 (8 pt 1): 1414–7
Deufel T. Determination of L-carnitine in biological fluids and tissues. J Clin Chem Clin Biochem 1990; 28(5): 307–11
Maehara M, Kinoshita S, Watanabe K. A Simple fluorometric method for the determination of serum free carnitine. Clin Chim Acta 1988; 171(2–3): 311–6
Tegelaers FP, Pickkers MG, Seelen PJ. Effect of deproteinization and reagent buffer on the enzymatic assay of L-carnitine in serum. J Clin Chem Clin Biochem 1989; 27(12): 967–72
Xia LJ, Folkers K. Improved methodology to assay carnitine and levels of free and total carnitine in human plasma. Biochem Biophys Res Commun 1991; 176(3): 1617–23
Cederblad G, Harper P, Lindgren K. Spectrophotometry of carnitine in biological fluids and tissue with a Cobas Bio Centrifugal Analyzer. Clin Chem 1986; 32(2): 342–6
Harper P, Elwin CE, Cederblad G. Pharmacokinetics of bolus intravenous and oral doses of L-carnitine in healthy subjects. Eur J Clin Pharmacol 1988; 35: 69–75
Pearson DJ, Tubbs PK. A sensitive enzymic assay for carnitine. Biochem J 1964; 91(1): 2C–4C
Bellinghieri G, Savica V, Mallamace A, et al. Correlation between increased serum and tissue L-carnitine levels and improved muscle symptoms in hemodialyzed patients. Am J Clin Nutr 1983; 38: 523–31
Cederblad G, Lindstedt S. A method for the determination of carnitine in the picomole range. Clin Chim Acta 1972; 37: 235–43
Bohmer T, Rydning A, Solberg HE. Carnitine levels in human serum in health and disease. Clin Chim Acta 1974; 57: 55–61
McGarry JD, Foster DW. An improved and simplified radioisotopic assay for the determination of free and esterified carnitine. J Lipid Res 1976; 17: 277–83
Parvin R, Pande S. Microdetermination of (−) carnitine and carnitine acetyltransferase activity. Anal Biochem 1977; 79(1–2): 190–201
Brass EP, Hoppel CL. Carnitine metabolism in the fasting rat. J Biol Chem 1978; 253(8): 2688–93
Christiansen RZ, Bremer J. Acetylation of tris(hydroxymethyl)aminomethane (tris) and tris derivatives by carnitine acetyltransferase. FEBS Lett 1978; 86(1): 99–102
Pace JA, Wannemacher RW, Neufeld HA. Improved radiochemical assay for carnitine and its derivatives in plasma and tissue extracts. Clin Chem 1978; 24(1): 32–5
Fishlock RC, Bieber LL, Snoswell AM. Sources of error in determinations of carnitine and acylcarnitine in plasma. Clin Chem 1984; 30(2): 316–8
De Sousa C, English NR, Stacey TE, et al. Measurement of L-carnitine and acylcarnitine in body fluids and tissues in children and in adults. Clin Chim Acta 1990; 187: 317–28
Kerner J, Bieber LL. A radioisotopic-exchange method for quantitation of short-chain (acid-soluble) acylcarnitines. Anal Biochem 1983; 134: 459–66
Schmidt-Sommerfeld E, Penn D, Kerner J, et al. Analysis of acylcarnitines in normal human urine with the radioisotopic exchange-high performance liquid chromatography (HPLC) method. Clin Chim Acta 1989; 181(2): 231–8
Schmidt-Sommerfeld E, Penn D, Rinaldo P, et al. Urinary medium-chain acylcarnitines in medium-chain acyl-CoA dehydrogenase deficiency, medium-chain triglyceride feeding and valproic acid therapy: sensitivity and specificity of the radioisotopic exchange/high performance liquid chromatography method. Pediatr Res 1992; 31(6): 545–51
Schmidt-Sommerfeld E, Penn D, Duran M, et al. Detection of inborn errors of fatty acid oxidation from acylcarnitine analysis of plasma and blood spots with the radioisotopic exchange-high-performance liquid Chromatographic method. J Pediatr 1993; 122 (5 pt 1): 708–14
