Screening of cellulolytic activity of some Rhizospheric soil fungi Isolated from different sources

Document Type : Research and Reference

Authors

1 Botany Department, Faculty of Science, Tanta University

2 botany and microbiology department, faculty of science , Tanta university

Abstract

Cellulases are enzymes that catalyze the bioconversion of cellulose to reduce sugars and glucose. Most fungal cellulase enzymes degrade the cellulose molecule and several other polysaccharides into monosaccharides. In this study, twenty fungal species were isolated from several locations in Al-Gharbia governorate, Egypt. The soil samples are from various plant rhizospheres (rice, wheat, bean, sugar beet, and pea).These fungi were identified from seven different genera "Trichoderma, Aspergillus, Rhizopus, Cephalosporium, Fusarium, Penicillium, Mucor". All identified fungal species were tested for cellulolytic activity in the solid medium. Out of twenty fungal species, seventeen species showed cellulolytic activity and the highest activity was recorded in Trichoderma viride (67.2 U/ml) and Aspergillus sydawi (52.1 U/ml). The cellulolytic fungi were selected to estimate the carboxymethyl cellulase (CMCase), β-glucosidase (βGase) and filter paper cellulase (FPase) in the liquid medium. All tested fungi showed significant activity for the three enzymes. Trichoderma viride had the highest total cellulolytic activity of the three cellulolytic enzymes.

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