Schmidt-Sommerfeld E, Zhang L, Bobrowski PJ, et al. Quantitation of short- and medium-chain acylcarnitines in plasma by radioisotopic exchange/high-performance liquid chromatography. Anal Biochem 1995; 231(1): 27–33
Minkler PE, Ingals ST, Hoppel CL. High-performance liquid Chromatographic separation of acylcarnitines following derivatization with 4′-bromophenacyl trifluoromethanesulfonate. Anal Biochem 1990; 185: 29–35
Minkler PE, Hoppel CL. Determination of free carnitine and ‘total’ carnitine in human urine: derivatization with 4′-bromophenacyl trifluoromethanesulfonate and high performance liquid chromatography. Clin Chim Acta 1992; 212(1–2): 55–64
Minkler PE, Hoppel CL. Quantification of carnitine and specific acylcarnitines by high-performance liquid chromatography: application to normal human urine and urine from patients with methylmalonic aciduria, isovaleric acidemia or mediumchain acyl-CoA dehydrogenase deficiency. J Chromatogr 1993; 613(2): 203–21
Minkler PE, Hoppel CL. Quantification of free carnitine individual short- and medium-chain acylcarnitines and total carnitine in plasma by high-performance liquid chromatography. Anal Biochem 1993; 212(2): 510–8
Minkler PE, Brass EP, Hiatt WR, et al. Quantification of carnitine, acetylcarnitine and total carnitine in tissues by highperformance liquid chromatography: the effect of exercise on carnitine homeostasis in man. Anal Biochem 1995; 231: 315–22
Van Kempen TA, Odle J. Quantification of carnitine esters by high-performance liquid chromatography. J Chromatogr 1992; 584(2): 157–65
Bhuiyan AK, Jackson S, Turnbull DM, et al. The measurement of carnitine and acyl-carnitine: application to the investigation of patients with suspected inherited disorders of mitochondrial fatty acid oxidation. Clin Chim Acta 1992; 207(3): 185–204
Kamimori H, Hamashima Y, Konishi M. Determination of carnitine and saturated-acyl group carnitines in human urine by high-performance liquid chromatography with fluorescence detection. Anal Biochem 1994; 218(2): 417–27
Kuroda N, Ohyama Y, Nakashima K, et al. HPLC determination of carnitine and acylcarnitines in human plasma by means of fluorescence labeling using 2-(4-hydrazino-carbonylphenyl)-4,5-dipenylimidazole. Chem Pharm Bull (Tokyo) 1996; 44(8): 1525–9
Longo A, Bruno G, Curti S, et al. Determination of L-carnitine acetyl-L-carnitine and propionyl-L-carnitine in human plasma by high-performance liquid chromatography after pre-column derivitization with l-aminoanthracene. J Chromatogr B Biomed Appl 1996; 686(2): 129–39
Schmidt-Sommerfeld E, Penn D. Comments on quantitation of carnitine esters by high performance liquid chromatography. J Chromatogr 1994; 652: 115–6
Van Kempen TA, Odle J. Comments on quantification of carnitine esters by high performance liquid chromatography. Reply to E. Schmidt-Sommerfeld and D. Penn. J Chromatogr 1994; 652: 117–8
Minkler PE, Ingalls ST, Hoppel CL. Comments on quantification of carnitine esters by high performance liquid chromatography. Reply to E. Schmidt-Sommerfeld and D. Penn. J Chromatogr 1994; 652: 119–21
Roe CR, Millington DS, Maltby DA, et al. Diagnostic and therapeutic implications of medium-chain acylcarnitines in the medium-chain acyl-CoA dehydrogenase deficiency. Pediatr Res 1985; 19(5): 459–66
Lowes S, Rose ME. Simple and unambiguous method for identifying urinary acylcarnitines using gas chromatography-mass spectrometry. Analyst 1990; 115(5): 511–6
Lowes S, Rose ME, Mills GA. Identification of urinary acylcarnitines using gas chromatography-mass spectrometry: preliminary clinical applications. J Chromatogr 1992; 577(2): 205–14
Huang ZH, Gage DA, Bieber LL, et al. Analysis of acylcarnitines as their N-demethylated ester derivatives by gas chromatography-chemical ionization mass spectrometry. Anal Biochem 1991; 199(1): 98–105
Millington DS, Roe CR, Maltby DA. Application of high resolution fast atom bombardment and constant B/E ration linked scanning to the identification and analysis of acylcarnitines in metabolic disease. Biomed Mass Spectrom 1984; 11(5): 236–41
Yergey AL, Liberato DJ, Millington DS. Thermospray liquid chromatography/mass spectrometry for the analysis of L-carnitine and its short-chain acyl derivatives. Anal Biochem 1984; 139(2): 278–83
Norwood DL, Kodo N, Millington DS. Application of continuous-flow liquid chromatography/fast-atom bombardment mass spectrometry to the analysis of diagnostic acylcarnitines in human urine. Rapid Commun Mass Spectrom 1988; 2(12): 269–72
Gaskell SJ, Guenat C, Millington DS. Differentation of isomeric acylcarnitines using tandem mass spectrometry. Anal Chem 1986; 58(13): 2801–5
Kodo N, Millington DS, Norwood DL, et al. Quantitative assay of free and total carnitine using tandem mass spectrometry. Clin Chim Acta 1989; 186(3): 383–90
Johnson AW, Mills K, Clayton PT. The use of automated electrospray ionization tandem MS for the diagnosis of inborn errors of metabolism from dried blood spots. Biochem Soc Trans 1996; 24(3): 932–8
Vreken P, Van Lint AE, Bootsma AH, et al. Quantitative plasma acylcarnitine analysis using electrospray tandem mass spectrometry for the diagnosis of organic acidaemias and fatty acid oxidation defects. J Inherit Metab Dis 1999; 22(3): 302–6
Stevens RD, Hillman SL, Worthy S, et al. Assay for free and total carnitine in human plasma using tandem mass spectrometry. Clin Chem 2000; 46(5): 727–9
Chace DH, DiPerna JC, Mitchell BL, et al. Electrospray tandem mass spectrometry for analysis of acylcarnitines in dried postmortem blood specimens collected at autopsy from infants with unexplained cause of death. Clin Chem 2001; 47(7): 1166–82
Rashed MS, Ozand PT, Bucknall MP, et al. Diagnosis of inborn errors of metabolism from blood spots by acylcarnitines and amino acids profiling using automated electrospray tandem mass spectrometry. Pediatr Res 1995; 38(3): 324–31
Wiley V, Carpenter K, Wilcken B. Newborn screening with tandem mass spectrometry: 12 months’ experience in NSW Australia. Acta Paediatr Suppl 1999; 88(432): 48–51
Naylor EW, Chace DH. Automated tandem mass spectrometry for mass newborn screening for disorders in fatty acid, organic acid and amino acid metabolism. J Child Neurol 1999; 14 Suppl. 1: S4–8
Abdenur JE, Chamoles NA, Guinle AE, et al. Diagnosis of isovaleric acidaemia by tandem mass spectrometry: false positive result due to pivaloylcarnitine in a newborn screening programme. J Inherit Metab Dis 1998; 21: 624–30
Johnson DW. Inaccurate measurement of free carnitine by the electrospray tandem mass spectrometry screening methods for blood spots. J Inherit Metab Dis 1999; 22(2): 201–2
Chace DH, DiPerna JC, Adam BW, et al. Errors caused by the use of D,L-octanolycarnitine for blood-spot calibrators. Clin Chem 2001; 47(4): 758–60
Panzetta G, Bonadonna G, Giovene P, et al. Carnitine kinetics during dialysis. Nephron 1985; 41: 230–4
Rizza V, Lorefice R, Rizza N, et al. Pharmacokinetics of L-carnitine in human subjects. In: Ferrari R, Dimauro S, Sherwood G, editors. L-Carnitine and its role in medicine: from function to therapy. New York: Academic Press, 1992: 63–77
Uematsu T, Itaya T, Nishimoto M, et al. Pharmacokinetics and safety of l-carnitine infused I.V. in healthy subjects. Eur J Clin Pharmacol 1988; 34: 213–6
Welling PG, Thomsen JH, Shug AL, et al. Pharmacokinetics of L-carnitine in man following intravenous infusion of dl-carnitine. Int J Clin Pharmacol Biopharm 1979; 17: 56–60
Brass EP, Hoppel CL, Hiatt WR. Effect of intravenous L-carnitine on carnitine homeostasis and fuel metabolism during exercise in humans. Clin Pharmacol Ther 1994; 55: 681–92
Gloggler A, Bulla M, Furst P. Kinetics of intravenously administered carnitine in haemodialysed children. J Pharm Biomed Anal 1990; 3(5): 411–4
Sahajwalla CG, Helton ED, Purich ED, et al. Multiple-dose pharmacokinetics and bioequivalence of L-carnitine 330mg tablet versus 1g chewable tablet versus enterai solution in healthy adult male volunteers. J Pharm Sci 1995; 84(5): 627–33
Sahajwalla CG, Helton ED, Purich ED, et al. Comparison of L-carnitine pharmacokinetics with and without baseline correction following administration of single 20mg/kg intravenous dose. J Pharm Sci 1995; 84(5): 634–9
Segre G, Bianchi E, Corsi M, et al. Plasma and urine pharmacokinetics of free and of short-chain carnitine after administration of carnitine in man. Arzneimittel Forschung 1988; 38 (II) (12): 1830–4
Evans AM, Faul R, Fornasini G, et al. Pharmacokinetics of L-carnitine in patients with end-stage renal disease undergoing long-term hemodialysis. Clin Pharmacol Ther 2000; 68(3): 238–49
Pace S, Longo A, Toon S, et al. Pharmacokinetics of propionyl-L-carnitine in humans: evidence for saturable tubular reabsorption. Br J Clin Pharmacol 2000; 50(5): 441–8
Kerner J, Hoppel C. Fatty acid import into mitochondria. Biochim Biophys Acta 2000; 1486(1): 1–17
Rebouche CJ, Seim H. Carnitine metabolism and its regulation in microorganisms and mammals. Annu Rev Nutr 1998; 18: 39–61
Vaz FM, Wanders RJA. Carnitine biosynthesis in mammals. Biochem J 2002; 361: 417–29
Lombard KA, Olson AL, Nelson SE, et al. Carnitine status of lactoovovegetarians and strict vegetarian adults and children. Am J Clin Nutr 1989; 50(2): 301–6
Rebouche CJ. Carnitine function and requirements during the life cycle. FASEB J 1992 Dec; 6: 3379–86
Rebouche CJ. Metabolic fate of dietary carnitine in humans. In: Lee Carter A, editor. Current concepts in carnitine research. Boca Raton & Ann Arbor: CRC Press, 1992: 37–48
Cederblad G. Effect of diet on plasma carnitine levels and urinary carnitine excretion in humans. Am J Clin Nutr 1987; 45(4): 725–9
Mitchell ME, Snyder EA. Dietary carnitine effects on carnitine concentrations in urine and milk in lactating women. Am J Clin Nutr 1991; 54: 814–20
Stadler DD, Chenard CA, Rebouche CJ. Effect of dietary macronutrient content on carnitine excretion and efficiency of carnitine reabsorption. Am J Clin Nutr 1993; 58: 868–72
Rebouche CJ, Chenard CA. Metabolic fate of dietary carnitine in human adults: identification and quantification of urinary and fecal metabolites. J Nutr 1991; 121(4): 539–46
Rebouche CJ, Lombard KA, Chenard CA. Renal adaptation to dietary carnitine in humans. Am J Clin Nutr 1993; 58: 660–5
Mancinelli A, Longo A, Shanahan K, et al. Disposition of Incarnitine and acetyl-L-carnitine in the isolated perfused rat kidney. J Pharmacol Exp Ther 1995; 274(3): 1122–8
Marzo A, Martelli EA, Mancinelli A, et al. Protein binding of L-carnitine family components. Participants Papers (Special Issue No III). Eur J Drug Metab Pharmacokinet 1991, 364–8
Frolich J, Seccombe DW, Hahn P, et al. Effect of fasting on free and esterified carnitine levels in human serum and urine: correlation with serum levels of free fatty acids and b-hydroxybutyrate. Metabolism 1978; 27(5): 555–61
Engel AG, Rebouche CJ, Wilson DM, et al. Primary systemic carnitine deficiency: II. renal handling of carnitine. Neurology 1981; 31: 819–25
Stanley CA, Berry GT, Bennett MJ, et al. Renal handling of carnitine in secondary carnitine deficiency disorders. Pediatr Res 1993; 34: 89–97
Bernardini I, Rizzo WB, Dalakas M, et al. Plasma and muscle free carnitine deficiency due to renal Fanconi syndrome. J Clin Invest 1985; 75: 1124–30
Ohashi R, Tamai I, Nezu J, et al. Molecular and physiological evidence for multifunctionality of carnitine/organic cation transporter OCTN2. Mol Pharmacol 2001; 59(2): 358–66
Tamai I, Ohashi R, Nezu J, et al. Molecular and functional identification of sodium ion-dependent, high affinity human carnitine transporter OCTN2. J Biol Chem 1998; 273(32): 20378–82
Wagner CA, Lukewille U, Kaltenbach S, et al. Functional and pharmacological characterization of human Na+-carnitine cotransporter hOCTN2. Am J Physiol Renal Physiol 2000; 279: F584–91
Wang Y, Ye J, Ganpathy V, et al. Mutations in the organic cation/carnitine transporter OCTN2 in primary carnitine deficiency. Proc Natl Acad Sci U S A 1999; 96: 2356–60
Wu X, Huang W, Prasad PD, et al. Functional characteristics and tissue distribution pattern of organic cation transporter 2 (OCTN2), an organic cation/carnitine transporter. J Pharmacol Exp Ther 1999; 290: 1482–92
Nezu J, Tamai I, Oku A, et al. Primary systemic carnitine deficiency is caused by mutations in a gene encoding sodium ion-dependent carnitine transporter. Nat Genet 1999; 21(1): 91–4
Tang NL, Ganpathy V, Wu X, et al. Mutations of OCTN2, an organic cation/carnitine transporter, lead to deficient cellular carnitine uptake in primary carnitine deficiency. Hum Mol Genet 1999; 8: 655–60
Angelini C, Vergani L, Martinuzzi A. Clinical and biochemical aspects of carnitine deficiency and insufficiency: transport defects and inborn errors of β-oxidation. Crit Rev Clin Lab Sci 1992; 29(3–4): 217–42
Berteli M, Battistella PA, Vergani L, et al. Carnitine deficiency induced during hemodialysis and hyperlipidemia: effect of replacement therapy. Am J Clin Nutr 1981; 34: 1496–500
Moorthy AV, Rosenblum M, Rajaram R, et al. A comparison of plasma and muscle carnitine levels in patients on peritoneal or hemodialysis for chronic renal failure. Am J Nephrol 1983; 3: 205–8
Brooks DE. Carnitine in the male reproductive tract and its relation to the metabolism of the epididymis and spermatozoa. In: Frenkel R, McGarry J, editors. Carnitine biosynthesis metabolism and function. New York: Academic Press, 1980: 219–35
Brass EP. Pharmacokinetic considerations for the therapeutic use of carnitine in hemodialysis patients. Clin Ther 1995; 17(2): 176–85
Cederblad G, Lindstedt S, Lundholm K. Concentration of carnitine in human muscle tissue. Clin Chim Acta 1974; 53: 311–21
Brass EP. Carnitine transport. In: Ferrari R, Dimauro S, Sherwood G, editors. Carnitine and its role in medicine from function to therapy. New York: Academic Press, 1992: 21–36
Harper P, Wadstrom C, Cederblad G. Carnitine measurements in liver, muscle tissue, and blood in normal subjects. Clin Chem 1993; 39(4): 592–9
Debska-Slizien A, Kawecka A, Wojnarowski K, et al. Correlation between plasma carnitine muscle carnitine and glycogen levels in maintenance hemodialysis patients. Int J Artif Organs 2000; 23(2): 90–6
Savica V, Bellinghieri G, Di Stefano C, et al. Plasma and muscle carnitine levels in haemodialysis patients with morphological-ultrastructural examination of muscle samples. Nephron 1983; 35: 232–6
Rebuzzi AG, Schiavoni G, Lanza GA, et al. Rhythm of carnitine levels in serum and urine of normal subjects. Drugs Exp Clin Res 1989; XV: 43–6
Wanner C, Horl WH. Carnitine abnormalities in patients with renal insufficiency: pathophysiological and therapeutical aspects. Nephron 1988; 50: 89–102
Cooper MB, Forte CA, Jones DA. Carnitine and acetylcarnitine in red blood cells. Biochim Biophys Acta 1988; 959: 100–5
Arduini A, Rossi M, Mancinelli G, et al. Effect of L-carnitine and acetyl-L-carnitine on human erythrocyte membrane stability and deformability. Life Sci 1990; 47: 2395–400
Browne TR. Stable isotopes in clinical pharmacokinetic investigations: advantages and disadvantages. Clin Pharmacokinet 1990; 18(6): 423–33
Rebouche CJ. Quantitative estimation of absorption and degradation of a carnitine supplement by human adults. Metabolism 1991; 40(12): 1305–10
Rebouche CJ, Engel AG. Kinetic compartmental analysis of carnitine metabolism in the human carnitine deficiency syndromes. J Clin Invest 1984 Mar; 73(3): 857–67
Gudjonsson H, Li B, Shug AL, et al. In vivo studies of intestinal carnitine absorption in rats. Gastroenterology 1985; 88(6): 1880–7
Matsuda K, Yuasa H, Watanabe J. Physiological mechanismbased analysis of dose-dependent gastrointestinal absorption of L-carnitine in rats. Biopharm Drug Dispos 1998; 19: 465–72
Matsuda K, Yuasa H, Watanabe J. Fractional absorption of L-carnitine after oral administration in rats: evaluation of absorption site and dose dependency. Biol Pharm Bull 1998; 21(7): 752–5
Gross CJ, Henderson LM, Savaiano DA. Uptake of L-carnitine, D-carnitine and acetyl-L-carnitine by isolate guinea-pig enterocytes. Biochim Biophys Acta 1986; 886: 425–33
Shaw RD, Li B, Hamilton JW, et al. Carnitine transport in rat small intestine. Am J Physiol 1983; 245: G376–81
Hamilton JW, Li B, Shug AL, et al. Carnitine transport in human intestinal biopsy specimens: demonstration of an active transport system. Gastroenterology 1986; 91(1): 10–6
McCloud E, Ma TY, Grant KE, et al. Uptake of L-carnitine by human intestinal epithelial cell line, Caco 2. Gastroenterology 1996; 111(6): 1534–40
Gross CJ, Henderson LM. Absorption of D- and L-carnitine by the intestine and kidney tubule in the rat. Biochim Biophys Acta 1984; 772: 209–19
Gudjonsson H, Li B, Shug AL, et al. Studies of carnitine metabolism in relation to intestinal absorption. Am J Physiol 1985; 248 (3 pt 1): G313–9
Rebouche C, Mack D, Edmonson P. L-Carnitine dissimilation in the gastrointestinal tract of the rat. Biochemistry 1984; 23: 6422–6
Li B, Lloyd ML, Gudjonsson H, et al. The effect of enterai carnitine administration in humans. Am J Clin Nutr 1992; 55: 838–45
Baker H, Frank O, DeAngelis B, et al. Absorption and excretion of L-carnitine during single or multiple dosing in humans. Int J Vitam Nutr Res 1993; 63(1): 22–6
Mancinelli A, Longo A, Nation RL, et al. Disposition of L-carnitine and its short-chain esters, acetyl-L-carnitine and propionyl-L-carnitine in the rat isolated perfused liver. Drug Metab Dispos 2000; 28(12): 1401–4
Rebouche C, Engel A. Kinetic compartmental analysis of carnitine metabolism in the dog. Arch Biochem Biophys 1983; 220: 60–70
Dippenaar N, Clauss RP, Feinendegen LE. Effect of carnitine and essential fatty acid supplementation on the uptake of 11Ccarnitine in muscle of a myopathic carnitine-deficient patient using positron emission scintigraphy. Prostaglandins Leukot Essent Fatty Acids 1998; 58(3): 201–4
Brooks DE, Mclntosh JE. Turnover of carnitine by rat tissues. Biochem J 1975; 148(3): 439–45
Cederblad G, Lindstedt S. Metabolism of labeled carnitine in the rat. Arch Biochem Biophys 1976; 175: 173–80
Khairallah EA, Wolf G. Carnitine decarboxylase: the conversion of carnitine to β-methylcholine. J Biol Chem 1967; 242(1): 32–9
Zhang AQ, Mitchell SC, Smith RL. Discontinuous distribution of n-oxidation of dietary-derived trimethylamine in a British population. Xenobiotica 1996; 26(9): 957–61
Zhang AQ, Mitchell SC, Smith RL. Dietary precursors of trimethylamine in man: a pilot study. Food Chem Toxicol 1999; 37: 515–20
Ayesh R, Mitchell SC, Zhang A, et al. The fish odour syndrome: biochemical familial and clinical aspects. BMJ 1993; 307(6905): 655–7
Humbert JR, Hammond KB, Hathaway WE, et al. Trimethylaminuria: the fish-odour syndrome. Lancet 1970; II(7676): 770–1
Mitchell SC. The fish-odour syndrome. Perspect Biol Med 1996; 39(4): 514–26
Mitchell SC, Zhang AQ, Smith RL. Fish-odour syndrome and impaired n-oxidation. Biochem Soc Trans 1996; 24(1): 965
Al-Waiz M, Ayesh R, Mitchell SC, et al. Disclosure of the metabolic retroversion of trimethylamine N-oxide in humans: a pharmacogenetic approach. Clin Pharmacol Ther 1987; 42(6): 608–12
Zeisel S, Da Costa K, Ox J. Endogenous formation of dimethylamine. Biochem J 1975; 232(2): 403–8
Mitchell SC, Zhang A, Smith RL, et al. Trimethylamine noxide as a probable source of urinary dimethylamine in man; a statistical approach. Biochem Soc Trans 1998; 26(2): S182
Zhang AQ, Mitchell SC, Ayesh R, et al. Dimethylamine formation in man. Biochem Pharmacol 1993; 45(11): 2185–8
Simenhoff ML. Metabolism and toxicity of aliphatic amines. Kidney Int Suppl 1975; 7: S314–7
Simenhoff ML, Burke JF, Saukkonen JJ, et al. Biochemical profile of uremic breath. N Engl J Med 1977; 297(3): 132–5
Simenhoff ML, Ginn HE, Teschan PE. Toxicity of aliphatic amines in uremia. Trans Am Soc Artif Intern Organs 1977; 23: 560–5
McConnell HW, Mitchell SC, Smith RL, et al. Trimethylaminuria associated with seizures and behavioural disturbance: a case report. Seizure 1997; 6(4): 317–22
Hokland BM, Bremer J. Metabolism and excretion of carnitine and acylcarnitines in the perfused rat kidney. Biochim Biophys Acta 1986; 886(2): 223–30
Rebouche CJ, Engel AG. Carnitine metabolism and deficiency syndrome. Mayo Clin Proc 1983 Aug; 58: 533–40
Pons R, Carrozzo R, Tein I, et al. Deficient muscle carnitine transport in primary carnitine deficiency. Pediatr Res 1997; 42: 583–7
Rebouche CJ, Paulson DJ. Carnitine metabolism and function in humans. Annu Rev Nutr 1986; 6: 41–66
Rose SJ, Stokes TC, Patel S, et al. Carnitine deficiency associated with long-term pivampicillin treatment: the effect of a replacement therapy regime. Postgrad Med J 1992; 68: 932–4
Holme E, Jodal U, Linstedt S, et al. Effects of pivalic acidcontaining prodrugs on carnitine homeostasis and on response to fasting in children. Scand J Clin Lab Invest 1992; 52(5): 361–72
Dalakas MC, Leon-Monzon ME, Bernardini I, et al. Zidovudine-induced mitochondrial myopathy is associated with muscle carnitine deficiency and lipid storage. Ann Neurol 1994; 35(4): 482–7
Ross NS, Hoppel CL. Partial muscle carnitine palmitoyltransferase-A deficiency. JAMA 1987; 257(1): 62–5
Ganapathy V, Huang W, Rajan DP, et al. Beta-lactam antibiotics as substrates for OCTN2, an organic cation/carnitine transporter. J Biol Chem 2000; 275(3): 1699–707
Ohtani Y, Nishiyama S, Matsuda I. Renal handling of free and acylcarnitine in secondary carnitine deficiency. Neurology 1984; 34(7): 977–9
Krahenbuhl S, Reichen J. Carnitine metabolism in patients with chronic liver disease. Hepatology 1996; 25: 148–53
Bartel LL, Hussey JL, Shrago E. Perturbation of serum carnitine levels in human adults by chronic renal disease and dialysis therapy. Am J Clin Nutr 1981 Jul; 34: 1314–20
Rodríguez-Segade S, de la Peña CA, Paz M, et al. Carnitine concentrations in dialysed and undialysed patients with chronic renal insufficiency. Ann Clin Biochem 1986; 23: 671–5
Ahmad S. Carnitine, kidney and renal dialysis: carnitine and the kidney. In: Ferrari R, Dimauro S, Sherwood G, editors. L-Carnitine and its role in medicine: from function to therapy. New York: Academic Press, 1992: 381–400
Bohmer T, Bergrem H, Eiklid K. Carnitine deficiency induced during intermittent haemodialysis for renal failure. Lancet 1978; I(8056): 126–8
Penn D, Schmidt-Sommerfeld E. Carnitine and carnitine esters in plasma and adipose tissue of chronic uremic patients undergoing hemodialysis. Metabolism 1983; 32(8): 806–9
Vacha GM, Corsi M, Giorcelli G, et al. Serum and muscle Incarnitine levels in hemodialyzed patients during and after longterm L-carnitine treatment. CurrTher Res 1985; 37(3): 505–16
Jackson JM, Lee HA. L-Carnitine and acetyl-L-carnitine status during hemodialysis with acetate in humans: a kinetic analysis. Am J Clin Nutr 1996; 64: 922–7
Guarnieri G, Toigo G, Crapesi L, et al. Carnitine metabolism in chronic renal failure. Kidney Int Suppl 1987; 32 Suppl. 22: S 116–27
Sakurauchi Y, Matsumoto Y, Shinzato T, et al. Effects of Incarnitine supplementation on muscular symptoms in hemodialyzed patients. Am J Kidney Dis 1998; 32(2): 258–64
Golper TA, Wolfson M, Ahmad S, et al. Multicenter trial of Incarnitine in maintenance hemodialysis patients: I. carnitine concentrations and lipid effects. Kidney Int 1990; 38: 904–11
Siami G, Clinton ME, Mrak R, et al. Evaluation of the effect of intravenous L-carnitine therapy on function structure and fatty acid metabolism of skeletal muscle in patients receiving chronic hemodialysis. Nephron 1991; 57: 306–13
Kurtin PS. Carnitine metabolism and usage in chronic dialysis patients. Semin Dial 1990; 3(3): 166–70
Leschke M, Rumpf KW, Eisenhauer T, et al. Quantitative assessment of carnitine loss during hemodialysis and hemofiltration. Kidney Int Suppl 1983; 24 Suppl. 16: S143–6
Ricanti ES, Hoppel CL. Hemodialysis (HD) in chronic renal failure (CRF) does not produce carnitine (c) depletion [abstract]. Kidney Int 1985, 170
Ahmad S, Robertson HT, Golper TA, et al. Multicenter trial of L-carnitine in maintenance hemodialysis patients: II. clinical and biochemical effects. Kidney Int 1990; 38: 912–8
Acknowledgements
The authors would like to thank Ms Wave Sewlall, Ms Lauren Graham and Ms Judy Inge for their expert assistance in the preparation of this manuscript. There were no sources of funding or conflicts of interest directly relevant to the content of this review.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Evans, A.M., Fornasini, G. Pharmacokinetics of L-Carnitine. Clin Pharmacokinet 42, 941–967 (2003). https://doi.org/10.2165/00003088-200342110-00002
Published:
Issue Date:
DOI: https://doi.org/10.2165/00003088-200342110-00002